iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

mRNA Expression
GEO	FFGED
DNA & RNA Element
TRANSFAC
Protein-protein Interaction
IID	iRefIndex	HINT	Mentha
Protein 3D Structure
PDB	MMDB
Post-translational Modifications (PTMs)
CPLM	dbPAF	PHOSIDA	BioGRID
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/HECT	HECT	12867034; 17719829; 20028738; 18094052; 19176477; 18367543; 18489705; 17475771; 17526739; 17429078; 19682071; 19433580; 18434603; 12654912; 15713680; 21242292; 15086787; 19759160; 14657247; 19168755; 17182850; 14657252; 15466864; 16338358; 17645432; 12872131; 25042851; 25620559

Classification

Family	E-value	Score	Start	End
E3 activity/HECT	5.60e-125	415.6	477	808

Active Site

Position(s)	Description	Evidence
777	Glycyl thioester intermediate	N/A

Domain Profile

E3 activity/HECT

   S: 2    ksdlkkrlevefvgEegldlgGvtreflallskelqnpdlglflysendketlqpnpdsglknpehlklFeflGrllgkalqdgrlldlplsksfyklllgk 103
           ++dlkkrl+++f gEegld+gGv+ref++lls+e++np ++lf+ys+ d++t+q+np+sg+ npehl++F+f+Gr++g+ ++++r+ld+++  ++yk++l+k
   Q: 477  PEDLKKRLMIKFDGEEGLDYGGVSREFFFLLSHEMFNPFYCLFEYSAYDNYTIQINPNSGI-NPEHLNYFKFIGRVVGLGVFHRRFLDAFFVGALYKMMLRK 577
           589*********************************************************9.89************************************** PP
   S: 104  klsledleevdpelykslkeikenaesdieelgldltfsvseeilgetkeeeLkpgGenipVtneNkeeYvklvveyrlkegikeqveafreGfneviplel 205
           k+ l+d+e vd+e+y+sl++++en   +i+   ldltfs+++e +ge+ +++Lkp+G+ni+Vt  Nk+eYv+l++++r+ ++++eq++af +Gfne+ip++l
   Q: 578  KVVLQDMEGVDAEVYNSLNWMLEN---SIDGV-LDLTFSADDERFGEVVTVDLKPDGRNIEVTDGNKKEYVELYTQWRIVDRVQEQFKAFMDGFNELIPEDL 675
           **********************99...99998.********************************************************************* PP
   S: 206  lslFteeelellicGeeeidvedlkketkykggytedspaiqwFwevleefsseerkafLqFvTgssrlPlgGfaelk....prlfvirkdasddrlPssnT 303
           +++F+e+elelli G +eid+ed+kk+t y+g y+e++++iqwFw++++e+++e+r+++LqF+Tg+sr+P++Gf++l+    pr+f+i+k+++ ++lP+s+T
   Q: 676  VTVFDERELELLIGGIAEIDIEDWKKHTDYRG-YQESDEVIQWFWKCVSEWDNEQRARLLQFTTGTSRIPVNGFKDLQgsdgPRRFTIEKAGEVQQLPKSHT 776
           ******************************76.********************************************************************* PP
   S: 304  CfnyLkLPeYsskeilkekLlyAieetkgFel 335
           Cfn+ +LP+Y  ++ +k+kL++A+eet gF++
   Q: 777  CFNRVDLPQYVDYDSMKQKLTLAVEETIGFGQ 808
           ******************************86 PP

Organism

Saccharomyces cerevisiae

Functional Description
(View)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other cell surface proteins like GAP1, FUR4, MAL61, PMA1 and STE2. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription, pressure-induced differential regulation of the two tryptophan permeases TAT1 and TAT2 and sorting efficiency into multivesicular bodies. Also acts on RBP1. Plays a role in tolerance to o-dinitrobenzene. Involved in actin cytoskeleton organization and dynamics. Ubiquitinates the LAS17-binding proteins LSB1 and PIN3/LSB2 without directing them for degradation and affects LAS17 levels in a SLA1-dependent and LSB1/2-independent manner.

Protein Sequence
(Fasta)

MPSSISVKLV AAESLYKRDV FRSPDPFAVL TIDGYQTKST SAAKKTLNPY WNETFKFDDI 60
NENSILTIQV FDQKKFKKKD QGFLGVVNVR VGDVLGHLDE DTATSSGRPR EETITRDLKK 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGCCTTCAT CCATATCCGT CAAGTTAGTG GCTGCAGAGT CATTATATAA GAGGGACGTA 60
TTCCGTTCCC CGGATCCGTT TGCTGTTCTG ACTATTGATG GTTACCAAAC CAAATCTACA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0002--3D-structure
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0206--Cytoskeleton
KW-1017--Isopeptide bond
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0677--Repeat
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR000008--C2_dom
IPR024928--E3_ub_ligase_SMURF1
IPR000569--HECT_dom
IPR001202--WW_dom

PROSITE

PS50004--C2
PS50237--HECT
PS01159--WW_DOMAIN_1
PS50020--WW_DOMAIN_2

Pfam

PF00168--C2
PF00632--HECT
PF00397--WW

SMART

SM00239--C2
SM00119--HECTc
SM00456--WW

Gene Ontology

GO:0030479--C:actin cortical patch
GO:0005934--C:cellular bud tip
GO:0005737--C:cytoplasm
GO:0010008--C:endosome membrane
GO:0031234--C:extrinsic component of cytoplasmic side of plasma membrane
GO:0005794--C:Golgi apparatus
GO:0005634--C:nucleus
GO:0000151--C:ubiquitin ligase complex
GO:0035091--F:phosphatidylinositol binding
GO:0043130--F:ubiquitin binding
GO:0061630--F:ubiquitin protein ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0034644--P:cellular response to UV
GO:0006333--P:chromatin assembly or disassembly
GO:0032511--P:late endosome to vacuole transport via multivesicular body sorting pathway
GO:0007005--P:mitochondrion organization
GO:0016973--P:poly(A)+ mRNA export from nucleus
GO:0045807--P:positive regulation of endocytosis
GO:0045723--P:positive regulation of fatty acid biosynthetic process
GO:0032436--P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0048260--P:positive regulation of receptor-mediated endocytosis
GO:0045944--P:positive regulation of transcription from RNA polymerase II promoter
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0051865--P:protein autoubiquitination
GO:0006513--P:protein monoubiquitination
GO:0000209--P:protein polyubiquitination
GO:0016567--P:protein ubiquitination
GO:0042787--P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0032956--P:regulation of actin cytoskeleton organization
GO:0010794--P:regulation of dolichol biosynthetic process
GO:0032443--P:regulation of ergosterol biosynthetic process
GO:0031384--P:regulation of initiation of mating projection growth
GO:0010793--P:regulation of mRNA export from nucleus
GO:0010796--P:regulation of multivesicular body size
GO:0006808--P:regulation of nitrogen utilization
GO:0019220--P:regulation of phosphate metabolic process
GO:0032880--P:regulation of protein localization
GO:2000203--P:regulation of ribosomal large subunit export from nucleus
GO:2000232--P:regulation of rRNA processing
GO:2000238--P:regulation of tRNA export from nucleus
GO:2000235--P:regulation of tRNA processing
GO:0010795--P:regulation of ubiquinone biosynthetic process
GO:0042493--P:response to drug
GO:0034517--P:ribophagy
GO:0070086--P:ubiquitin-dependent endocytosis
GO:0006511--P:ubiquitin-dependent protein catabolic process
GO:0043162--P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

KEGG

sce:YER125W

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001314	Ailuropoda melanoleuca	48.24	7.00e-159	552.00
IUUC-Apl-003893	Anas platyrhynchos	48.07	7.00e-157	545.00
IUUC-Aca-005338	Anolis carolinensis	48.63	1.00e-162	564.00
IUUC-Ago-007742	Ashbya gossypii	90.42	0.00e+00	1106.00
IUUC-Acl-008273	Aspergillus clavatus	65.22	0.00e+00	1015.00
IUUC-Afl-008713	Aspergillus flavus	65.74	0.00e+00	1015.00
IUUC-Afu-009103	Aspergillus fumigatus	63.85	0.00e+00	1003.00
IUUC-Ani-009259	Aspergillus nidulans	64.71	0.00e+00	1012.00
IUUC-Ang-009738	Aspergillus niger	65.43	0.00e+00	1030.00
IUUC-Aor-010053	Aspergillus oryzae	75.92	3.00e-141	492.00
IUUC-Ate-010529	Aspergillus terreus	66.46	0.00e+00	1030.00
IUUC-Ame-010810	Astyanax mexicanus	54.04	5.00e-147	513.00
IUUC-Bgr-012148	Blumeria graminis	65.66	0.00e+00	988.00
IUUC-Bta-012679	Bos taurus	48.24	5.00e-159	553.00
IUUC-Bci-013785	Botrytis cinerea	64.42	0.00e+00	1016.00
IUUC-Cel-018299	Caenorhabditis elegans	45.56	2.00e-148	517.00
IUUC-Cja-018732	Callithrix jacchus	48.24	2.00e-158	551.00
IUUC-Cfa-021226	Canis familiaris	48.24	2.00e-158	551.00
IUUC-Cpo-021671	Cavia porcellus	48.24	8.00e-159	552.00
IUUC-Csa-023252	Chlorocebus sabaeus	48.80	4.00e-163	566.00
IUUC-Cho-024695	Choloepus hoffmanni	49.48	1.00e-163	568.00
IUUC-Cin-025212	Ciona intestinalis	49.90	1.00e-141	495.00
IUUC-Csv-026256	Ciona savignyi	50.00	1.00e-142	497.00
IUUC-Cgl-026642	Colletotrichum gloeosporioides	64.00	0.00e+00	987.00
IUUC-Cne-026849	Cryptococcus neoformans	59.79	0.00e+00	959.00
IUUC-Dre-027770	Danio rerio	52.27	2.00e-147	514.00
IUUC-Dno-029466	Dasypus novemcinctus	48.24	7.00e-159	552.00
IUUC-Dor-030138	Dipodomys ordii	47.40	1.00e-153	534.00
IUUC-Dse-031241	Dothistroma septosporum	65.45	0.00e+00	1021.00
IUUC-Dme-032041	Drosophila melanogaster	45.62	3.00e-147	513.00
IUUC-Ete-033145	Echinops telfairi	45.56	1.00e-145	508.00
IUUC-Eca-033445	Equus caballus	48.24	8.00e-159	552.00
IUUC-Eeu-035247	Erinaceus europaeus	47.57	8.00e-157	545.00
IUUC-Fca-035605	Felis catus	48.97	6.00e-163	565.00
IUUC-Fal-037126	Ficedula albicollis	49.48	1.00e-163	568.00
IUUC-Fox-037774	Fusarium oxysporum	64.66	0.00e+00	1000.00
IUUC-Fso-038393	Fusarium solani	64.66	0.00e+00	998.00
IUUC-Gmo-039674	Gadus morhua	46.89	1.00e-151	528.00
IUUC-Ggr-040107	Gaeumannomyces graminis	63.79	0.00e+00	995.00
IUUC-Gga-040703	Gallus gallus	48.58	3.00e-158	550.00
IUUC-Gac-042020	Gasterosteus aculeatus	47.83	9.00e-155	538.00
IUUC-Ggo-045332	Gorilla gorilla	48.41	5.00e-159	553.00
IUUC-Hsa-045968	Homo sapiens	48.41	4.00e-159	553.00
IUUC-Itr-048918	Ictidomys tridecemlineatus	48.64	1.00e-162	564.00
IUUC-Kpa-049273	Komagataella pastoris	71.34	0.00e+00	1126.00
IUUC-Lch-050443	Latimeria chalumnae	48.07	3.00e-158	550.00
IUUC-Loc-052662	Lepisosteus oculatus	52.90	2.00e-143	501.00
IUUC-Lma-052980	Leptosphaeria maculans	64.13	2.00e-170	590.00
IUUC-Laf-053850	Loxodonta africana	48.24	2.00e-158	551.00
IUUC-Mcc-055821	Macaca mulatta	48.41	4.00e-159	553.00
IUUC-Meu-055953	Macropus eugenii	40.68	2.00e-121	428.00
IUUC-Mor-057097	Magnaporthe oryzae	65.19	0.00e+00	1018.00
IUUC-Mpo-057442	Magnaporthe poae	73.44	0.00e+00	837.00
IUUC-Mla-058996	Melampsora laricipopulina	60.21	0.00e+00	956.00
IUUC-Mga-059661	Meleagris gallopavo	49.48	1.00e-163	568.00
IUUC-Mvi-060395	Microbotryum violaceum	78.17	0.00e+00	826.00
IUUC-Mmr-061135	Microcebus murinus	48.81	4.00e-163	566.00
IUUC-Mdo-062505	Monodelphis domestica	48.81	9.00e-163	565.00
IUUC-Mmu-064136	Mus musculus	48.24	5.00e-159	553.00
IUUC-Mpu-066765	Mustela putorius furo	48.24	2.00e-158	551.00
IUUC-Mlu-067565	Myotis lucifugus	48.41	2.00e-158	551.00
IUUC-Nfi-068427	Neosartorya fischeri	66.46	0.00e+00	1025.00
IUUC-Ncr-068918	Neurospora crassa	64.21	0.00e+00	978.00
IUUC-Nle-069244	Nomascus leucogenys	48.97	6.00e-163	565.00
IUUC-Opr-071044	Ochotona princeps	47.57	1.00e-156	545.00
IUUC-Ont-072226	Oreochromis niloticus	47.08	7.00e-160	555.00
IUUC-Oan-073019	Ornithorhynchus anatinus	46.82	2.00e-152	530.00
IUUC-Ocu-074160	Oryctolagus cuniculus	47.66	1.00e-156	545.00
IUUC-Ola-087467	Oryzias latipes	47.39	2.00e-154	538.00
IUUC-Oga-088460	Otolemur garnettii	48.24	1.00e-158	551.00
IUUC-Oar-090198	Ovis aries	48.41	7.00e-158	549.00
IUUC-Ptr-090939	Pan troglodytes	48.41	4.00e-159	553.00
IUUC-Pan-092284	Papio anubis	48.41	3.00e-159	553.00
IUUC-Psi-093645	Pelodiscus sinensis	48.07	1.00e-158	551.00
IUUC-Pma-094364	Petromyzon marinus	52.46	1.00e-145	507.00
IUUC-Pno-095118	Phaeosphaeria nodorum	62.96	0.00e+00	1004.00
IUUC-Pfo-096937	Poecilia formosa	48.89	1.00e-159	554.00
IUUC-Pab-098088	Pongo abelii	48.41	4.00e-159	553.00
IUUC-Pca-100596	Procavia capensis	50.62	1.00e-141	494.00
IUUC-Pva-102354	Pteropus vampyrus	48.24	2.00e-158	551.00
IUUC-Pgr-103620	Puccinia graminis	75.31	0.00e+00	808.00
IUUC-Ptt-103806	Puccinia triticina	75.61	0.00e+00	689.00
IUUC-Pte-104016	Pyrenophora teres	75.00	0.00e+00	914.00
IUUC-Pyt-104550	Pyrenophora triticirepentis	75.00	0.00e+00	914.00
IUUC-Rno-105318	Rattus norvegicus	48.24	5.00e-159	553.00
IUUC-Sha-107482	Sarcophilus harrisii	47.33	6.00e-155	539.00
IUUC-Sja-107941	Schizosaccharomyces japonicus	63.78	0.00e+00	1003.00
IUUC-Spo-108171	Schizosaccharomyces pombe	63.54	0.00e+00	1001.00
IUUC-Ssl-108514	Sclerotinia sclerotiorum	64.80	0.00e+00	1006.00
IUUC-Stu-112582	Solanum tuberosum	35.47	1.00e-54	206.00
IUUC-Sar-113761	Sorex araneus	48.64	5.00e-162	563.00
IUUC-Sre-114929	Sporisorium reilianum	68.81	0.00e+00	853.00
IUUC-Ssc-115368	Sus scrofa	47.13	4.00e-153	533.00
IUUC-Tgu-117130	Taeniopygia guttata	47.91	3.00e-156	543.00
IUUC-Tru-118442	Takifugu rubripes	47.86	8.00e-161	558.00
IUUC-Tsy-119492	Tarsius syrichta	48.24	1.00e-157	548.00
IUUC-Tni-119993	Tetraodon nigroviridis	47.30	1.00e-150	525.00
IUUC-Tre-122203	Trichoderma reesei	64.25	0.00e+00	989.00
IUUC-Tvi-122595	Trichoderma virens	64.05	0.00e+00	997.00
IUUC-Tme-126897	Tuber melanosporum	63.83	0.00e+00	971.00
IUUC-Tbe-127793	Tupaia belangeri	49.38	1.00e-136	478.00
IUUC-Ttr-128275	Tursiops truncatus	48.03	1.00e-155	541.00
IUUC-Uma-129332	Ustilago maydis	68.64	0.00e+00	821.00
IUUC-Vda-129753	Verticillium dahliae	64.04	0.00e+00	966.00
IUUC-Vpa-130503	Vicugna pacos	50.73	2.00e-139	488.00
IUUC-Xtr-132859	Xenopus tropicalis	48.25	5.00e-159	553.00
IUUC-Xma-133797	Xiphophorus maculatus	48.66	3.00e-157	547.00
IUUC-Yli-134668	Yarrowia lipolytica	71.70	0.00e+00	926.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence