iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Mmu-063634

UUCD1 version

UUC-MuM-00060

Ensembl Protein ID

ENSMUSP00000082827.4

UniProt Accession

Q9CUN6; Q3U412; Q8K300; SMUF1_MOUSE

Genbank Protein ID

BAB29770.2; BAE32623.1; AAH29097.1

Protein Name

E3 ubiquitin-protein ligase SMURF1; HECT-type E3 ubiquitin transferase SMURF1; SMAD ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1

Genbank Nucleotide ID

AK015264; AK154491; BC029097

Gene Name

Smurf1

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000038780.14	ENSMUST00000110677.7	ENSMUSP00000106305.1
ENSMUSG00000038780.14	ENSMUST00000085684.10	ENSMUSP00000082827.4
ENSMUSG00000038780.14	ENSMUST00000100461.6	ENSMUSP00000098029.2
ENSMUSG00000038780.14	ENSMUST00000198621.1	ENSMUSP00000142841.1

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	microRNA	TRANSFAC	RepTar
RAID2
Protein-protein Interaction
IID	iRefIndex	PINA	HINT
Mentha
Post-translational Modifications (PTMs)
dbPAF	PhosphositePlus	dbPTM	BioGRID
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/HECT	HECT	20200942

Classification

Family	E-value	Score	Start	End
E3 activity/HECT	3.00e-127	425.8	395	730

Active Site

Position(s)	Description	Evidence
699	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00104}

Domain Profile

E3 activity/HECT

   S: 2    ksdlkkrlevefvgEegldlgGvtreflallskelqnpdlglflysendketlqpnpdsglknpehlklFeflGrllgkalqdgrlldl 90
           ++dlkkrl+v+f+gEegld+gGv+re+l+ll++e+ np++glf+ys ++ +tlq+npds + np+hl++F+f+Gr++g+a+++g++++ 
   Q: 395  PKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSI-NPDHLSYFHFVGRIMGLAVFHGHYING 482
           689*********************************************************9.89************************* PP
   S: 91   plsksfyklllgkklsledleevdpelykslkeikenaesdieelgldltfsvseeilgetkeeeLkpgGenipVtneNkeeYvklvve 179
            ++ +fyk+llgk+++l+dle+vdpel+ksl +i+en   di+   ld tf+v+++ +g + ++eLkp+G+n+pVt+eNk+eYv+l+v+
   Q: 483  GFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILEN---DITPV-LDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVN 567
           ***********************************99...9***8.******************************************* PP
   S: 180  yrlkegikeqveafreGfneviplellslFteeelellicGeeeidvedlkketkykggytedspaiqwFwevleefsseerkafLqFv 268
           +r+ +gi+ q+ a+++Gfne+ip++ll+ F+++elel+i G ++id++d+k++t+ k+ + +ds+ ++wFw+++e f++e r+++LqFv
   Q: 568  WRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKH-CVADSNIVRWFWQAVETFDEERRARLLQFV 655
           ********************************************************99.****************************** PP
   S: 269  TgssrlPlgGfaelk.......prlfvirk.dasddrlPssnTCfnyLkLPeYsskeilkekLlyAieetkgFel 335
           Tgs r+Pl+Gf++l+       prlf+i+  da++d+lP+++TCfn++++P+Y+s+e+l+ekLl+A+eet gF +
   Q: 656  TGSTRVPLQGFKALQgstgaagPRLFTIHLiDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAV 730
           ******************************99999**************************************76 PP

Organism

Mus musculus

Functional Description
(View)

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway (By similarity). Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Plays a role in dendrite formation by melanocytes (By similarity).

Protein Sequence
(Fasta)

MSNPGTRRNG SSIKIRLTVL CAKNLAKKDF FRLPDPFAKI VVDGSGQCHS TDTVKNTLDP 60
KWNQHYDLYV GKTDSITISV WNHKKIHKKQ GAGFLGCVRL LSNAISRLKD TGYQRLDLCK 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GCTCGGGAGG CGGCGGCGGA AGCAGCGAGG GCGGCGGGCG TCCGGCTGCG AGCGCGGCGG 60
CTCCGGGCTG GGCCAGCCAG GGGCGGCTCC TGGCGGCGGC GCGGCCGAGC GCTCGGTTCT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0221--Differentiation
KW-1017--Isopeptide bond
KW-0472--Membrane
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR000008--C2_dom
IPR000569--HECT_dom
IPR001202--WW_dom

PROSITE

PS50004--C2
PS50237--HECT
PS01159--WW_DOMAIN_1
PS50020--WW_DOMAIN_2

Pfam

PF00168--C2
PF00632--HECT
PF00397--WW

SMART

SM00239--C2
SM00119--HECTc
SM00456--WW

Gene Ontology

GO:0030424--C:axon
GO:0005737--C:cytoplasm
GO:0005829--C:cytosol
GO:0070062--C:extracellular exosome
GO:0005739--C:mitochondrion
GO:0043025--C:neuronal cell body
GO:0005886--C:plasma membrane
GO:0070411--F:I-SMAD binding
GO:0070412--F:R-SMAD binding
GO:0061630--F:ubiquitin protein ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0030509--P:BMP signaling pathway
GO:0030154--P:cell differentiation
GO:0061736--P:engulfment of target by autophagosome
GO:0030279--P:negative regulation of ossification
GO:0030512--P:negative regulation of transforming growth factor beta receptor signaling pathway
GO:0061734--P:parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization
GO:1903861--P:positive regulation of dendrite extension
GO:2000060--P:positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0006611--P:protein export from nucleus
GO:0034394--P:protein localization to cell surface
GO:0000209--P:protein polyubiquitination
GO:0071211--P:protein targeting to vacuole involved in autophagy
GO:0016567--P:protein ubiquitination
GO:0042787--P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0032801--P:receptor catabolic process
GO:0061753--P:substrate localization to autophagosome
GO:0030579--P:ubiquitin-dependent SMAD protein catabolic process

KEGG

mmu:75788

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001459	Ailuropoda melanoleuca	92.28	0.00e+00	1403.00
IUUC-Apl-004155	Anas platyrhynchos	92.68	0.00e+00	1412.00
IUUC-Aca-005238	Anolis carolinensis	94.82	0.00e+00	1409.00
IUUC-Ame-010901	Astyanax mexicanus	90.72	0.00e+00	1367.00
IUUC-Bta-013221	Bos taurus	90.54	0.00e+00	1329.00
IUUC-Cja-018985	Callithrix jacchus	95.38	0.00e+00	1469.00
IUUC-Cfa-020568	Canis familiaris	93.37	0.00e+00	1431.00
IUUC-Cpo-022531	Cavia porcellus	94.98	0.00e+00	1462.00
IUUC-Csa-024145	Chlorocebus sabaeus	98.08	0.00e+00	1470.00
IUUC-Cho-024963	Choloepus hoffmanni	83.65	0.00e+00	772.00
IUUC-Dre-028341	Danio rerio	91.13	0.00e+00	1377.00
IUUC-Dno-029705	Dasypus novemcinctus	93.48	0.00e+00	1419.00
IUUC-Dme-031740	Drosophila melanogaster	72.54	1.00e-175	610.00
IUUC-Eca-034125	Equus caballus	94.99	0.00e+00	1429.00
IUUC-Fca-035905	Felis catus	96.96	0.00e+00	1439.00
IUUC-Fal-037336	Ficedula albicollis	95.82	0.00e+00	1403.00
IUUC-Gmo-039486	Gadus morhua	80.92	0.00e+00	866.00
IUUC-Gga-040218	Gallus gallus	95.69	0.00e+00	1449.00
IUUC-Gac-041707	Gasterosteus aculeatus	84.71	0.00e+00	1267.00
IUUC-Ggo-045408	Gorilla gorilla	88.90	0.00e+00	1319.00
IUUC-Hsa-045878	Homo sapiens	95.24	0.00e+00	1469.00
IUUC-Itr-048317	Ictidomys tridecemlineatus	95.26	0.00e+00	1431.00
IUUC-Loc-052628	Lepisosteus oculatus	85.53	0.00e+00	1351.00
IUUC-Laf-053644	Loxodonta africana	92.73	0.00e+00	1388.00
IUUC-Mcc-055479	Macaca mulatta	90.29	0.00e+00	1321.00
IUUC-Mga-059723	Meleagris gallopavo	92.30	0.00e+00	1382.00
IUUC-Mdo-063005	Monodelphis domestica	97.40	0.00e+00	1468.00
IUUC-Mpu-065898	Mustela putorius furo	97.49	0.00e+00	1443.00
IUUC-Mlu-067584	Myotis lucifugus	93.97	0.00e+00	1425.00
IUUC-Nle-069033	Nomascus leucogenys	95.13	0.00e+00	1431.00
IUUC-Ont-072265	Oreochromis niloticus	83.05	0.00e+00	1280.00
IUUC-Oan-073621	Ornithorhynchus anatinus	90.81	0.00e+00	1407.00
IUUC-Ocu-073960	Oryctolagus cuniculus	93.79	0.00e+00	1416.00
IUUC-Oga-088579	Otolemur garnettii	95.24	0.00e+00	1466.00
IUUC-Oar-090396	Ovis aries	92.73	0.00e+00	1333.00
IUUC-Ptr-091624	Pan troglodytes	95.24	0.00e+00	1469.00
IUUC-Pan-092479	Papio anubis	94.99	0.00e+00	1432.00
IUUC-Pfo-097203	Poecilia formosa	84.59	0.00e+00	1276.00
IUUC-Pab-097540	Pongo abelii	95.03	0.00e+00	1399.00
IUUC-Pca-100379	Procavia capensis	73.16	0.00e+00	644.00
IUUC-Pva-102456	Pteropus vampyrus	85.36	0.00e+00	1232.00
IUUC-Rno-105396	Rattus norvegicus	99.86	0.00e+00	1496.00
IUUC-Sha-107216	Sarcophilus harrisii	93.35	0.00e+00	1432.00
IUUC-Ssc-116413	Sus scrofa	95.88	0.00e+00	1402.00
IUUC-Tgu-116723	Taeniopygia guttata	92.72	0.00e+00	1399.00
IUUC-Tru-117843	Takifugu rubripes	82.61	0.00e+00	1263.00
IUUC-Tsy-119455	Tarsius syrichta	94.90	0.00e+00	1356.00
IUUC-Tni-120037	Tetraodon nigroviridis	81.53	0.00e+00	1206.00
IUUC-Tbe-127879	Tupaia belangeri	81.45	0.00e+00	1172.00
IUUC-Ttr-128423	Tursiops truncatus	93.63	0.00e+00	1364.00
IUUC-Xtr-132237	Xenopus tropicalis	87.30	0.00e+00	1355.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence