iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress	GXD
DNA & RNA Element
AREsite	miRTarBase	microRNA	TRANSFAC
RepTar	miRNAMap	RAID2
Protein-protein Interaction
IID	iRefIndex	Mentha
Post-translational Modifications (PTMs)
CPLM	dbPAF	PhosphositePlus	dbPTM
Phospho.ELM	UniProt	PHOSIDA	mUbiSiDa
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
UBD/Alpha-Helix/UBA	UBA	10549293
ULD/UDP/UBQ/UBQ_PIM	UBQ_PIM	10807547; 24674348

Classification

Family	E-value	Score	Start	End
UBD/Alpha-Helix/UBA	3.80e-10	40.7	596	633
ULD/UDP/UBQ/UBQ_PIM	1.30e-23	83.9	33	106

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

UBD/Alpha-Helix/UBA

   S: 3    dedkleqlveMGF.dreealqaLraannnleaAveyLl 39
           ++++leql +MGF +re++lqaL a++++++aA+e+Ll
   Q: 596  FQQQLEQLNAMGFlNREANLQALIATGGDINAAIERLL 633
           799**********************************9 PP

ULD/UDP/UBQ/UBQ_PIM

   S: 1    mkltvktlkgkefelevseddtveelKekiakesgvppeqqkLIyaGkiLkDdttLselgikdgsvvhlvvkksk 75
           +k+tvkt+k+ke e+ v e++tv+++Ke+i+k+++ + +q+ LI+aGkiLkD++tL ++gi+dg +vhlv+k ++
   Q: 33   IKVTVKTPKEKE-EFAVPENSTVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLMQHGIHDGLTVHLVIKSQN 106
           69**********.***********************************************************986 PP

Organism

Mus musculus

Functional Description
(View)

Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome. Plays a role in the ERAD pathway via its interaction with ER-localized proteins FAF2/UBXD8 and HERPUD1 and may form a link between the polyubiquitinated ERAD substrates and the proteasome. Involved in the regulation of macroautophagy and autophagosome formation; required for maturation of autophagy-related protein LC3 from the cytosolic form LC3-I to the membrane-bound form LC3-II and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion. Negatively regulates the endocytosis of GPCR receptors: AVPR2 and ADRB2, by specifically reducing the rate at which receptor-arrestin complexes concentrate in clathrin-coated pits (CCPs) (By similarity). Links CD47 to vimentin-containing intermediate filaments of the cytoskeleton (PubMed:10549293).

Protein Sequence
(Fasta)

MAENGESSGP PRPSRGPAAA PGAASPPAEP KIIKVTVKTP KEKEEFAVPE NSTVQQFKEA 60
ISKRFKSQTD QLVLIFAGKI LKDQDTLMQH GIHDGLTVHL VIKSQNRPQG QATTQPSTTA 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

GGCAGCGGAG ACGGCCTGCA GGACCTGCTC TCTCAGCCCT CAGCCGAGGC CTACGCCGAG 60
CCGAGTGCGC AGCCGACGAC CGGGAGGAGC CGCAGCCTTC AACTCTGAGG TACTGTGATC 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0007--Acetylation
KW-0072--Autophagy
KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0968--Cytoplasmic vesicle
KW-0472--Membrane
KW-0539--Nucleus
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0677--Repeat

Interpro

IPR016024--ARM-type_fold
IPR006636--STI1_HS-bd
IPR015940--UBA
IPR009060--UBA-like
IPR015496--Ubiquilin
IPR029071--Ubiquitin-rel_dom
IPR000626--Ubiquitin_dom

PROSITE

PS50030--UBA
PS50053--UBIQUITIN_2

Pfam

PF00627--UBA
PF00240--ubiquitin

SMART

SM00727--STI1
SM00165--UBA
SM00213--UBQ

Gene Ontology

GO:0005776--C:autophagosome
GO:0005737--C:cytoplasm
GO:0031410--C:cytoplasmic vesicle
GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0005886--C:plasma membrane
GO:0006914--P:autophagy
GO:1900186--P:negative regulation of clathrin-dependent endocytosis
GO:1904021--P:negative regulation of G-protein coupled receptor internalization
GO:1903071--P:positive regulation of ER-associated ubiquitin-dependent protein catabolic process
GO:2000785--P:regulation of autophagosome assembly
GO:0016241--P:regulation of macroautophagy
GO:0030433--P:ubiquitin-dependent ERAD pathway

KEGG

mmu:54609

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Bta-013571	Bos taurus	90.75	2.00e-170	592.00
IUUC-Cre-022634	Chlamydomonas reinhardtii	48.15	3.00e-11	62.80
IUUC-Csa-023627	Chlorocebus sabaeus	89.68	8.00e-171	593.00
IUUC-Ete-033071	Echinops telfairi	85.71	2.00e-166	579.00
IUUC-Eeu-034727	Erinaceus europaeus	89.25	7.00e-169	587.00
IUUC-Fso-038204	Fusarium solani	43.66	9.00e-09	55.10
IUUC-Mor-057194	Magnaporthe oryzae	49.02	1.00e-07	51.20
IUUC-Ncr-068732	Neurospora crassa	48.00	1.00e-07	50.80
IUUC-Nle-069038	Nomascus leucogenys	89.68	2.00e-170	592.00
IUUC-Pca-100227	Procavia capensis	87.08	5.00e-88	318.00
IUUC-Pgr-103261	Puccinia graminis	53.33	2.00e-07	50.40
IUUC-Rno-105748	Rattus norvegicus	95.04	0.00e+00	630.00
IUUC-Vda-129991	Verticillium dahliae	46.30	3.00e-07	50.10

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence