iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

UUCD2 ID

IUUC-Aml-001220

UUCD1 version

Ensembl Protein ID

UniProt Accession

Genbank Protein ID

Protein Name

Ubiquitin carboxyl-terminal hydrolase 44; Deubiquitinating enzyme 44; Ubiquitin thioesterase 44; Ubiquitin-specific-processing protease 44

Genbank Nucleotide ID

GL192660

Gene Name

USP44; PANDA_007904

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSAMEG00000002189.1	ENSAMET00000002387.1	ENSAMEP00000002292.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
DUB/USP	6.90e-98	328.0	272	676

Active Site

Position(s)	Description	Evidence
281	Nucleophile	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}
635	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrellle...........kkkeklneenp.................................... 42
           tGl+NlgNtCYmNSvLQ+Ls++  +r+++l+           ++        +                                    
   Q: 272  TGLRNLGNTCYMNSVLQVLSHLLIFRQCFLKldlnqwlavtaSD--------Ktrspykhpsitdtvyqmnecqetdtgsapsrhpsls 352
           7******************************8776655554422........1444555668888888899999888899999999999 PP
   S: 43   .....................kepkgellkslqklfeelwkskkkavsprellkalkkeaeefsgyeqqDaqEflaflldqleeelkke 110
                                +++  +l+++l++lf+ +w++k+  vsp ++l++++++ + f+gy+qqDaqEfl+ lld+++ el   
   Q: 353  lglsggasksrkmeliqprepSSQYISLCHELHTLFQVMWSGKWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQHEL--- 438
           99999888888888887777666677************************************************************... PP
   S: 111  knkpkeeeeeedkselsdeaeekkkkknkslisdlFqgqlksqikclecdnvssreepfldlslpvpgkeetkke.......slqdcLe 192
                 e+++++  +l   +++k  k+  ++++++F+gql sq++cl+cdn+s++ epf+dlsl++p++ +++ +        ++++L+
   Q: 439  ------ETTGTRLPALIPTSQRKLIKQVLNVVNNIFHGQLLSQVTCLACDNKSNTIEPFWDLSLEFPERYQCNGKdiasqpcRVTEMLA 521
           ......7788888999999************************************************999887778999999******* PP
   S: 193  sftkeeelegenkyycekckkkqe...........aekklklkklPevLilhLkRfsfseeqksrekinkkvefpleLdlspfctspee 270
           +ft++e leg+  y c++c++k++           a+k+l++ +lP+vL lhLkRf++s + ++reki  +v f + L+++p+c++++ 
   Q: 522  KFTETEALEGKI-YVCDHCNSKRRrfssksvvlteAQKQLMICHLPQVLRLHLKRFRWSGR-NNREKIGVHVGFEEILNMEPYCCRESL 608
           *********965.*******999999999999999***********************998.7778********************998 PP
   S: 271  kqke..kkkYeLiavvvHsG.slssGHYvayvknekgk.WllfdDskVtevkeeevleetgstaYvlfYkr 337
           k+ +  + +Y+L+avv+H+G +++sGHY+ay++n++g+ W++++Dsk + ++++ev++   ++aY+lfY++
   Q: 609  KSLRpeCFIYDLSAVVMHHGkGFGSGHYTAYCYNSEGGfWVHCNDSKLSMCTMDEVCK---AQAYILFYTQ 676
           8876779***************************9999********************...********98 PP

Organism

Ailuropoda melanoleuca

Functional Description
(View)

Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination (By similarity).

Protein Sequence
(Fasta)

MLTMDKCKHI GQLRLAQDHS ILNPQKWHCV DCNTTESIWA CLSCSHVACG RYIEEHALKH 60
FQESSHPVAL EVNEMYVFCY LCDDYVLNDN ATGDLKLLRS MLSAIKSQNY QCTTRSGRVL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGTTAACAA TGGATAAGTG CAAACACATT GGGCAGTTGC GGCTTGCTCA AGACCATTCC 60
ATCCTCAACC CTCAGAAGTG GCATTGTGTG GACTGCAATA CAACTGAGTC GATCTGGGCT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0131--Cell cycle
KW-0132--Cell division
KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0498--Mitosis
KW-0539--Nucleus
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR001394--Peptidase_C19_UCH
IPR018200--USP_CS
IPR028889--USP_dom
IPR013083--Znf_RING/FYVE/PHD
IPR001607--Znf_UBP

PROSITE

PS00972--USP_1
PS00973--USP_2
PS50235--USP_3
PS50271--ZF_UBP

Pfam

PF00443--UCH
PF02148--zf-UBP

SMART

SM00290--ZnF_UBP

Gene Ontology

GO:0005634--C:nucleus
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0008270--F:zinc ion binding
GO:0051301--P:cell division
GO:0007067--P:mitotic nuclear division
GO:1904667--P:negative regulation of ubiquitin protein ligase activity
GO:0043161--P:proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0016579--P:protein deubiquitination
GO:0090266--P:regulation of mitotic cell cycle spindle assembly checkpoint
GO:0090231--P:regulation of spindle checkpoint

KEGG

aml:100470180

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Apl-003673	Anas platyrhynchos	75.88	0.00e+00	1068.00
IUUC-Aca-004434	Anolis carolinensis	71.79	0.00e+00	1039.00
IUUC-Ame-010986	Astyanax mexicanus	73.36	0.00e+00	637.00
IUUC-Bta-012774	Bos taurus	90.72	0.00e+00	1304.00
IUUC-Cja-019619	Callithrix jacchus	90.32	0.00e+00	1290.00
IUUC-Cfa-020957	Canis familiaris	96.34	0.00e+00	1411.00
IUUC-Csa-023177	Chlorocebus sabaeus	90.18	0.00e+00	1291.00
IUUC-Cho-024877	Choloepus hoffmanni	90.13	0.00e+00	860.00
IUUC-Cin-025587	Ciona intestinalis	48.62	5.00e-108	385.00
IUUC-Csv-025814	Ciona savignyi	44.70	3.00e-154	538.00
IUUC-Dre-027878	Danio rerio	72.83	0.00e+00	627.00
IUUC-Dno-029832	Dasypus novemcinctus	92.41	0.00e+00	1347.00
IUUC-Ete-032627	Echinops telfairi	86.96	0.00e+00	1257.00
IUUC-Eca-033863	Equus caballus	86.04	0.00e+00	1234.00
IUUC-Eeu-035225	Erinaceus europaeus	86.10	0.00e+00	1222.00
IUUC-Fca-036288	Felis catus	96.20	0.00e+00	1400.00
IUUC-Fal-037207	Ficedula albicollis	75.32	0.00e+00	1027.00
IUUC-Gga-040457	Gallus gallus	75.35	0.00e+00	1029.00
IUUC-Gac-042595	Gasterosteus aculeatus	53.57	0.00e+00	703.00
IUUC-Ggo-044926	Gorilla gorilla	90.32	0.00e+00	1287.00
IUUC-Hsa-046079	Homo sapiens	90.04	0.00e+00	1285.00
IUUC-Itr-048737	Ictidomys tridecemlineatus	91.57	0.00e+00	1302.00
IUUC-Lch-050260	Latimeria chalumnae	68.26	0.00e+00	943.00
IUUC-Loc-051981	Lepisosteus oculatus	76.74	0.00e+00	675.00
IUUC-Laf-054491	Loxodonta africana	89.40	0.00e+00	1225.00
IUUC-Mcc-055694	Macaca mulatta	90.32	0.00e+00	1290.00
IUUC-Mga-059523	Meleagris gallopavo	75.46	0.00e+00	1053.00
IUUC-Mmr-060885	Microcebus murinus	93.13	0.00e+00	1325.00
IUUC-Mdo-063018	Monodelphis domestica	79.75	0.00e+00	1122.00
IUUC-Mmu-064012	Mus musculus	79.49	0.00e+00	1090.00
IUUC-Mpu-066207	Mustela putorius furo	95.78	0.00e+00	1413.00
IUUC-Mlu-066929	Myotis lucifugus	89.31	0.00e+00	1219.00
IUUC-Nle-069313	Nomascus leucogenys	80.17	0.00e+00	1109.00
IUUC-Opr-070375	Ochotona princeps	81.51	0.00e+00	1120.00
IUUC-Ont-071637	Oreochromis niloticus	68.50	3.00e-179	622.00
IUUC-Oan-073030	Ornithorhynchus anatinus	78.66	0.00e+00	852.00
IUUC-Ocu-074836	Oryctolagus cuniculus	87.89	0.00e+00	1244.00
IUUC-Oga-088367	Otolemur garnettii	93.13	0.00e+00	1308.00
IUUC-Oar-089953	Ovis aries	92.84	0.00e+00	1352.00
IUUC-Ptr-090980	Pan troglodytes	90.04	0.00e+00	1284.00
IUUC-Pan-092748	Papio anubis	91.01	0.00e+00	1304.00
IUUC-Psi-094078	Pelodiscus sinensis	76.78	0.00e+00	849.00
IUUC-Pfo-096413	Poecilia formosa	54.89	0.00e+00	732.00
IUUC-Pab-098514	Pongo abelii	90.32	0.00e+00	1291.00
IUUC-Pva-102984	Pteropus vampyrus	91.84	0.00e+00	1291.00
IUUC-Rno-106019	Rattus norvegicus	80.42	0.00e+00	1145.00
IUUC-Sha-106965	Sarcophilus harrisii	78.51	0.00e+00	1118.00
IUUC-Ssc-115299	Sus scrofa	93.39	0.00e+00	1365.00
IUUC-Tgu-117160	Taeniopygia guttata	75.58	0.00e+00	1030.00
IUUC-Tru-118156	Takifugu rubripes	61.43	0.00e+00	844.00
IUUC-Tni-119733	Tetraodon nigroviridis	62.16	0.00e+00	852.00
IUUC-Tbe-128084	Tupaia belangeri	85.96	0.00e+00	1240.00
IUUC-Ttr-128928	Tursiops truncatus	95.08	0.00e+00	1373.00
IUUC-Vpa-130706	Vicugna pacos	93.25	0.00e+00	1348.00
IUUC-Xtr-132061	Xenopus tropicalis	71.34	0.00e+00	942.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence