iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

UUCD2 ID

IUUC-Mmu-064034

UUCD1 version

UUC-MuM-00079

Ensembl Protein ID

ENSMUSP00000090177.2

UniProt Accession

A2A5Z6; A2A5Z7; Q5IRE6; SMUF2_MOUSE

Genbank Protein ID

AAV87906.1; CAM16223.1; CAM16224.1; EDL34316.1; AAI38787.1; AAI38789.1

Protein Name

E3 ubiquitin-protein ligase SMURF2; HECT-type E3 ubiquitin transferase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2

Genbank Nucleotide ID

AY685230; AL593847; AL593847; CH466558; BC138786; BC138788

Gene Name

Smurf2

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMUSG00000018363.16	ENSMUST00000103067.9	ENSMUSP00000099356.3
ENSMUSG00000018363.16	ENSMUST00000092517.8	ENSMUSP00000090177.2

Annotation

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	miRTarBase	microRNA	TRANSFAC
RepTar	miRNAMap	RAID2
Protein-protein Interaction
IID	iRefIndex	HINT
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	mUbiSiDa
Protein Expression/Proteomics
GPMDB

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E3 activity/HECT	8.50e-130	434.2	415	747

Active Site

Position(s)	Description	Evidence
716	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00104}

Domain Profile

E3 activity/HECT

   S: 2    ksdlkkrlevefvgEegldlgGvtreflallskelqnpdlglflysendketlqpnpdsglknpehlklFeflGrllgkalqdgrlldl 90
           ++dl krl+++f+gEegld+gGv+re+l+lls+e+ np++glf+ys +d +tlq+npds + npehl++F+f+Gr++g+a+++g+++d 
   Q: 415  PKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAV-NPEHLSYFHFVGRIMGMAVFHGHYIDG 502
           678999*****************************************************98.89************************* PP
   S: 91   plsksfyklllgkklsledleevdpelykslkeikenaesdieelgldltfsvseeilgetkeeeLkpgGenipVtneNkeeYvklvve 179
            ++ +fyk+llgk+++l+d+e vdp+l++sl +i+en   di+   ld tf+v+++ +ge+ ++eLkp+G++ipVt+eNk+eYv+l+v+
   Q: 503  GFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILEN---DITGV-LDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVN 587
           ***********************************99...9***8.******************************************* PP
   S: 180  yrlkegikeqveafreGfneviplellslFteeelellicGeeeidvedlkketkykggytedspaiqwFwevleefsseerkafLqFv 268
           +r+ +gi+ q+ a+++Gfnevip++ll+ F+e+elel+icG  +idv+d+k +t+ k+ +t+ds++++wFw+++e f++e r+++LqFv
   Q: 588  WRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKH-CTPDSNVVKWFWKAVEFFDEERRARLLQFV 675
           ********************************************************99.****************************** PP
   S: 269  TgssrlPlgGfaelk....prlfvirk.dasddrlPssnTCfnyLkLPeYsskeilkekLlyAieetkgFel 335
           Tgssr+Pl+Gf++l+    prlf+i++ da +++lP+++TCfn++++P+Y+s+e+l+ekLl+Aieet gF +
   Q: 676  TGSSRVPLQGFKALQgaagPRLFTIHQiDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAV 747
           **********************************************************************76 PP

Organism

Mus musculus

Functional Description
(View)

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

Protein Sequence
(Fasta)

MSNPGGRRNG PVKLRLTVLC AKNLVKKDFF RLPDPFAKVV VDGSGQCHST DTVKNTLDPK 60
WNQHYDLYIG KSDSVTISVW NHKKIHKKQG AGFLGCVRLL SNAINRLKDT GYQRLDLCKL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGCTGCGGCC CCAGGACCGG GTTCTCTCAG ACAAGAGGGC GGGCGGAGGA GGAAGCGGCC 60
GAGCACCCGC CCGCCTCAGC AGACTTCGCA CCGTCCGCTC CCAGGCCTCG CGGCACCGAG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0025--Alternative splicing
KW-1003--Cell membrane
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-1017--Isopeptide bond
KW-0472--Membrane
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0677--Repeat
KW-0808--Transferase
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR000008--C2_dom
IPR000569--HECT_dom
IPR001202--WW_dom

PROSITE

PS50004--C2
PS50237--HECT
PS01159--WW_DOMAIN_1
PS50020--WW_DOMAIN_2

Pfam

PF00168--C2
PF00632--HECT
PF00397--WW

SMART

SM00239--C2
SM00119--HECTc
SM00456--WW

Gene Ontology

GO:0005829--C:cytosol
GO:0045121--C:membrane raft
GO:0016607--C:nuclear speck
GO:0005886--C:plasma membrane
GO:0000151--C:ubiquitin ligase complex
GO:0042802--F:identical protein binding
GO:0046332--F:SMAD binding
GO:0061630--F:ubiquitin protein ligase activity
GO:0004842--F:ubiquitin-protein transferase activity
GO:0030512--P:negative regulation of transforming growth factor beta receptor signaling pathway
GO:1901165--P:positive regulation of trophoblast cell migration
GO:0006511--P:ubiquitin-dependent protein catabolic process
GO:0030579--P:ubiquitin-dependent SMAD protein catabolic process

KEGG

mmu:66313

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001305	Ailuropoda melanoleuca	98.78	0.00e+00	1491.00
IUUC-Apl-003513	Anas platyrhynchos	97.41	0.00e+00	1474.00
IUUC-Aca-005358	Anolis carolinensis	96.95	0.00e+00	1498.00
IUUC-Ame-011320	Astyanax mexicanus	87.99	0.00e+00	1390.00
IUUC-Bta-013399	Bos taurus	99.18	0.00e+00	1485.00
IUUC-Cja-019355	Callithrix jacchus	98.12	0.00e+00	1493.00
IUUC-Cpo-022200	Cavia porcellus	99.18	0.00e+00	1492.00
IUUC-Cin-025126	Ciona intestinalis	65.66	0.00e+00	972.00
IUUC-Csv-026339	Ciona savignyi	64.91	0.00e+00	1007.00
IUUC-Dre-027650	Danio rerio	90.72	0.00e+00	1433.00
IUUC-Dno-029534	Dasypus novemcinctus	99.20	0.00e+00	1524.00
IUUC-Ete-032715	Echinops telfairi	94.38	0.00e+00	1402.00
IUUC-Eca-033580	Equus caballus	99.45	0.00e+00	1496.00
IUUC-Eeu-034945	Erinaceus europaeus	97.27	0.00e+00	1385.00
IUUC-Fal-037275	Ficedula albicollis	96.36	0.00e+00	1462.00
IUUC-Gga-041060	Gallus gallus	94.02	0.00e+00	1428.00
IUUC-Gac-041295	Gasterosteus aculeatus	91.26	0.00e+00	1395.00
IUUC-Hsa-046726	Homo sapiens	99.20	0.00e+00	1523.00
IUUC-Lch-050026	Latimeria chalumnae	95.23	0.00e+00	1461.00
IUUC-Loc-051760	Lepisosteus oculatus	87.73	0.00e+00	1376.00
IUUC-Laf-053753	Loxodonta africana	99.32	0.00e+00	1493.00
IUUC-Mcc-054815	Macaca mulatta	99.20	0.00e+00	1524.00
IUUC-Meu-056436	Macropus eugenii	76.40	0.00e+00	1107.00
IUUC-Mmr-060806	Microcebus murinus	99.18	0.00e+00	1494.00
IUUC-Mdo-062805	Monodelphis domestica	97.73	0.00e+00	1498.00
IUUC-Mpu-066762	Mustela putorius furo	98.78	0.00e+00	1491.00
IUUC-Mlu-066981	Myotis lucifugus	99.32	0.00e+00	1492.00
IUUC-Nle-069804	Nomascus leucogenys	99.19	0.00e+00	1518.00
IUUC-Opr-070591	Ochotona princeps	94.25	0.00e+00	1400.00
IUUC-Ont-072243	Oreochromis niloticus	88.92	0.00e+00	1409.00
IUUC-Oan-072699	Ornithorhynchus anatinus	95.71	0.00e+00	1497.00
IUUC-Ocu-074913	Oryctolagus cuniculus	99.47	0.00e+00	1527.00
IUUC-Ola-086597	Oryzias latipes	85.36	0.00e+00	1280.00
IUUC-Oga-089116	Otolemur garnettii	95.63	0.00e+00	1428.00
IUUC-Oar-089366	Ovis aries	98.91	0.00e+00	1488.00
IUUC-Ptr-091382	Pan troglodytes	99.20	0.00e+00	1523.00
IUUC-Psi-093298	Pelodiscus sinensis	97.68	0.00e+00	1455.00
IUUC-Pma-094354	Petromyzon marinus	74.08	0.00e+00	1125.00
IUUC-Pfo-096071	Poecilia formosa	89.11	0.00e+00	1415.00
IUUC-Pab-098682	Pongo abelii	99.20	0.00e+00	1524.00
IUUC-Pca-100516	Procavia capensis	90.12	0.00e+00	636.00
IUUC-Pva-102754	Pteropus vampyrus	98.22	0.00e+00	1457.00
IUUC-Rno-105600	Rattus norvegicus	99.37	0.00e+00	1293.00
IUUC-Sha-107144	Sarcophilus harrisii	97.36	0.00e+00	1357.00
IUUC-Spo-108171	Schizosaccharomyces pombe	40.37	4.00e-164	572.00
IUUC-Sar-113122	Sorex araneus	88.08	0.00e+00	1276.00
IUUC-Tgu-117112	Taeniopygia guttata	96.68	0.00e+00	1491.00
IUUC-Tru-118497	Takifugu rubripes	89.34	0.00e+00	1369.00
IUUC-Tni-120781	Tetraodon nigroviridis	89.92	0.00e+00	1180.00
IUUC-Tme-126897	Tuber melanosporum	39.42	1.00e-153	536.00
IUUC-Tbe-127371	Tupaia belangeri	88.99	0.00e+00	1216.00
IUUC-Ttr-129305	Tursiops truncatus	91.25	0.00e+00	1280.00
IUUC-Vpa-130591	Vicugna pacos	88.90	0.00e+00	1295.00
IUUC-Xtr-132979	Xenopus tropicalis	76.79	0.00e+00	1103.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence