iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Hsa-045853

UUCD1 version

UUC-HoS-00243

Ensembl Protein ID

ENSP00000356641.3

UniProt Accession

Q8NHY2; E9PKI0; Q504W6; Q6H103; Q9H6L7; X5D9B4; RFWD2_HUMAN

Genbank Protein ID

AAM34692.1; DAA01050.1; AAQ08989.1; AAS82851.1; AHW56568.1; AHW56715.1; BAB15239.1; BAG37289.1; CAH71140.1; CAH71140.1; CAH71140.1; CAH71140.1; CAH72425.1; CAH72425.1; CAH72425.1; CAH72425.1; CAH73625.1; CAH73625.1; CAH73625.1; CAH73625.1; CAI14643.1; CAI14643.1; CAI14643.1; CAI14643.1; EAW91000.1; AAH94728.1

Protein Name

E3 ubiquitin-protein ligase RFWD2; Constitutive photomorphogenesis protein 1 homolog; RING finger and WD repeat domain protein 2; RING finger protein 200; RING-type E3 ubiquitin transferase RFWD2

Genbank Nucleotide ID

AF508940; BK000438; AF527539; AY509921; KJ534928; KJ535076; AK025789; AK314750; AL359265; AL162736; AL513329; AL590723; AL513329; AL162736; AL359265; AL590723; AL162736; AL359265; AL513329; AL590723; AL590723; AL162736; AL359265; AL513329; CH471067; BC094728

Gene Name

RFWD2; COP1; RNF200

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000143207.19	ENST00000367667.5	ENSP00000356639.1
ENSG00000143207.19	ENST00000367669.7	ENSP00000356641.3
ENSG00000143207.19	ENST00000367666.5	ENSP00000356638.1
ENSG00000143207.19	ENST00000308769.12	ENSP00000310943.8
ENSG00000143207.19	ENST00000459744.5	ENSP00000437212.1
ENSG00000143207.19	ENST00000498306.5	ENSP00000433810.1
ENSG00000143207.19	ENST00000491600.5	ENSP00000437275.1
ENSG00000143207.19	ENST00000474194.1	ENSP00000433517.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	HPM
DNA & RNA Element
UTRdb	circBase	circRNADb	CircNet
miRTarBase	microRNA	TRANSFAC	miRWalk
TargetScan	RepTar	miRNAMap	SomamiR
miRcode	RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha	InWeb_IM
Protein 3D Structure
PDB
Disease-associated Information
GWASdb	GWAS Central	OMIM
Drug and target
TTD
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
BioGRID	mUbiSiDa
DNA Methylation
TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/RING/RING	RING	19643728; 18815134; 17588513; 19176998; 19007436; 14739464; 19741714; 21403399
E3 adaptor/Cullin RING/DCX/DWD	WD_repeat	19643728; 18815134; 17588513; 14739464; 19741714; 21403399

Classification

Family	E-value	Score	Start	End
E3 activity/RING/RING	6.60e-06	28.2	136	174
E3 adaptor/Cullin RING/DCX/DWD	1.20e-23	83.8	470	499

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 1    CaiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           C+iC++++++  + + CgH f+ +C+++          + rCP C+
   Q: 136  CPICFDMIEE-AYMTKCGHSFCYKCIHQS------LEDNNRCPKCN 174
           ********99.888***********9555......999999****6 PP

E3 adaptor/Cullin RING/DCX/DWD

   S: 3    tdsvtclaf..sd.tllvsGsdDgtikvWD 29
           +++++c+++     +ll+s+ + gt+ +WD
   Q: 470  NSKISCISWssYHkNLLASSDYEGTVILWD 499
           689******9622599************** PP

Organism

Homo sapiens

Functional Description
(View)

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).

Protein Sequence
(Fasta)

MSGSRQAGSG SAGTSPGSSA ASSVTSASSS LSSSPSPPSV AVSAAALVSG GVAQAAGSGG 60
LGGPVRPVLV APAVSGSGGG AVSTGLSRHS CAARPSAGVG GSSSSLGSGS RKRPLLAPLC 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

CACCTATACC ATTCATTCCA GTCTCCTCAA TTCTTTCACT GTATTAAGAC GGCCTGCCCT 60
CTGGCACGTT TTCTGGAACG AAAGCGGCTT GGGACAGACA GACTCCCTTC GCCAGGGAGC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0025--Alternative splicing
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0677--Repeat
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0853--WD repeat
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR015943--WD40/YVTN_repeat-like_dom
IPR001680--WD40_repeat
IPR019775--WD40_repeat_CS
IPR017986--WD40_repeat_dom
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS

PROSITE

PS00678--WD_REPEATS_1
PS50082--WD_REPEATS_2
PS50294--WD_REPEATS_REGION
PS00518--ZF_RING_1
PS50089--ZF_RING_2

Pfam

PF00400--WD40

SMART

SM00184--RING
SM00320--WD40

Gene Ontology

GO:0005829--C:cytosol
GO:0000139--C:Golgi membrane
GO:0016607--C:nuclear speck
GO:0005654--C:nucleoplasm
GO:0061630--F:ubiquitin protein ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0008270--F:zinc ion binding
GO:0032436--P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0010212--P:response to ionizing radiation

KEGG

hsa:64326

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000885	Aegilops tauschii	40.09	2.00e-126	446.00
IUUC-Aml-001265	Ailuropoda melanoleuca	99.68	0.00e+00	1248.00
IUUC-Atr-002460	Amborella trichopoda	38.83	7.00e-125	441.00
IUUC-Apl-003796	Anas platyrhynchos	97.93	0.00e+00	1154.00
IUUC-Aca-005078	Anolis carolinensis	97.42	0.00e+00	1211.00
IUUC-Aly-006014	Arabidopsis lyrata	40.09	2.00e-130	460.00
IUUC-Ath-007636	Arabidopsis thaliana	39.85	3.00e-130	459.00
IUUC-Ame-011176	Astyanax mexicanus	75.27	8.00e-70	258.00
IUUC-Bta-012595	Bos taurus	97.21	0.00e+00	1271.00
IUUC-Bdi-014152	Brachypodium distachyon	39.14	2.00e-129	456.00
IUUC-Bol-016380	Brassica oleracea	40.43	2.00e-131	462.00
IUUC-Bra-017690	Brassica rapa	39.30	5.00e-126	445.00
IUUC-Cja-018926	Callithrix jacchus	99.38	0.00e+00	1261.00
IUUC-Cfa-021088	Canis familiaris	98.91	0.00e+00	1259.00
IUUC-Cpo-022442	Cavia porcellus	98.11	0.00e+00	1224.00
IUUC-Cre-022579	Chlamydomonas reinhardtii	33.45	3.00e-33	135.00
IUUC-Csa-024119	Chlorocebus sabaeus	97.69	0.00e+00	1251.00
IUUC-Cho-024883	Choloepus hoffmanni	66.89	5.00e-107	382.00
IUUC-Cin-025375	Ciona intestinalis	55.40	0.00e+00	680.00
IUUC-Cme-027350	Cyanidioschyzon merolae	41.62	1.00e-79	291.00
IUUC-Dre-028453	Danio rerio	87.72	0.00e+00	1083.00
IUUC-Dno-029887	Dasypus novemcinctus	98.75	0.00e+00	1249.00
IUUC-Dor-031110	Dipodomys ordii	89.94	0.00e+00	875.00
IUUC-Ete-033016	Echinops telfairi	84.34	0.00e+00	1038.00
IUUC-Eca-034403	Equus caballus	98.96	0.00e+00	1156.00
IUUC-Eeu-034690	Erinaceus europaeus	82.02	0.00e+00	959.00
IUUC-Fca-036475	Felis catus	98.62	0.00e+00	1162.00
IUUC-Fal-037189	Ficedula albicollis	98.49	0.00e+00	1191.00
IUUC-Gmo-039245	Gadus morhua	86.79	0.00e+00	1056.00
IUUC-Gga-040806	Gallus gallus	98.23	0.00e+00	1238.00
IUUC-Gac-042177	Gasterosteus aculeatus	88.16	0.00e+00	1103.00
IUUC-Gma-044187	Glycine max	40.64	3.00e-127	449.00
IUUC-Ggo-044430	Gorilla gorilla	99.15	0.00e+00	942.00
IUUC-Hvu-047750	Hordeum vulgare	39.40	1.00e-95	345.00
IUUC-Itr-048238	Ictidomys tridecemlineatus	99.58	1.00e-143	501.00
IUUC-Lch-050420	Latimeria chalumnae	92.20	0.00e+00	1152.00
IUUC-Lpe-051219	Leersia perrieri	38.82	3.00e-124	439.00
IUUC-Loc-052728	Lepisosteus oculatus	91.45	0.00e+00	1106.00
IUUC-Laf-053775	Loxodonta africana	98.60	0.00e+00	1258.00
IUUC-Meu-056595	Macropus eugenii	77.46	4.00e-176	611.00
IUUC-Mtr-058555	Medicago truncatula	40.42	3.00e-134	472.00
IUUC-Mga-059646	Meleagris gallopavo	97.60	0.00e+00	1152.00
IUUC-Mmr-060990	Microcebus murinus	99.22	0.00e+00	1255.00
IUUC-Mdo-062287	Monodelphis domestica	98.87	0.00e+00	1247.00
IUUC-Mmu-064097	Mus musculus	98.76	0.00e+00	1258.00
IUUC-Mac-065240	Musa acuminata	41.37	5.00e-136	478.00
IUUC-Mpu-066612	Mustela putorius furo	98.63	0.00e+00	1164.00
IUUC-Mlu-067967	Myotis lucifugus	96.82	0.00e+00	1164.00
IUUC-Nle-069543	Nomascus leucogenys	99.53	0.00e+00	1265.00
IUUC-Opr-070366	Ochotona princeps	83.64	0.00e+00	714.00
IUUC-Ont-071522	Oreochromis niloticus	82.15	0.00e+00	1097.00
IUUC-Oan-073409	Ornithorhynchus anatinus	98.45	0.00e+00	1158.00
IUUC-Ocu-073756	Oryctolagus cuniculus	98.88	0.00e+00	1229.00
IUUC-Oba-075149	Oryza barthii	39.23	4.00e-127	449.00
IUUC-Obr-076563	Oryza brachyantha	39.48	2.00e-127	449.00
IUUC-Ogl-077701	Oryza glaberrima	39.26	1.00e-127	451.00
IUUC-Ogu-078383	Oryza glumaepatula	39.26	1.00e-127	451.00
IUUC-Oin-079965	Oryza indica	37.05	1.00e-113	404.00
IUUC-Olo-080639	Oryza longistaminata	38.80	4.00e-125	442.00
IUUC-Ome-081821	Oryza meridionalis	38.93	1.00e-115	410.00
IUUC-Oni-082622	Oryza nivara	39.26	1.00e-127	451.00
IUUC-Opu-083295	Oryza punctata	38.96	2.00e-127	450.00
IUUC-Oru-084661	Oryza rufipogon	39.26	1.00e-127	451.00
IUUC-Osa-085766	Oryza sativa	39.26	1.00e-127	451.00
IUUC-Ola-086727	Oryzias latipes	82.65	0.00e+00	1109.00
IUUC-Olu-087702	Ostreococcus lucimarinus	42.86	5.00e-69	254.00
IUUC-Oga-088215	Otolemur garnettii	97.20	0.00e+00	1232.00
IUUC-Oar-089880	Ovis aries	98.90	0.00e+00	1237.00
IUUC-Ptr-090597	Pan troglodytes	100.00	0.00e+00	1274.00
IUUC-Pan-091987	Papio anubis	99.53	0.00e+00	1264.00
IUUC-Psi-094033	Pelodiscus sinensis	97.97	0.00e+00	1167.00
IUUC-Pma-094587	Petromyzon marinus	70.20	0.00e+00	799.00
IUUC-Ppa-095256	Physcomitrella patens	39.79	2.00e-133	469.00
IUUC-Pfo-097388	Poecilia formosa	86.84	0.00e+00	1070.00
IUUC-Pab-098029	Pongo abelii	100.00	0.00e+00	1274.00
IUUC-Pop-099319	Populus trichocarpa	39.38	4.00e-131	462.00
IUUC-Pca-100846	Procavia capensis	90.95	0.00e+00	1145.00
IUUC-Ppe-101250	Prunus persica	38.10	8.00e-121	427.00
IUUC-Pva-102407	Pteropus vampyrus	98.91	0.00e+00	1249.00
IUUC-Rno-105312	Rattus norvegicus	98.76	0.00e+00	1256.00
IUUC-Sha-106514	Sarcophilus harrisii	98.66	0.00e+00	768.00
IUUC-Smo-109418	Selaginella moellendorffii	39.85	7.00e-130	457.00
IUUC-Sit-110248	Setaria italica	38.92	3.00e-128	452.00
IUUC-Sly-110964	Solanum lycopersicum	40.55	9.00e-132	464.00
IUUC-Stu-112882	Solanum tuberosum	40.80	9.00e-133	467.00
IUUC-Sar-113230	Sorex araneus	71.86	0.00e+00	798.00
IUUC-Sbi-113962	Sorghum bicolor	37.04	2.00e-123	436.00
IUUC-Ssc-115987	Sus scrofa	99.11	2.00e-180	625.00
IUUC-Tgu-116471	Taeniopygia guttata	98.22	0.00e+00	1232.00
IUUC-Tru-118231	Takifugu rubripes	87.52	0.00e+00	1054.00
IUUC-Tsy-119415	Tarsius syrichta	98.66	0.00e+00	1171.00
IUUC-Tni-120893	Tetraodon nigroviridis	84.09	0.00e+00	1065.00
IUUC-Tca-121703	Theobroma cacao	41.27	8.00e-130	457.00
IUUC-Tae-125317	Triticum aestivum	41.14	2.00e-134	473.00
IUUC-Tur-126557	Triticum urartu	40.78	1.00e-129	456.00
IUUC-Tbe-127253	Tupaia belangeri	71.16	0.00e+00	847.00
IUUC-Ttr-129100	Tursiops truncatus	97.31	0.00e+00	1140.00
IUUC-Vpa-130613	Vicugna pacos	92.94	0.00e+00	1102.00
IUUC-Vvi-130880	Vitis vinifera	41.13	1.00e-123	437.00
IUUC-Xtr-132033	Xenopus tropicalis	91.64	0.00e+00	1239.00
IUUC-Xma-133421	Xiphophorus maculatus	86.68	0.00e+00	1067.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence