iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

UUCD2 ID

IUUC-Mlu-067248

UUCD1 version

Ensembl Protein ID

UniProt Accession

Genbank Protein ID

Protein Name

Ubiquitin carboxyl-terminal hydrolase 16; Deubiquitinating enzyme 16; Ubiquitin thioesterase 16; Ubiquitin-specific-processing protease 16

Genbank Nucleotide ID

AAPE02043682

Gene Name

USP16

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSMLUG00000029343.1	ENSMLUT00000026082.1	ENSMLUP00000021214.1

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
DUB/USP	2.90e-94	316.1	194	813

Active Site

Position(s)	Description	Evidence
203	Nucleophile	ECO:0000256\|HAMAP-Rule:MF_03062}
749	Proton acceptor	ECO:0000256\|HAMAP-Rule:MF_03062}

Domain Profile

DUB/USP

   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrellle.........kkkeklneenp............kepkgellkslqklfeelwkskkkav 68
           +Gl+NlgNtC++N+v+Q Ls++p lrell e                  +p             ep g+l+ ++ ++++e+++++k+ v
   Q: 194  KGLSNLGNTCFFNAVMQNLSQTPVLRELLEEvkmsgtvvkI--------EPpdlaltepleihLEPPGPLTLAMSQFLSEMQETSKGIV 274
           5******************************7444444441........3356666666777799999********************* PP
   S: 69   sprellkalkkeaeefsgyeqqDaqEflaflldqleeelkkeknkpkeeeeeedkselsde.....aeekkkkknkslisdlFqgqlks 152
           +p+el+++++k+a++f+gy+qqD+qE+l +lld+++ee +++ +k + +++++++++l++e     ++++kk++ +s+++++F+g+l+s
   Q: 275  TPKELFSQVCKKAVHFEGYQQQDSQELLWYLLDGMREEEHQRVSKGVLKAFGNSTEKLDEElkskvKDYEKKESVPSFVDRIFGGELTS 363
           ***********************************************************99999999999******************* PP
   S: 153  qikclecdnvssreepfldlslpvpgkeetkke........................................................ 185
           +i+c ec++vs  +e+fldlslpv ++++ k++                                                        
   Q: 364  TIMCDECRTVSLVHESFLDLSLPVLDDQSGKESindknlkktmenedkdreeekdnytyikerndipsgiskhlqkkakkqakkqaknq 452
           ****************************99888******************************************************** PP
   S: 186  ......................................................................................... 185
                                                                                                    
   Q: 453  rrqqkiqgkvfhfndvcanhlednereaeksvqgevdiksdhisqeevvneeycvnqndlngqekviekdvdvrsvsvdndvevlsssp 541
           ***************************************************************************************** PP
   S: 186  .................................................................................slqdcLes 193
                                                                                            s+q+cL +
   Q: 542  tdcpgslnsacldaggggevdisrgfqnlhldaalhpddirleilngspapgttlyecmgqdpetafctlanrdassadkcSIQHCLYQ 630
           ***************************************************************************************** PP
   S: 194  ftkeeelegenkyycekckkkqe.................aekklklkklPevLilhLkRfsfseeqksrekinkkvefpleLdlspfc 265
           ft++e+l+++nk+ ce c+++q                  a+k++ ++  P+vL+lhLkRf  ++++ + +kink+++fp+ Ldl+pfc
   Q: 631  FTQNEKLQDANKLLCEVCTRRQCsapkanlkgekkhvytnAKKQVLISLAPPVLTLHLKRF--QQAGFNLHKINKHIKFPEILDLAPFC 717
           *******************888899999999999999999*********************..999*********************** PP
   S: 266  tspeekqke...kkkYeLiavvvHsGslssGHYvayvkn........................ekgkWllfdDskVtevkeeevleetg 327
           t ++++ +e   ++ Y+L++vv HsG+l sGHY+ay k                         ++g+W++++Ds+V++v+ ++vl+   
   Q: 718  TAKCKDMAEestRVMYSLYGVVEHSGTLMSGHYTAYTKArmaksrlsdlvlrgdvpqdfemesTEGQWFHISDSHVQAVPTAKVLN--- 803
           **998887777799*************************9999999999999999999999888999*******************... PP
   S: 328  staYvlfYkr 337
           s+aY+lfY+r
   Q: 804  SQAYLLFYER 813
           ********98 PP

Organism

Myotis lucifugus

Functional Description
(View)

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.

Protein Sequence
(Fasta)

MGKKRIKGKI VPVDDSSKSL EPVCRHIRKG LEQSILKKAL VNVEWNICQD CETDNKVKDK 60
SEEETEENPS IWLCLKCGHQ GCGRNSQEQH ALKHSQVPRS EPHCLVLSLD NWSVWCYLCD 120

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Nucleotide Sequence
(Fasta)

ATGGGAAAGA AACGGATAAA GGGAAAAATT GTTCCAGTTG ATGATTCTTC TAAATCTTTA 60
GAACCTGTGT GCAGACACAT TAGAAAAGGA CTGGAACAAA GTATTCTGAA AAAGGCTTTG 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0010--Activator
KW-0131--Cell cycle
KW-0132--Cell division
KW-0156--Chromatin regulator
KW-0175--Coiled coil
KW-0181--Complete proteome
KW-0378--Hydrolase
KW-0479--Metal-binding
KW-0498--Mitosis
KW-0539--Nucleus
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0804--Transcription
KW-0805--Transcription regulation
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR001394--Peptidase_C19_UCH
IPR030849--UBP16
IPR018200--USP_CS
IPR028889--USP_dom
IPR013083--Znf_RING/FYVE/PHD
IPR001607--Znf_UBP

PROSITE

PS00972--USP_1
PS00973--USP_2
PS50235--USP_3
PS50271--ZF_UBP

Pfam

PF00443--UCH
PF02148--zf-UBP

SMART

SM00290--ZnF_UBP

Gene Ontology

GO:0005634--C:nucleus
GO:0004197--F:cysteine-type endopeptidase activity
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:0003713--F:transcription coactivator activity
GO:0008270--F:zinc ion binding
GO:0051301--P:cell division
GO:0016578--P:histone deubiquitination
GO:0007067--P:mitotic nuclear division
GO:0045893--P:positive regulation of transcription, DNA-templated
GO:0051289--P:protein homotetramerization
GO:0051726--P:regulation of cell cycle
GO:0006351--P:transcription, DNA-templated
GO:0006511--P:ubiquitin-dependent protein catabolic process

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Loc-051814	Lepisosteus oculatus	48.00	0.00e+00	662.00
IUUC-Mvi-060391	Microbotryum violaceum	33.67	1.00e-19	92.40
IUUC-Opu-083452	Oryza punctata	29.19	5.00e-29	123.00
IUUC-Oru-084546	Oryza rufipogon	29.45	3.00e-29	124.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence