iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

iUUCD ID

IUUC-Pfo-097014

Ensembl Protein ID

ENSPFOP00000018977.2

UniProt Accession

A0A087YLS4; A0A087YLS4_POEFO

Genbank Protein ID

ENSPFOP00000018977

Protein Name

E3 ubiquitin-protein ligase RNF8; RING finger protein 8; RING-type E3 ubiquitin transferase RNF8

Genbank Nucleotide ID

AYCK01000253

Gene Name

rnf8; RNF8

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSPFOG00000018868.2	ENSPFOT00000018999.2	ENSPFOP00000018977.2

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E3 activity/RING/RING	8.40e-08	33.2	382	420

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 1    CaiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           C+iC e f +  ++l+C H f+  C+++w       +k++ CP+Cr
   Q: 382  CIICSELFIE-AVILNCAHSFCSYCIKQW------RNKKDECPICR 420
           ********99.888***************......**********8 PP

Organism

Poecilia formosa

Functional Description
(View)

E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains, leading to degradation of substrate proteins. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation.

Protein Sequence
(Fasta)

MDSVATDCCS AEEDDCSDSE VLCLMRVGKN SDWLRLFENA EITVGRGVEV THQLLSSTCP 60
LMISRLHCSF RQKEDGQWTV TDKKSLNGVW VNGERIPAEQ PHLLRLGDSI QLGVPLLGTK 120

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Nucleotide Sequence
(Fasta)

TAATAATAAT AATAATAATA ATAATAATAA TAGTTTATGT GTAGTGCTTT TATTTTGAAA 60
ATAAACTAAC TGTTTCCGGC TTCTCTGGTA GCGTTGCAGA GCTTCAACGC TAGCACAGTA 120

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Sequence Source

Ensembl

Keyword

KW-0156--Chromatin regulator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR000253--FHA_dom
IPR017335--RNF8
IPR008984--SMAD_FHA_domain
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS

PROSITE

PS50006--FHA_DOMAIN
PS00518--ZF_RING_1
PS50089--ZF_RING_2

Pfam

PF00498--FHA

SMART

SM00240--FHA
SM00184--RING

Gene Ontology

GO:0008270--F:zinc ion binding

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002124	Ailuropoda melanoleuca	38.91	1.00e-61	230.00
IUUC-Apl-003664	Anas platyrhynchos	69.32	1.00e-32	131.00
IUUC-Aca-005036	Anolis carolinensis	40.41	2.00e-69	256.00
IUUC-Ame-011537	Astyanax mexicanus	53.09	5.00e-114	404.00
IUUC-Bta-013297	Bos taurus	63.57	5.00e-38	152.00
IUUC-Cja-019201	Callithrix jacchus	39.41	3.00e-64	239.00
IUUC-Cfa-021041	Canis familiaris	39.03	4.00e-67	249.00
IUUC-Cre-022788	Chlamydomonas reinhardtii	28.72	9.00e-10	58.20
IUUC-Csa-024133	Chlorocebus sabaeus	42.62	2.00e-67	249.00
IUUC-Cho-024624	Choloepus hoffmanni	49.42	3.00e-22	99.40
IUUC-Dre-027918	Danio rerio	54.19	8.00e-119	420.00
IUUC-Dno-029774	Dasypus novemcinctus	40.54	1.00e-69	257.00
IUUC-Dor-030190	Dipodomys ordii	36.12	1.00e-48	187.00
IUUC-Ete-032301	Echinops telfairi	37.41	1.00e-44	174.00
IUUC-Eca-033607	Equus caballus	40.56	3.00e-58	219.00
IUUC-Eeu-034888	Erinaceus europaeus	48.59	1.00e-43	171.00
IUUC-Fca-036075	Felis catus	40.26	1.00e-68	254.00
IUUC-Fal-037569	Ficedula albicollis	40.37	4.00e-63	235.00
IUUC-Gmo-038588	Gadus morhua	57.54	3.00e-131	461.00
IUUC-Gga-040777	Gallus gallus	44.04	1.00e-70	260.00
IUUC-Gac-042463	Gasterosteus aculeatus	77.71	1.00e-162	566.00
IUUC-Ggo-045269	Gorilla gorilla	40.21	4.00e-67	249.00
IUUC-Hsa-046249	Homo sapiens	39.92	1.00e-63	237.00
IUUC-Itr-048314	Ictidomys tridecemlineatus	41.18	5.00e-71	261.00
IUUC-Lch-049805	Latimeria chalumnae	43.58	6.00e-81	295.00
IUUC-Loc-052377	Lepisosteus oculatus	50.96	1.00e-102	367.00
IUUC-Mcc-055344	Macaca mulatta	42.68	4.00e-67	249.00
IUUC-Meu-055950	Macropus eugenii	41.15	5.00e-60	225.00
IUUC-Mga-060049	Meleagris gallopavo	64.46	2.00e-45	176.00
IUUC-Mmr-061431	Microcebus murinus	40.46	8.00e-68	251.00
IUUC-Mdo-062016	Monodelphis domestica	42.53	4.00e-61	230.00
IUUC-Mmu-063368	Mus musculus	39.96	5.00e-80	292.00
IUUC-Mpu-066449	Mustela putorius furo	39.18	8.00e-70	258.00
IUUC-Mlu-067588	Myotis lucifugus	40.84	2.00e-60	226.00
IUUC-Nle-069192	Nomascus leucogenys	40.00	5.00e-67	248.00
IUUC-Opr-070300	Ochotona princeps	33.47	2.00e-54	207.00
IUUC-Ont-071998	Oreochromis niloticus	76.88	3.00e-175	608.00
IUUC-Oan-073610	Ornithorhynchus anatinus	57.14	5.00e-47	181.00
IUUC-Ocu-074603	Oryctolagus cuniculus	40.21	5.00e-68	252.00
IUUC-Ola-086681	Oryzias latipes	65.54	2.00e-158	552.00
IUUC-Oga-088183	Otolemur garnettii	40.46	1.00e-71	263.00
IUUC-Oar-090255	Ovis aries	64.29	4.00e-38	152.00
IUUC-Ptr-091029	Pan troglodytes	39.96	2.00e-63	236.00
IUUC-Pan-092241	Papio anubis	42.38	2.00e-67	249.00
IUUC-Psi-093506	Pelodiscus sinensis	60.24	5.00e-26	112.00
IUUC-Pma-094579	Petromyzon marinus	54.44	3.00e-25	109.00
IUUC-Pab-098530	Pongo abelii	40.00	8.00e-64	238.00
IUUC-Pca-100939	Procavia capensis	33.62	5.00e-38	152.00
IUUC-Pva-103148	Pteropus vampyrus	61.46	1.00e-32	134.00
IUUC-Rno-105338	Rattus norvegicus	39.79	8.00e-81	294.00
IUUC-Sha-107187	Sarcophilus harrisii	63.47	6.00e-45	174.00
IUUC-Sar-113356	Sorex araneus	37.93	2.00e-46	180.00
IUUC-Ssc-116290	Sus scrofa	40.79	3.00e-74	272.00
IUUC-Tru-117910	Takifugu rubripes	72.61	1.00e-164	573.00
IUUC-Tsy-119329	Tarsius syrichta	61.27	8.00e-35	141.00
IUUC-Tni-120521	Tetraodon nigroviridis	69.54	1.00e-149	523.00
IUUC-Ttr-128997	Tursiops truncatus	34.87	8.00e-54	204.00
IUUC-Vpa-130154	Vicugna pacos	37.27	1.00e-64	241.00
IUUC-Xtr-132805	Xenopus tropicalis	42.07	3.00e-70	259.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence