iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Single Nucleotide Polymorphisms (SNP)
dbSNP
mRNA Expression
GEO	ArrayExpress
DNA & RNA Element
TRANSFAC
Protein-protein Interaction
iRefIndex	HINT	Mentha
Post-translational Modifications (PTMs)
dbPPT	dbPTM	UniProt
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
E3 activity/HECT	HECT	12969426

Classification

Family	E-value	Score	Start	End
E3 activity/HECT	3.30e-121	405.7	3318	3657
UBD/Alpha-Helix/UBA	7.20e-07	30.0	1274	1311

Active Site

Position(s)	Description	Evidence
3625	Glycyl thioester intermediate	{ECO:0000255\|PROSITE-ProRule:PRU00104}

Domain Profile

E3 activity/HECT

   S: 2    ksdlkkrlevefvgEegldlgGvtreflallskelqnpdlglflysendketlqpnpdsglknpehlklFeflGrllgkalqdgrlldlplsksfy 97
           ++dlk rl+v+f+gEeg+d+gG+tre+++lls+ ++++ + lf ++ ++++t+qpnp+s ++ +ehl++F+f+Gr+++kal+dg+lld+ +++sfy
   Q: 3318 PQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLF-TTVGNDATFQPNPNSVYQ-TEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFY 3411
           689************************************9999.556678889******986.5******************************** PP
   S: 98   klllgkklsledleevdpelykslkeikenaesdieelgldltfsvseei.......lgetkeeeLkpgGenipVtneNkeeYvklvveyrlkegi 186
           k++lg+k++++d+e+vdp++yk+lk+++en   d++++ ldltfs++ ++         e++++eLkpgG+ni+Vt+e k+eYv+lv++++l++ i
   Q: 3412 KHILGVKVTYHDIEAVDPDYYKNLKWLLEN---DVSDI-LDLTFSMDADEekhilyeKTEVTDYELKPGGRNIRVTEETKHEYVDLVADHILTSAI 3503
           ****************************99...88887.88888866666668895555566778******************************* PP
   S: 187  keqveafreGfneviplellslFteeelellicGeeeidvedlkketkykggytedspaiqwFwevleefsseerkafLqFvTgssrlPlgGfael 282
           + q++af eG+ne+ip el+s+F+ +elelli+G +eid++dlk++t+y++ yt  sp+i+wFwev+++fs+e+ ++fLqFvTg+s++Pl+Gf++l
   Q: 3504 RPQINAFLEGLNELIPRELVSIFNDKELELLISGLPEIDFDDLKANTEYTS-YTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKAL 3598
           *************************************************99.******************************************** PP
   S: 283  k....prlfvirk.dasddrlPssnTCfnyLkLPeYsskeilkekLlyAieet.kgFel 335
           +    p++ +i+k ++s++rlPs++TCfn+L+LPeY+ske+++e+Ll+Ai+e+ +gF++
   Q: 3599 QgisgPQRLQIHKaYGSPERLPSAHTCFNQLDLPEYQSKEQVQERLLLAIHEAnEGFGF 3657
           *************999*************************************9***86 PP

UBD/Alpha-Helix/UBA

   S: 4    edkleqlveMGFdreealqaLraa.nnnleaAveyLld 40
           e ++  +veMGF+r++a  aLr+   n++e+A+++L++
   Q: 1274 EAIVGMIVEMGFSRSRAEDALRRVgTNSVEMAMDWLFT 1311
           55666789*****************************8 PP

Organism

Arabidopsis thaliana

Functional Description
(View)

Probable E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins.

Protein Sequence
(Fasta)

MKLRRRRASE VPTKISLFIN SVTSVPLELI QEPLASFRWE FDKGDFHHWV DLFYHFDTFF 60
EKHVKVRKDL RIEEEFDESD PPFPKDAVLQ VLRVIRLVLE NCTNKQFYTS YEQHLSLLLA 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

TCTCTCTCTC ACACCGTCTT TCTCTCTCCC TATCAAATCC CTTCTCTCTA ATATGCGATC 60
TTCAAAACCC TAGTTTCTTC GGATCGCTTC CAAACTTGCG ATTATCTCCA AGATAGTCAA 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0597--Phosphoprotein
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway

Interpro

IPR011989--ARM-like
IPR016024--ARM-type_fold
IPR025527--DUF4414
IPR010309--E3_Ub_ligase_DUF908
IPR010314--E3_Ub_ligase_DUF913
IPR000569--HECT_dom
IPR015940--UBA
IPR009060--UBA-like
IPR003903--UIM_dom

PROSITE

PS50237--HECT
PS50030--UBA
PS50330--UIM

Pfam

PF14377--DUF4414
PF06012--DUF908
PF06025--DUF913
PF00632--HECT
PF00627--UBA

SMART

SM00119--HECTc
SM00165--UBA

Gene Ontology

GO:0005829--C:cytosol
GO:0005739--C:mitochondrion
GO:0005634--C:nucleus
GO:0004842--F:ubiquitin-protein transferase activity

KEGG

ath:AT1G70320

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Ata-000627	Aegilops tauschii	35.62	0.00e+00	1048.00
IUUC-Apl-003400	Anas platyrhynchos	42.53	2.00e-94	338.00
IUUC-Ago-008005	Ashbya gossypii	38.66	1.00e-123	438.00
IUUC-Ani-009412	Aspergillus nidulans	43.24	2.00e-139	491.00
IUUC-Aor-010186	Aspergillus oryzae	39.47	2.00e-129	456.00
IUUC-Bol-015228	Brassica oleracea	35.55	0.00e+00	939.00
IUUC-Cpo-021766	Cavia porcellus	42.99	1.00e-95	343.00
IUUC-Cgl-026647	Colletotrichum gloeosporioides	39.11	1.00e-135	478.00
IUUC-Cne-027095	Cryptococcus neoformans	40.35	5.00e-110	393.00
IUUC-Cme-027267	Cyanidioschyzon merolae	50.91	2.00e-125	444.00
IUUC-Ete-032966	Echinops telfairi	42.76	1.00e-95	344.00
IUUC-Fal-036687	Ficedula albicollis	41.86	6.00e-95	341.00
IUUC-Fox-037756	Fusarium oxysporum	31.21	2.00e-147	518.00
IUUC-Fso-038464	Fusarium solani	48.78	3.00e-135	477.00
IUUC-Ggr-039946	Gaeumannomyces graminis	39.84	1.00e-133	472.00
IUUC-Ggo-044465	Gorilla gorilla	42.66	5.00e-94	338.00
IUUC-Hvu-047697	Hordeum vulgare	56.25	0.00e+00	1069.00
IUUC-Kpa-049228	Komagataella pastoris	40.63	2.00e-127	451.00
IUUC-Meu-056436	Macropus eugenii	40.40	3.00e-77	282.00
IUUC-Mor-057201	Magnaporthe oryzae	36.60	2.00e-134	474.00
IUUC-Mpo-057597	Magnaporthe poae	38.35	3.00e-133	471.00
IUUC-Mga-059935	Meleagris gallopavo	42.53	1.00e-94	340.00
IUUC-Ncr-068813	Neurospora crassa	39.91	6.00e-143	503.00
IUUC-Ome-081289	Oryza meridionalis	65.54	0.00e+00	1058.00
IUUC-Osa-086200	Oryza sativa	63.52	0.00e+00	1221.00
IUUC-Ola-086777	Oryzias latipes	42.53	1.00e-96	347.00
IUUC-Ptr-090529	Pan troglodytes	42.76	2.00e-95	343.00
IUUC-Psi-093969	Pelodiscus sinensis	42.53	4.00e-95	342.00
IUUC-Rno-105153	Rattus norvegicus	42.76	9.00e-96	344.00
IUUC-Sce-106449	Saccharomyces cerevisiae	31.71	1.00e-126	448.00
IUUC-Sja-107814	Schizosaccharomyces japonicus	32.37	1.00e-130	462.00
IUUC-Spo-108024	Schizosaccharomyces pombe	34.01	2.00e-140	494.00
IUUC-Stu-112501	Solanum tuberosum	67.38	5.00e-132	463.00
IUUC-Tgu-117225	Taeniopygia guttata	41.63	1.00e-94	340.00
IUUC-Tvi-122370	Trichoderma virens	39.04	8.00e-139	489.00
IUUC-Tae-125214	Triticum aestivum	54.36	0.00e+00	1055.00
IUUC-Tme-127172	Tuber melanosporum	39.87	7.00e-132	466.00
IUUC-Vda-129886	Verticillium dahliae	47.75	1.00e-135	478.00
IUUC-Yli-134657	Yarrowia lipolytica	40.58	5.00e-134	473.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence