iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Dme-031740

UUCD1 version

UUC-DrM-00027

Ensembl Protein ID

FBpp0086011

UniProt Accession

Q9V853; Q9U3W2; SMUF1_DROME

Genbank Protein ID

AAL09691.1; AAM09646.1; AAF21125.1; AAF57824.3; AAX33558.1

Protein Name

E3 ubiquitin-protein ligase Smurf1; HECT-type E3 ubiquitin transferase Smurf1; Lethal with a checkpoint kinase protein; SMAD ubiquitination regulatory factor 1 homolog

Genbank Nucleotide ID

AF416571; AF464851; AF216521; AE013599; BT021410

Gene Name

lack; Smurf; Smurf1; CG4943

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
FBgn0029006	FBtr0086833	FBpp0086011

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
DNA & RNA Element
microRNA	RAID2
Protein-protein Interaction
IID	Mentha
Post-translational Modifications (PTMs)
dbPAF	dbPTM	Phospho.ELM	UniProt
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/HECT	HECT	16925950; 12754252

Classification

Family	E-value	Score	Start	End
E3 activity/HECT	2.60e-120	401.9	724	1060

Active Site

Position(s)	Description	Evidence
1029	Glycyl thioester intermediate	N/A

Domain Profile

E3 activity/HECT

   S: 2    ksdlkkrlevefvgEegldlgGvtreflallskelqnpdlglflysendketlqpnpdsglknpehlklFeflGrllgkalqdgrlldlplsksfy 97
           ++d++krl+v+f+gEegld+gGv+re+l+lls+e+ np++glf+ys +d++tlq+npdsg+ np+hl++F+f+Gr lg+a+++g++ld  ++++fy
   Q: 724  AKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGV-NPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFY 818
           689*********************************************************9.89******************************** PP
   S: 98   klllgkklsledleevdpelykslkeikenaesdieelgldltfsvseeilgetkeeeLkpgGenipVtneNkeeYvklvveyrlkegikeqveaf 193
           k+ll+k+++l d+e vdp+l++sl++++e+   +i+ + ++ tfsv+++++g   ++eLkpgG++ipVt+eNk+eYvkl+v+yr+ +gi++q+ a+
   Q: 819  KQLLNKPITLGDIEGVDPDLHRSLTWMLES---NISGI-IESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLAL 910
           ****************************88...99999.9******************************************************** PP
   S: 194  reGfneviplellslFteeelellicGeeeidvedlkketkykggytedspaiqwFwevleefsseerkafLqFvTgssrlPlgGfaelk...... 283
           ++Gf e+ip +ll+ F+e+elel+i G ++idv+d++++t+ k+ +t++  ++ wFw+v+e+ sse r+++LqFvTgssr+Pl+Gf++l+      
   Q: 911  QKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKH-CTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQgstgav 1005
           ******************************************99.999999999****************************************** PP
   S: 284  .prlfvirk..dasddrlPssnTCfnyLkLPeYsskeilkekLlyAieetkgFel 335
            prlf+i+   d  +++lP+++TCfn+++LP+Y++++ l +kL+ A+eet gF +
   Q: 1006 gPRLFTIHLtaDVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAV 1060
           *********877777**************************************76 PP

Organism

Drosophila melanogaster

Functional Description
(View)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Down-regulates Dpp signaling after gastrulation by promoting MAD ubiquitination and subsequent degradation.

Protein Sequence
(Fasta)

MNKLDYPRRN GTHKVRITIL CARNLARKDL FRLPDPFAKV QVDGTGQVYS TEISKSSLDP 60
KWNAHYDLFL GIGDAITITV WNQRKIHKGS GFLGCVRIPA FNIQSLKGAG FQRLDLGKLS 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

TGCCACCACT AATAAAAATG GCGTTCGAAG TTGTAGTTAA ATTTTTATGG GAATCCGTAA 60
AACAATAGCG CGTGAGGCCG CGGCAGCATA ATTCGCGCAC TATCAAGCGT TCCGTGGACG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0217--Developmental protein
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat
KW-0808--Transferase
KW-0833--Ubl conjugation pathway

Interpro

IPR000008--C2_dom
IPR000569--HECT_dom
IPR001202--WW_dom

PROSITE

PS50004--C2
PS50237--HECT
PS01159--WW_DOMAIN_1
PS50020--WW_DOMAIN_2

Pfam

PF00168--C2
PF00632--HECT
PF00397--WW

SMART

SM00239--C2
SM00119--HECTc
SM00456--WW

Gene Ontology

GO:0005737--C:cytoplasm
GO:0000151--C:ubiquitin ligase complex
GO:0004842--F:ubiquitin-protein transferase activity
GO:0008101--P:decapentaplegic signaling pathway
GO:0001700--P:embryonic development via the syncytial blastoderm
GO:0048619--P:embryonic hindgut morphogenesis
GO:0007293--P:germarium-derived egg chamber formation
GO:0030514--P:negative regulation of BMP signaling pathway
GO:0010629--P:negative regulation of gene expression
GO:0042787--P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0040008--P:regulation of growth

KEGG

dme:Dmel_CG4943

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001305	Ailuropoda melanoleuca	60.00	2.00e-176	612.00
IUUC-Apl-004155	Anas platyrhynchos	60.95	0.00e+00	702.00
IUUC-Aca-005358	Anolis carolinensis	60.28	1.00e-176	612.00
IUUC-Ame-010901	Astyanax mexicanus	72.54	1.00e-176	612.00
IUUC-Bta-013399	Bos taurus	62.77	0.00e+00	707.00
IUUC-Cja-018985	Callithrix jacchus	72.28	1.00e-176	612.00
IUUC-Cfa-020568	Canis familiaris	72.28	5.00e-176	610.00
IUUC-Cpo-022531	Cavia porcellus	72.28	1.00e-176	612.00
IUUC-Csa-024145	Chlorocebus sabaeus	59.76	3.00e-176	610.00
IUUC-Cho-024804	Choloepus hoffmanni	71.88	2.00e-138	485.00
IUUC-Cin-025126	Ciona intestinalis	60.88	0.00e+00	652.00
IUUC-Csv-026339	Ciona savignyi	70.10	2.00e-169	588.00
IUUC-Dre-028341	Danio rerio	60.56	4.00e-179	620.00
IUUC-Dno-029534	Dasypus novemcinctus	59.80	8.00e-176	609.00
IUUC-Ete-032715	Echinops telfairi	57.85	0.00e+00	637.00
IUUC-Eca-034125	Equus caballus	72.02	4.00e-176	610.00
IUUC-Eeu-034705	Erinaceus europaeus	71.76	3.00e-174	604.00
IUUC-Fca-035905	Felis catus	59.96	2.00e-178	618.00
IUUC-Fal-037275	Ficedula albicollis	59.61	7.00e-176	610.00
IUUC-Gmo-039486	Gadus morhua	66.23	4.00e-156	544.00
IUUC-Gga-040218	Gallus gallus	61.79	0.00e+00	707.00
IUUC-Gac-041707	Gasterosteus aculeatus	71.54	3.00e-175	607.00
IUUC-Ggo-044449	Gorilla gorilla	71.91	1.00e-173	601.00
IUUC-Hsa-045878	Homo sapiens	71.11	7.00e-177	613.00
IUUC-Itr-048317	Ictidomys tridecemlineatus	72.28	2.00e-176	612.00
IUUC-Lch-050026	Latimeria chalumnae	71.65	1.00e-173	602.00
IUUC-Loc-052628	Lepisosteus oculatus	72.54	5.00e-176	610.00
IUUC-Laf-053753	Loxodonta africana	60.00	2.00e-176	611.00
IUUC-Mcc-055479	Macaca mulatta	60.36	2.00e-179	622.00
IUUC-Meu-056436	Macropus eugenii	61.54	4.00e-146	511.00
IUUC-Mga-059481	Meleagris gallopavo	72.42	2.00e-175	608.00
IUUC-Mmr-061499	Microcebus murinus	71.11	7.00e-177	612.00
IUUC-Mdo-063005	Monodelphis domestica	62.66	0.00e+00	701.00
IUUC-Mmu-063634	Mus musculus	60.44	4.00e-179	620.00
IUUC-Mpu-065898	Mustela putorius furo	60.16	2.00e-178	618.00
IUUC-Mlu-067584	Myotis lucifugus	72.02	1.00e-175	608.00
IUUC-Nle-069033	Nomascus leucogenys	71.11	1.00e-176	612.00
IUUC-Opr-070591	Ochotona princeps	54.18	3.00e-152	531.00
IUUC-Ont-072265	Oreochromis niloticus	72.28	4.00e-176	610.00
IUUC-Oan-073621	Ornithorhynchus anatinus	60.16	1.00e-177	615.00
IUUC-Ocu-073960	Oryctolagus cuniculus	72.54	6.00e-176	610.00
IUUC-Ola-086597	Oryzias latipes	60.84	0.00e+00	709.00
IUUC-Oga-088579	Otolemur garnettii	71.11	8.00e-177	613.00
IUUC-Oar-090396	Ovis aries	71.50	7.00e-165	573.00
IUUC-Ptr-091624	Pan troglodytes	71.11	7.00e-177	613.00
IUUC-Pan-092479	Papio anubis	71.11	1.00e-176	612.00
IUUC-Psi-094074	Pelodiscus sinensis	63.16	0.00e+00	714.00
IUUC-Pma-094354	Petromyzon marinus	63.59	5.00e-144	504.00
IUUC-Ppa-095250	Physcomitrella patens	44.29	1.00e-81	296.00
IUUC-Pfo-096071	Poecilia formosa	71.50	3.00e-177	614.00
IUUC-Pab-098682	Pongo abelii	59.80	1.00e-175	609.00
IUUC-Pca-100516	Procavia capensis	65.23	8.00e-135	473.00
IUUC-Pva-102754	Pteropus vampyrus	58.42	9.00e-167	580.00
IUUC-Rno-105396	Rattus norvegicus	60.44	3.00e-179	621.00
IUUC-Sha-107216	Sarcophilus harrisii	60.03	0.00e+00	690.00
IUUC-Sar-113250	Sorex araneus	69.85	7.00e-172	596.00
IUUC-Ssc-116413	Sus scrofa	59.96	3.00e-169	587.00
IUUC-Tgu-116723	Taeniopygia guttata	60.31	0.00e+00	706.00
IUUC-Tru-117746	Takifugu rubripes	60.91	0.00e+00	707.00
IUUC-Tsy-119455	Tarsius syrichta	71.95	1.00e-159	555.00
IUUC-Tni-120037	Tetraodon nigroviridis	71.50	1.00e-173	602.00
IUUC-Tbe-127879	Tupaia belangeri	64.77	3.00e-152	531.00
IUUC-Ttr-128423	Tursiops truncatus	72.02	5.00e-167	580.00
IUUC-Vpa-130591	Vicugna pacos	65.21	9.00e-150	523.00
IUUC-Xtr-132237	Xenopus tropicalis	70.30	4.00e-176	610.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence