iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Sce-106318

UUCD1 version

UUC-SaC-00079

Ensembl Protein ID

YMR231W

UniProt Accession

P12868; D6W057; PEP5_YEAST

Genbank Protein ID

CAA38348.1; CAA33413.1; CAA90202.1; DAA10131.1

Protein Name

E3 ubiquitin-protein ligase PEP5; Carboxypeptidase Y-deficient protein 5; Histone E3 ligase PEP5; RING-type E3 ubiquitin transferase PEP5; Vacuolar biogenesis protein END1; Vacuolar morphogenesis protein 1; Vacuolar protein sorting-associated protein 11; Vacuolar protein-targeting protein 11

Genbank Nucleotide ID

X54466; X15355; Z49939; BK006946

Gene Name

PEP5; END1; VAM1; VPL9; VPS11; VPT11; YMR231W; YM9959.13

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
YMR231W	YMR231W	YMR231W

Annotation

mRNA Expression
GEO	FFGED
Protein-protein Interaction
IID	iRefIndex	HINT	Mentha
Post-translational Modifications (PTMs)
dbPAF	dbPTM	BioGRID
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	Identity	E-Value	Score	Source Orthology
E3 activity/RING/RING	36.11	2.70e-01	29.30	IUUC-Ots-000053

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Organism

Saccharomyces cerevisiae

Functional Description
(View)

Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. It is required for gluconeogenic growth of yeast. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion (PubMed:3062374, PubMed:10978279, PubMed:10944212, PubMed:16601699). Acts as component of the CORVET complex that is required for transport between endosome and vacuole. CORVET is an effector for the endosomal Rab GTPase VPS21 (PubMed:17488625). Probable ubiquitin-protein ligase involved in the degradation-related ubiquitination of histones. Contributes to the post-translational regulation of histone protein levels by polyubiquitination of excess histones for subsequent degradation (PubMed:22570702).

Protein Sequence
(Fasta)

MSLSSWRQFQ LFENIPIRDP NFGGDSLLYS DPTLCAATIV DPQTLIIAVN SNIIKVVKLN 60
QSQVIHEFQS FPHDFQITFL KVINGEFLVA LAESIGKPSL IRVYKLEKLP NREQLYHSQV 120

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Nucleotide Sequence
(Fasta)

ATGTCCCTGA GCTCCTGGAG GCAATTCCAG CTTTTCGAGA ATATTCCCAT AAGAGATCCT 60
AATTTTGGAG GAGATTCCCT ATTGTACTCG GACCCAACAC TTTGCGCAGC TACAATAGTA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0472--Membrane
KW-0479--Metal-binding
KW-0653--Protein transport
KW-1185--Reference proteome
KW-0808--Transferase
KW-0813--Transport
KW-0833--Ubl conjugation pathway
KW-0926--Vacuole
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR000547--Clathrin_H-chain/VPS_repeat
IPR011990--TPR-like_helical_dom
IPR016528--VPS11
IPR024763--VPS11_C
IPR015943--WD40/YVTN_repeat-like_dom
IPR017986--WD40_repeat_dom
IPR001965--Znf_PHD
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD

PROSITE

PS50236--CHCR
PS50089--ZF_RING_2

Pfam

PF12451--VPS11_C

SMART

SM00249--PHD
SM00184--RING

Gene Ontology

GO:0033263--C:CORVET complex
GO:0005829--C:cytosol
GO:0000329--C:fungal-type vacuole membrane
GO:0030897--C:HOPS complex
GO:0030674--F:protein binding, bridging
GO:0004842--F:ubiquitin-protein transferase activity
GO:0008270--F:zinc ion binding
GO:0007032--P:endosome organization
GO:0006895--P:Golgi to endosome transport
GO:0036205--P:histone catabolic process
GO:0016574--P:histone ubiquitination
GO:0006886--P:intracellular protein transport
GO:0045324--P:late endosome to vacuole transport
GO:0007040--P:lysosome organization
GO:0035542--P:regulation of SNARE complex assembly
GO:0032889--P:regulation of vacuole fusion, non-autophagic
GO:0042144--P:vacuole fusion, non-autophagic
GO:0006904--P:vesicle docking involved in exocytosis
GO:0099022--P:vesicle tethering

KEGG

sce:YMR231W

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Atr-003088	Amborella trichopoda	47.62	2.00e-04	38.50
IUUC-Apl-003478	Anas platyrhynchos	25.35	4.90e-02	33.10
IUUC-Dme-031777	Drosophila melanogaster	31.48	6.00e-02	33.90
IUUC-Lpe-051547	Leersia perrieri	42.31	1.00e-03	37.00
IUUC-Mla-059167	Melampsora laricipopulina	33.33	1.00e-03	36.20
IUUC-Mga-059943	Meleagris gallopavo	25.35	5.00e-02	33.50
IUUC-Opu-083349	Oryza punctata	36.51	1.80e-02	33.10
IUUC-Pma-094295	Petromyzon marinus	26.87	7.00e-04	35.40
IUUC-Smo-109244	Selaginella moellendorffii	48.72	1.00e-05	39.30
IUUC-Sly-111278	Solanum lycopersicum	32.84	4.00e-05	40.40
IUUC-Stu-112834	Solanum tuberosum	32.43	2.00e-05	41.20
IUUC-Sbi-114365	Sorghum bicolor	34.09	2.00e-03	34.30

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence