iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSG00000135093.12	ENST00000257548.9	ENSP00000257548.5
ENSG00000135093.12	ENST00000377883.7	ENSP00000473942.1
ENSG00000135093.12	ENST00000536393.5	ENSP00000438829.1
ENSG00000135093.12	ENST00000539121.1	ENSP00000440837.1

Annotation

Cancer Mutation
TCGA	ICGC	COSMIC	CGAP
IntOGen
Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	TCGA	ICGC
COSMIC	THPA	HPM
DNA & RNA Element
UTRdb	AREsite	circBase	circRNADb
CircNet	miRTarBase	microRNA	TRANSFAC
TargetScan	RepTar	miRNAMap	SomamiR
miRcode	RAID2
Protein-protein Interaction
IID	PINA	HINT	Mentha
InWeb_IM
Disease-associated Information
OMIM
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	BioGRID
DNA Methylation
TCGA	ICGC	COSMIC
Protein Expression/Proteomics
THPA	HPM	GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
DUB/USP	USP	14715245

Classification

Family	E-value	Score	Start	End
DUB/USP	4.50e-71	241.4	69	501

Active Site

Position(s)	Description	Evidence
77	Nucleophile	{ECO:0000269\|PubMed:18287522, ECO:0000269\|PubMed:24896179, ECO:0000269\|PubMed:25621951}
452	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 2    GlkNlgNtCYmNSvLQcLsnipelrelllekkkeklneenpkepkgellkslqklfeelw...kskkkavsprellkalkkeaeefsgyeqq 90
           Gl NlgNtC+mNS+LQ Ls++p + + l e +++  ++++++ +++ l+ +l +l+++l     +++++ + + ll++l+++  ++s++e+q
   Q: 69   GLVNLGNTCFMNSLLQGLSACPAFIRWLEEFTSQYSRDQKEPPSHQYLSLTLLHLLKALScqeVTDDEVLDASCLLDVLRMYRWQISSFEEQ 160
           9*****************************66666666666788899************888899999************************ PP
   S: 91   DaqEflaflldqleeelkkeknkpkeeeeeedkselsde..........aeekkkkknkslisdlFqgqlksqikclecdnvs.sreepfld 171
           Da+E+++++ ++le+e    ++ +++++++ ++ e+++e          + + ++++n+   ++ F+g+l+s+++c++c+++s +r+++f +
   Q: 161  DAHELFHVITSSLEDER--DRQPRVTHLFDVHSLEQQSEitpkqitcrtRGSPHPTSNHWKSQHPFHGRLTSNMVCKHCEHQSpVRFDTFDS 250
           ****************9..668888889998888888888999999999999**************************************** PP
   S: 172  lslpvpgkeetkkeslqdcLesftkeeelegenkyycekck...............kkqeaekklklkklPevLilhLkRfsfseeqksrek 248
           lsl++p  +  ++++l++cL++f + e++++   + c++c+               ++++ +k+lkl klP++L++hL+R s+s++ + + k
   Q: 251  LSLSIPAATWGHPLTLDHCLHHFISSESVRD---VVCDNCTkieakgtlngekvehQRTTFVKQLKLGKLPQCLCIHLQRLSWSSH-GTPLK 338
           *****************************97...9******8888888888888777777***********************998.8888* PP
   S: 249  inkkvefpleLdlspfcts..........peekqke........................................................ 274
            +++v+f++ L ++ +  +          p     +                                                        
   Q: 339  RHEHVQFNEFLMMDIYKYHllghkpsqhnP-----Klnknpgptlelqdgpgaptpvlnqpgapktqifmngacspsllptlsapmpfplpv 425
           ********8877775444444556555551.....144455555666666666666666666666666666677777777777777777777 PP
   S: 275  .......kkkYeLiavvvHsGslssGHYvayvkn.........ekgkWllfdDskVtevkeeevleetgstaYvlfYkr 337
                  ++ ++L+avvvH+G ++sGH+v+y ++         ++++Wl ++D++V++ + +evl+   s+aY+lfY+r
   Q: 426  vpdysssTYLFRLMAVVVHHGDMHSGHFVTYRRSppsarnplsTSNQWLWVSDDTVRKASLQEVLS---SSAYLLFYER 501
           77777669999***********************9999988888999*******************...********98 PP

Organism

Homo sapiens

Functional Description
(View)

Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PARK2): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:18287522, PubMed:24896179, PubMed:25527291, PubMed:25621951). Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate to mitophagic signaling (PubMed:25621951). Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (PubMed:25527291). Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity).

Protein Sequence
(Fasta)

MLSSRAEAAM TAADRAIQRF LRTGAAVRYK VMKNWGVIGG IAAALAAGIY VIWGPITERK 60
KRRKGLVPGL VNLGNTCFMN SLLQGLSACP AFIRWLEEFT SQYSRDQKEP PSHQYLSLTL 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATCCCCATTG TTGCATAGCT GGGAAAGGGA GCTGGACAAC CTGGTACCTT TTCGATTATG 60
GATTCAAATG TCTCTGAAGG TTTTTGCTTT AATTACCCCT TTTCCCTCAA TCCTGGTGTC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0378--Hydrolase
KW-1017--Isopeptide bond
KW-0472--Membrane
KW-0496--Mitochondrion
KW-1000--Mitochondrion outer membrane
KW-0621--Polymorphism
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0812--Transmembrane
KW-1133--Transmembrane helix
KW-0832--Ubl conjugation
KW-0833--Ubl conjugation pathway

Interpro

IPR001394--Peptidase_C19_UCH
IPR018200--USP_CS
IPR028889--USP_dom

PROSITE

PS00972--USP_1
PS00973--USP_2
PS50235--USP_3

Pfam

PF00443--UCH

Gene Ontology

GO:0016021--C:integral component of membrane
GO:0005741--C:mitochondrial outer membrane
GO:0005739--C:mitochondrion
GO:0004197--F:cysteine-type endopeptidase activity
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0036459--F:thiol-dependent ubiquitinyl hydrolase activity
GO:0008053--P:mitochondrial fusion
GO:0000422--P:mitophagy
GO:0016579--P:protein deubiquitination
GO:0035871--P:protein K11-linked deubiquitination
GO:0044313--P:protein K6-linked deubiquitination
GO:0006511--P:ubiquitin-dependent protein catabolic process

KEGG

hsa:84749

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000204	Aegilops tauschii	29.04	2.00e-17	83.60
IUUC-Aml-001733	Ailuropoda melanoleuca	93.08	0.00e+00	875.00
IUUC-Atr-002510	Amborella trichopoda	24.28	2.00e-20	94.40
IUUC-Apl-003645	Anas platyrhynchos	71.48	3.00e-107	381.00
IUUC-Aca-004583	Anolis carolinensis	68.42	1.00e-24	103.00
IUUC-Aly-005754	Arabidopsis lyrata	26.81	2.00e-22	100.00
IUUC-Ath-007622	Arabidopsis thaliana	26.54	4.00e-23	102.00
IUUC-Ame-010896	Astyanax mexicanus	62.92	5.00e-156	544.00
IUUC-Bta-012321	Bos taurus	91.49	0.00e+00	859.00
IUUC-Bdi-014795	Brachypodium distachyon	27.92	1.00e-18	87.80
IUUC-Bol-015637	Brassica oleracea	26.81	1.00e-23	104.00
IUUC-Bra-016801	Brassica rapa	24.53	4.00e-20	92.80
IUUC-Cel-018308	Caenorhabditis elegans	28.10	1.00e-37	150.00
IUUC-Cja-018826	Callithrix jacchus	98.07	0.00e+00	968.00
IUUC-Cfa-021206	Canis familiaris	90.96	0.00e+00	880.00
IUUC-Cpo-021343	Cavia porcellus	93.21	0.00e+00	865.00
IUUC-Csa-023419	Chlorocebus sabaeus	99.23	0.00e+00	976.00
IUUC-Cho-024475	Choloepus hoffmanni	89.82	1.00e-139	489.00
IUUC-Cgl-026621	Colletotrichum gloeosporioides	20.79	1.00e-11	65.10
IUUC-Cne-027061	Cryptococcus neoformans	24.71	8.00e-10	59.30
IUUC-Dre-027563	Danio rerio	59.88	5.00e-160	557.00
IUUC-Dno-029929	Dasypus novemcinctus	93.23	0.00e+00	910.00
IUUC-Dor-030166	Dipodomys ordii	83.56	0.00e+00	757.00
IUUC-Dme-031627	Drosophila melanogaster	35.29	1.00e-36	148.00
IUUC-Ete-032634	Echinops telfairi	80.85	0.00e+00	699.00
IUUC-Eca-033968	Equus caballus	94.08	0.00e+00	850.00
IUUC-Eeu-035279	Erinaceus europaeus	82.32	1.00e-58	221.00
IUUC-Fca-036525	Felis catus	93.81	0.00e+00	905.00
IUUC-Fal-036606	Ficedula albicollis	74.61	1.00e-160	559.00
IUUC-Gmo-038726	Gadus morhua	56.80	4.00e-143	501.00
IUUC-Gga-040718	Gallus gallus	74.94	1.00e-162	565.00
IUUC-Gac-042645	Gasterosteus aculeatus	57.65	7.00e-151	527.00
IUUC-Gma-042890	Glycine max	25.66	4.00e-26	112.00
IUUC-Ggo-045021	Gorilla gorilla	100.00	0.00e+00	984.00
IUUC-Itr-048392	Ictidomys tridecemlineatus	95.10	0.00e+00	869.00
IUUC-Lch-050662	Latimeria chalumnae	70.76	0.00e+00	629.00
IUUC-Lpe-051078	Leersia perrieri	26.48	5.00e-31	129.00
IUUC-Loc-052611	Lepisosteus oculatus	65.39	7.00e-180	623.00
IUUC-Lma-053048	Leptosphaeria maculans	20.51	5.00e-16	80.10
IUUC-Laf-053752	Loxodonta africana	92.65	0.00e+00	842.00
IUUC-Mcc-054892	Macaca mulatta	99.61	0.00e+00	979.00
IUUC-Meu-056673	Macropus eugenii	75.00	3.00e-164	570.00
IUUC-Mor-057143	Magnaporthe oryzae	20.99	8.00e-08	52.80
IUUC-Mtr-057976	Medicago truncatula	26.51	3.00e-32	133.00
IUUC-Mla-058970	Melampsora laricipopulina	23.34	6.00e-20	92.40
IUUC-Mga-059543	Meleagris gallopavo	76.60	0.00e+00	672.00
IUUC-Mmr-061534	Microcebus murinus	95.16	0.00e+00	910.00
IUUC-Mdo-062140	Monodelphis domestica	69.56	2.00e-167	582.00
IUUC-Mmu-063673	Mus musculus	89.75	0.00e+00	850.00
IUUC-Mpu-066328	Mustela putorius furo	92.46	0.00e+00	864.00
IUUC-Mlu-067938	Myotis lucifugus	93.23	0.00e+00	914.00
IUUC-Nle-069426	Nomascus leucogenys	99.10	0.00e+00	881.00
IUUC-Opr-070796	Ochotona princeps	85.60	1.00e-123	436.00
IUUC-Ont-072248	Oreochromis niloticus	57.52	3.00e-157	548.00
IUUC-Oan-072916	Ornithorhynchus anatinus	80.12	0.00e+00	772.00
IUUC-Ocu-074459	Oryctolagus cuniculus	87.95	0.00e+00	671.00
IUUC-Oba-075765	Oryza barthii	27.25	5.00e-30	125.00
IUUC-Obr-076005	Oryza brachyantha	27.03	1.00e-30	128.00
IUUC-Ogl-077916	Oryza glaberrima	27.50	1.00e-33	138.00
IUUC-Ogu-078263	Oryza glumaepatula	27.40	7.00e-31	128.00
IUUC-Oin-080096	Oryza indica	28.10	1.00e-24	107.00
IUUC-Ome-081928	Oryza meridionalis	27.45	5.00e-32	132.00
IUUC-Oni-082424	Oryza nivara	27.40	7.00e-31	128.00
IUUC-Oru-085117	Oryza rufipogon	27.40	7.00e-31	128.00
IUUC-Ola-087429	Oryzias latipes	57.45	2.00e-148	519.00
IUUC-Oga-087981	Otolemur garnettii	94.97	0.00e+00	900.00
IUUC-Oar-089927	Ovis aries	90.91	0.00e+00	853.00
IUUC-Ptr-090878	Pan troglodytes	100.00	0.00e+00	984.00
IUUC-Pan-092698	Papio anubis	99.61	0.00e+00	979.00
IUUC-Psi-093451	Pelodiscus sinensis	77.48	0.00e+00	725.00
IUUC-Pfo-096096	Poecilia formosa	58.17	8.00e-154	536.00
IUUC-Pab-098676	Pongo abelii	98.65	0.00e+00	961.00
IUUC-Pca-100418	Procavia capensis	96.55	4.00e-131	461.00
IUUC-Ppe-101898	Prunus persica	24.47	8.00e-25	108.00
IUUC-Pva-103018	Pteropus vampyrus	91.68	0.00e+00	881.00
IUUC-Pgr-103423	Puccinia graminis	26.36	2.00e-09	58.20
IUUC-Pte-104330	Pyrenophora teres	21.76	8.00e-15	75.90
IUUC-Pyt-104518	Pyrenophora triticirepentis	21.64	2.00e-16	81.30
IUUC-Rno-105708	Rattus norvegicus	88.78	0.00e+00	839.00
IUUC-Sha-106971	Sarcophilus harrisii	79.73	0.00e+00	784.00
IUUC-Sit-110442	Setaria italica	26.61	8.00e-28	118.00
IUUC-Sly-110928	Solanum lycopersicum	24.08	7.00e-16	78.60
IUUC-Stu-112223	Solanum tuberosum	25.07	3.00e-17	83.20
IUUC-Ssc-116397	Sus scrofa	91.10	0.00e+00	857.00
IUUC-Tgu-117446	Taeniopygia guttata	70.39	0.00e+00	645.00
IUUC-Tru-118268	Takifugu rubripes	59.08	4.00e-143	501.00
IUUC-Tsy-119484	Tarsius syrichta	92.13	9.00e-53	201.00
IUUC-Tni-119735	Tetraodon nigroviridis	53.32	6.00e-122	431.00
IUUC-Tca-121660	Theobroma cacao	24.48	5.00e-28	119.00
IUUC-Tae-125383	Triticum aestivum	27.89	2.00e-29	124.00
IUUC-Tur-126458	Triticum urartu	27.10	3.00e-26	113.00
IUUC-Tbe-127740	Tupaia belangeri	88.59	0.00e+00	811.00
IUUC-Ttr-129273	Tursiops truncatus	90.33	0.00e+00	852.00
IUUC-Vda-129789	Verticillium dahliae	22.84	2.00e-08	54.70
IUUC-Vvi-131177	Vitis vinifera	25.78	2.00e-27	117.00
IUUC-Xtr-132374	Xenopus tropicalis	66.47	2.00e-174	605.00
IUUC-Xma-133267	Xiphophorus maculatus	60.72	2.00e-157	548.00
IUUC-Yli-134571	Yarrowia lipolytica	23.53	5.00e-12	66.20

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence