iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000020020.8	ENSMUST00000095333.5	ENSMUSP00000092975.4
ENSMUSG00000020020.8	ENSMUST00000216224.1	ENSMUSP00000149020.1

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	microRNA	TRANSFAC	RepTar
RAID2
Protein-protein Interaction
IID	PINA	HINT	Mentha
Post-translational Modifications (PTMs)
dbPAF	PhosphositePlus
Protein Expression/Proteomics

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
DUB/USP	5.60e-96	322.8	272	676

Active Site

Position(s)	Description	Evidence
281	Nucleophile	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}
635	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrellle............kkkeklneenp................................... 42
           tGl+NlgNtCYmNSvLQ+Ls++  +r+++l+                      +                                   
   Q: 272  TGLRNLGNTCYMNSVLQVLSHLLIFRQCFLKldlnqwlavaasD---------Karsykhsavteaaaqqmnegqekekgfvcsrhsgl 351
           7******************************8776666665541.........134444455566677788888888888888888888 PP
   S: 43   ......................kepkgellkslqklfeelwkskkkavsprellkalkkeaeefsgyeqqDaqEflaflldqleeelkk 109
                                 ++p  +l+++l+ lf+ +w++++  vsp ++l++++++ + f+gy+qqDaqEfl+ lld++++el  
   Q: 352  ssglsggaskgrnmeliqprepSSPYSSLCHELHILFQVMWSGEWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQREL-- 438
           888888888888888888877777777************************************************************.. PP
   S: 110  eknkpkeeeeeedkselsdeaeekkkkknkslisdlFqgqlksqikclecdnvssreepfldlslpvpgkeetkke.......slqdcL 191
                  e+++++  +l   ++++  ++  ++++++F+gq+ sq++cl+cdn+s + e+f+dlsl++p++ +++ +        ++d+L
   Q: 439  -------ETTGTKLPALIPTSQRRLIEQVLNVVNNIFHGQFLSQVTCLACDNKSDTIESFWDLSLEFPERYQCSGKdaasqpcLVTDML 520
           .......7888999999999************************************************888877668888888****** PP
   S: 192  esftkeeelegenkyycekckkkqe...........aekklklkklPevLilhLkRfsfseeqksrekinkkvefpleLdlspfctspe 269
           ++ft++e leg+  y c++c++k++           a+k+l++ +lP+vL lhLkRf++s + ++reki  +v f ++L+++p+c++++
   Q: 521  DKFTETEALEGKI-YMCDHCNSKRRkfssksvvfteAQKQLMICHLPQVLRLHLKRFRWSGR-NNREKIGVHVVFEETLNMEPYCCRET 607
           **********965.*********999*********************************998.7778********************99 PP
   S: 270  ekqke..kkkYeLiavvvHsG.slssGHYvayvknekgk.WllfdDskVtevkeeevleetgstaYvlfYkr 337
            +  +  +  Y+L+avv+H+G +++sGHY+ay++n++g+ W++++Dsk + +++eev +   ++aY+lfY++
   Q: 608  LNALRpeCFLYNLSAVVIHHGkGFGSGHYTAYCYNSEGGfWVHCNDSKLSMCTMEEVRK---AQAYILFYTQ 676
           88766669***************************9999********************...********98 PP

Organism

Mus musculus

Functional Description
(View)

Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.

Protein Sequence
(Fasta)

MDRCKHVEQL QLAQGHSILD PQKWYCMVCN TTESIWACLS CSHVACGKYI QEHALKHFQE 60
SSHPVAFEVN DMYAFCYLCN DYVLNDNAAG DLKSLRSTLS TIKSKKYPCV VPSDSVLHPV 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

AGCAGACGGT ATCGTCCTAG ACGATAAAAG GACCGACCTC TTAGTCTAAA GGCCAATTCG 60
GCGACTGATT TTAAGGACAT AATAGGTAAG TATGGGAGAA TCCGCTGATG AGGGTGGGAA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0131--Cell cycle
KW-0132--Cell division
KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0498--Mitosis
KW-0539--Nucleus
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR001394--Peptidase_C19_UCH
IPR018200--USP_CS
IPR028889--USP_dom
IPR013083--Znf_RING/FYVE/PHD
IPR001607--Znf_UBP

PROSITE

PS00972--USP_1
PS00973--USP_2
PS50235--USP_3
PS50271--ZF_UBP

Pfam

PF00443--UCH
PF02148--zf-UBP

SMART

SM00290--ZnF_UBP

Gene Ontology

GO:0005634--C:nucleus
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0008270--F:zinc ion binding
GO:0051301--P:cell division
GO:0007059--P:chromosome segregation
GO:0007067--P:mitotic nuclear division
GO:1904667--P:negative regulation of ubiquitin protein ligase activity
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0016579--P:protein deubiquitination
GO:0090266--P:regulation of mitotic cell cycle spindle assembly checkpoint

KEGG

mmu:327799

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001220	Ailuropoda melanoleuca	79.07	0.00e+00	1095.00
IUUC-Apl-003673	Anas platyrhynchos	67.88	0.00e+00	933.00
IUUC-Aca-004434	Anolis carolinensis	66.33	0.00e+00	903.00
IUUC-Ame-010986	Astyanax mexicanus	59.04	0.00e+00	809.00
IUUC-Bta-012774	Bos taurus	77.53	0.00e+00	1085.00
IUUC-Cja-019619	Callithrix jacchus	79.61	0.00e+00	1105.00
IUUC-Cfa-020957	Canis familiaris	79.35	0.00e+00	1115.00
IUUC-Csa-023177	Chlorocebus sabaeus	78.65	0.00e+00	1098.00
IUUC-Cho-024877	Choloepus hoffmanni	74.11	0.00e+00	655.00
IUUC-Dre-027878	Danio rerio	58.77	0.00e+00	798.00
IUUC-Dno-029832	Dasypus novemcinctus	79.47	0.00e+00	1112.00
IUUC-Ete-032627	Echinops telfairi	75.84	0.00e+00	1066.00
IUUC-Eca-033863	Equus caballus	74.86	0.00e+00	1033.00
IUUC-Eeu-035225	Erinaceus europaeus	73.17	0.00e+00	1011.00
IUUC-Fca-036288	Felis catus	78.79	0.00e+00	1093.00
IUUC-Fal-037207	Ficedula albicollis	68.47	0.00e+00	929.00
IUUC-Gga-040457	Gallus gallus	66.99	0.00e+00	910.00
IUUC-Ggo-044926	Gorilla gorilla	79.18	0.00e+00	1097.00
IUUC-Hsa-046079	Homo sapiens	79.04	0.00e+00	1099.00
IUUC-Itr-048737	Ictidomys tridecemlineatus	80.17	0.00e+00	1103.00
IUUC-Lch-050260	Latimeria chalumnae	61.06	0.00e+00	818.00
IUUC-Loc-051981	Lepisosteus oculatus	71.86	4.00e-178	618.00
IUUC-Laf-054491	Loxodonta africana	77.63	0.00e+00	1031.00
IUUC-Mcc-055694	Macaca mulatta	78.93	0.00e+00	1099.00
IUUC-Mga-059523	Meleagris gallopavo	66.71	0.00e+00	920.00
IUUC-Mmr-060885	Microcebus murinus	80.65	0.00e+00	1111.00
IUUC-Mdo-063018	Monodelphis domestica	70.24	0.00e+00	962.00
IUUC-Mpu-066207	Mustela putorius furo	78.23	0.00e+00	1105.00
IUUC-Mlu-066929	Myotis lucifugus	75.79	0.00e+00	997.00
IUUC-Nle-069313	Nomascus leucogenys	69.03	0.00e+00	913.00
IUUC-Opr-070375	Ochotona princeps	70.08	0.00e+00	944.00
IUUC-Oan-073030	Ornithorhynchus anatinus	69.69	0.00e+00	736.00
IUUC-Ocu-074836	Oryctolagus cuniculus	77.11	0.00e+00	1068.00
IUUC-Oga-088367	Otolemur garnettii	80.79	0.00e+00	1087.00
IUUC-Oar-089953	Ovis aries	79.07	0.00e+00	1106.00
IUUC-Ptr-090980	Pan troglodytes	79.04	0.00e+00	1097.00
IUUC-Pan-092748	Papio anubis	79.07	0.00e+00	1099.00
IUUC-Pab-098514	Pongo abelii	78.65	0.00e+00	1093.00
IUUC-Pva-102984	Pteropus vampyrus	78.36	0.00e+00	1059.00
IUUC-Rno-106019	Rattus norvegicus	88.97	0.00e+00	1232.00
IUUC-Sha-106965	Sarcophilus harrisii	70.10	0.00e+00	957.00
IUUC-Ssc-115299	Sus scrofa	77.81	0.00e+00	1098.00
IUUC-Tgu-117160	Taeniopygia guttata	66.62	0.00e+00	905.00
IUUC-Tbe-128084	Tupaia belangeri	76.16	0.00e+00	1055.00
IUUC-Ttr-128928	Tursiops truncatus	80.06	0.00e+00	1115.00
IUUC-Vpa-130706	Vicugna pacos	78.65	0.00e+00	1099.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence