iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Sly-111660

Ensembl Protein ID

Solyc12g005870.1.1

UniProt Accession

K4DBC5; K4DBC5_SOLLC

Protein Name

NEDD8-activating enzyme E1 regulatory subunit

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
Solyc12g005870.1	Solyc12g005870.1.1	Solyc12g005870.1.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E1	1.50e-112	377.6	8	497

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

   S: 1    YDRQirlwGeeaqeklksakvllvGagglGcEllKnLvlaGvgsitvvDmdtvevsnlnrqFLfraedvgksKAevaaealkelnpdvnve 91
           YDRQ+r+wGe++q++l++a+++l+++g++G+E+lKnLvl+Gvgsit+vD+++vev +l+++F++++++vg+sKAe+++++l+eln+ v+++
   Q: 8    YDRQLRIWGEKGQAALEKASICLLNCGPTGSETLKNLVLGGVGSITIVDGSKVEVGDLGNNFMVDESSVGQSKAECVCAFLQELNDAVKAK 98
           ******************************************************************************************* PP
   S: 92   aheesiteleeeiv.FfkkfdvVvlaldnrearrkvnrlclnarvpliesgtlGllGqvrviikgltecyscsd...dppqktiPfctlke 178
           + ee ++el e+++ Ff++f++V++++  ++++ k++r+c++a++ li ++++Gl+G vr+++k++t+++s++d   d+++++ P+++l+ 
   Q: 99   FIEEHPEELIETNPsFFSQFTLVIATQLVEDSMVKLDRICREANIILIFARSYGLMGLVRISVKEHTVIESKPDhflDDLRLNDPWPELRR 189
           ******************************************************************************************* PP
   S: 179  tpn.........aaehtiewavlfnklleeeageeevlekldseekeegkdkvkselksedeenfeeaieiavkafakttins.ikqllks 259
           + +         +++++++++ ++ k++ee++  + ++ ++++eek+++kd++ks++ + deen++ea+e+++k f++  i+   ++++++
   Q: 190  FAEtidlnttdaVVHKHTPYIIILVKMAEEWTNMHGGKFPSTREEKKQFKDLIKSKMSTVDEENYKEAMEASFKVFSPVGIGPnLQKIIND 280
           *87788888888**********************************************************************999999999 PP
   S: 260  fecdivtkskspfwv...........................................................................s 275
            +c++v +++s+fwv                                                                           s
   Q: 281  -SCAEVDSNSSDFWVmvaamkefiasegggetplegsipdmtsstelyvnlqktyqakaeadflameqrvknllkkfsrdpasiskanikS 370
           .****************************************************************************************** PP
   S: 276  fcsnaealq...........vpekvekdeevvkasap.....lylllralerfekkkgrkpgelsfekdddsavdlvtaaanlraeslgie 350
           fc na++l              +   + +++++++++     ly+llra++rf++++++ pg+++ e+d+d + +l+t+a  l   +lg++
   Q: 371  FCRNARKLAvcryrlvedefNSPAQPELQKYLTDEDYgtaagLYILLRAADRFAANYNKFPGQFDGEMDED-ISRLKTTAVGLL-NDLGCN 459
           *********665555544442255555556666666688899*****************************.******999998.****** PP
   S: 351  p.kldddlikeiagniipaiattnavvgGlaaqEviKv 387
           +  +++dli e+++++++++++++a+vgG+ +qEviKv
   Q: 460  GsSVSEDLINEMCRYGASELHVVAAFVGGVTSQEVIKV 497
           *************************************8 PP

Organism

Solanum lycopersicum

Functional Description
(View)

Regulatory subunit of the dimeric E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1.

Protein Sequence
(Fasta)

MAEPKVKYDR QLRIWGEKGQ AALEKASICL LNCGPTGSET LKNLVLGGVG SITIVDGSKV 60
EVGDLGNNFM VDESSVGQSK AECVCAFLQE LNDAVKAKFI EEHPEELIET NPSFFSQFTL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGGCGGAAC CTAAAGTCAA ATATGATCGT CAGCTCAGGT GAATTTTTTA GTGTATTCTA 60
TGTTTTCTGC TTTCAGTTCT GTTATGAACG CCATCACTGA ATGTGTAGTT CTTATATAGT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-1185--Reference proteome
KW-0833--Ubl conjugation pathway

Interpro

IPR030667--APP-BP1
IPR016040--NAD(P)-bd_dom
IPR000594--ThiF_NAD_FAD-bd

Pfam

PF00899--ThiF

Gene Ontology

GO:0019781--F:NEDD8 activating enzyme activity
GO:0045116--P:protein neddylation

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ago-007900	Ashbya gossypii	25.19	4.00e-31	128.00
IUUC-Cpo-022248	Cavia porcellus	35.85	2.00e-67	248.00
IUUC-Dno-030033	Dasypus novemcinctus	40.87	2.00e-107	381.00
IUUC-Dor-030513	Dipodomys ordii	51.82	2.00e-27	116.00
IUUC-Ete-032420	Echinops telfairi	37.68	2.00e-104	372.00
IUUC-Ggr-040005	Gaeumannomyces graminis	34.10	5.00e-57	214.00
IUUC-Lpe-051535	Leersia perrieri	66.33	0.00e+00	666.00
IUUC-Mpo-057514	Magnaporthe poae	34.44	8.00e-57	214.00
IUUC-Mpu-066716	Mustela putorius furo	40.40	8.00e-116	410.00
IUUC-Opr-070552	Ochotona princeps	37.30	1.00e-95	342.00
IUUC-Ogu-078844	Oryza glumaepatula	67.81	0.00e+00	664.00
IUUC-Olo-080943	Oryza longistaminata	65.79	0.00e+00	644.00
IUUC-Oru-084960	Oryza rufipogon	65.73	0.00e+00	646.00
IUUC-Pab-098255	Pongo abelii	40.94	5.00e-103	367.00
IUUC-Pca-100807	Procavia capensis	49.73	7.00e-52	197.00
IUUC-Sce-106397	Saccharomyces cerevisiae	28.36	5.00e-30	124.00
IUUC-Sha-106770	Sarcophilus harrisii	46.69	1.00e-70	258.00
IUUC-Sar-113083	Sorex araneus	31.57	7.00e-78	283.00
IUUC-Sre-115058	Sporisorium reilianum	33.11	2.00e-66	246.00
IUUC-Tsy-119111	Tarsius syrichta	42.49	1.00e-60	226.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence