iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
Protein-protein Interaction
iRefIndex	PINA	HINT
Post-translational Modifications (PTMs)
dbPPT
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
DUB/USP	2.30e-71	241.6	168	473

Active Site

Position(s)	Description	Evidence
177	Nucleophile	ECO:0000255\|PROSITE-ProRule:PRU10092}
433	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092}

Domain Profile

DUB/USP

   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrellle.kkkeklneenpkepkgellkslqklfeelwkskkkavsprellkalkkeaeefsgyeqqDaqEfla 97
           +Gl N gN+C+ N +LQcLs +++l  +lle  +k+++ ++     + ++l+++q+ +e   ++++  +sp ++++ l ++   +   +q+Da+Ef++
   Q: 168  CGLMNCGNSCFANVILQCLSWTRPLVAYLLEkGHKRECMRN-----DWCFLCEFQTHVERA-SQSRFPFSPMNIISRLTNIGGTLGYGRQEDAHEFMR 259
           7******************************6666777665.....57**********999.88889******************************* PP
   S: 98   flldqleeelkkeknkpkeeeeeedkselsdeaeekkkkknkslisdlFqgqlksqikclecdnvssreepfldlslpvpgkeetkkeslqdcLesft 195
           + +d +++ +         +e++ +       +   + +++++li+ +F+g l+sq++c  c++vs ++e+++dl ++ +g+      sl++cL++ft
   Q: 260  YAIDMMQSVC--------LDEFGGE-------KIVPPRSQETTLIQYIFGGLLQSQVQCTVCNHVSDQYENMMDLIVEMHGDAG----SLEECLDQFT 338
           **********........4444444.......56789999*******************************************5....8********* PP
   S: 196  keeelegenkyycekckkkqeaekklklkklPevLilhLkRfsfseeqksrekinkkvefpleLdlspfctspeekqkekkkYeLiavvvHsGslss. 292
           +ee l+g+n+y+c++c ++++a k+l++++ P++L++ LkR+    ++++  k+nk+++fp++Ldl+p++++  ++   ++ Y+L+av+vH   l+  
   Q: 339  AEEWLHGDNMYKCDRCSDYVKACKRLTIRRAPNILTIALKRY----QGGRYGKLNKRISFPETLDLNPYMSEGGDG---SDVYKLYAVIVHLDMLNAs 429
           ******************************************....678999********************6655...79**********9955544 PP
   S: 293  ..GHYvayvknekgkWllfdDskVtevkeeevleetgstaYvlfYkr 337
             GHY++y+k+  g+W+++dDs++++v+ e+vl+   + aY+l+Y r
   Q: 430  ffGHYICYIKDFCGNWYRIDDSEIESVELEDVLS---QRAYMLLYSR 473
           55********************************...9*******87 PP

Organism

Arabidopsis thaliana

Functional Description
(View)

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).

Protein Sequence
(Fasta)

MHEVGFPLDL SVFTRLIATL FFLAVGVFYF LKNTAAKYFD IGAAAAGGFD RDFMAVDAED 60
CSVCGNFSTK KCSRCKSVRY CSAECQRSDW SSGHQRNCRD YGITTLTPSA KNGLRFRASP 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATTCTTGAAC AGACAAAGAA AAAAAAAAGG TGAATACTTG AAATATATAA AAGATACAGA 60
AGACACGAGA AGGGAGAAGA GATCGATGAT TTTGAGAAGC GAATTAGATG CATGAGGTTG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0472--Membrane
KW-0479--Metal-binding
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0812--Transmembrane
KW-1133--Transmembrane helix
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR001394--Peptidase_C19_UCH
IPR018200--USP_CS
IPR028889--USP_dom
IPR002893--Znf_MYND

PROSITE

PS00972--USP_1
PS50235--USP_3
PS01360--ZF_MYND_1
PS50865--ZF_MYND_2

Pfam

PF00443--UCH
PF01753--zf-MYND

Gene Ontology

GO:0016021--C:integral component of membrane
GO:0046872--F:metal ion binding
GO:0036459--F:thiol-dependent ubiquitinyl hydrolase activity
GO:0016579--P:protein deubiquitination
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

ath:AT4G31670

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aly-005863	Arabidopsis lyrata	93.34	0.00e+00	1163.00
IUUC-Bol-016178	Brassica oleracea	77.48	0.00e+00	942.00
IUUC-Bra-018083	Brassica rapa	75.94	0.00e+00	924.00
IUUC-Cne-027049	Cryptococcus neoformans	31.72	4.00e-38	152.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence