iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Tag

Content

UUCD2 ID

IUUC-Aca-005036

UUCD1 version

UUC-AnC-00472

Ensembl Protein ID

ENSACAP00000001665.2

UniProt Accession

G1K9T9; G1K9T9_ANOCA

Protein Name

E3 ubiquitin-protein ligase RNF8; RING finger protein 8; RING-type E3 ubiquitin transferase RNF8

Gene Name

RNF8

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
ENSACAG00000001771.2	ENSACAT00000001704.2	ENSACAP00000001665.2

Status

Unreviewed

Classification

Family	E-Value	Score	Start	End
E3 activity/RING/RING	7.40e-07	29.6	408	446

Active Site

Position(s)	Description	Evidence
N/A	N/A	N/A

Domain Profile

E3 activity/RING/RING

   S: 1    CaiCledfkdgplvlpCgHvfhadClqkwpglknssskefrCPlCr 46
           C iC e+f +  ++l+C H f+  C+ +w       + +  CP+Cr
   Q: 408  CTICSEHFIE-AVTLNCAHSFCSFCIDQW------MKLRLECPICR 446
           ********99.899***************......**********8 PP

Organism

Anolis carolinensis

Functional Description
(View)

E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains, leading to degradation of substrate proteins. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation.

Protein Sequence
(Fasta)

AGGGGAIGAL SEEPARIWCL KRAGLSEEWL LLEGGSEITI GRGAGVTYQL ISKSCPLMIS 60
RNHCVFKQNT EGQWTVTDNK SLNGVWLNCE RIDPLKPYCL QEGDFIQLGV PLKHTETAEY 120

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Nucleotide Sequence
(Fasta)

GCGGGTGGTG GCGGCGCTAT TGGCGCCCTC TCAGAGGAGC CGGCTCGGAT TTGGTGCCTG 60
AAGCGCGCGG GCCTGAGCGA GGAATGGCTG CTGCTGGAAG GCGGGAGCGA GGTGAGCGCA 120

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Sequence Source

Ensembl

Keyword

KW-0156--Chromatin regulator
KW-0181--Complete proteome
KW-0227--DNA damage
KW-0234--DNA repair
KW-0479--Metal-binding
KW-0539--Nucleus
KW-1185--Reference proteome
KW-0808--Transferase
KW-0833--Ubl conjugation pathway
KW-0862--Zinc
KW-0863--Zinc-finger

Interpro

IPR000253--FHA_dom
IPR017335--RNF8
IPR008984--SMAD_FHA_domain
IPR001841--Znf_RING
IPR013083--Znf_RING/FYVE/PHD
IPR017907--Znf_RING_CS

PROSITE

PS50006--FHA_DOMAIN
PS00518--ZF_RING_1
PS50089--ZF_RING_2

Pfam

PF00498--FHA

SMART

SM00240--FHA
SM00184--RING

Gene Ontology

GO:0005829--C:cytosol
GO:0005634--C:nucleus
GO:0035861--C:site of double-strand break
GO:0000151--C:ubiquitin ligase complex
GO:0003682--F:chromatin binding
GO:0004842--F:ubiquitin-protein transferase activity
GO:0008270--F:zinc ion binding
GO:0006302--P:double-strand break repair
GO:0070535--P:histone H2A K63-linked ubiquitination
GO:0034244--P:negative regulation of transcription elongation from RNA polymerase II promoter
GO:0045739--P:positive regulation of DNA repair
GO:0051865--P:protein autoubiquitination
GO:0070936--P:protein K48-linked ubiquitination
GO:0010212--P:response to ionizing radiation
GO:0006511--P:ubiquitin-dependent protein catabolic process

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-002124	Ailuropoda melanoleuca	56.96	4.00e-107	381.00
IUUC-Apl-003664	Anas platyrhynchos	80.43	1.00e-39	154.00
IUUC-Ame-011537	Astyanax mexicanus	41.91	4.00e-83	301.00
IUUC-Bta-013297	Bos taurus	57.72	1.00e-112	399.00
IUUC-Cja-019201	Callithrix jacchus	57.20	3.00e-114	405.00
IUUC-Cfa-021041	Canis familiaris	58.40	6.00e-119	420.00
IUUC-Csa-024133	Chlorocebus sabaeus	57.26	2.00e-120	425.00
IUUC-Cho-024624	Choloepus hoffmanni	50.12	6.00e-65	241.00
IUUC-Cgl-026486	Colletotrichum gloeosporioides	38.10	6.00e-07	49.30
IUUC-Dre-027918	Danio rerio	42.94	3.00e-80	292.00
IUUC-Dno-029774	Dasypus novemcinctus	59.36	4.00e-118	417.00
IUUC-Dor-030190	Dipodomys ordii	52.24	1.00e-91	330.00
IUUC-Ete-032301	Echinops telfairi	53.30	2.00e-81	296.00
IUUC-Eca-033607	Equus caballus	57.21	2.00e-98	352.00
IUUC-Eeu-034888	Erinaceus europaeus	61.00	4.00e-67	248.00
IUUC-Fca-036075	Felis catus	57.47	1.00e-116	412.00
IUUC-Fal-037569	Ficedula albicollis	64.49	4.00e-116	411.00
IUUC-Fox-037681	Fusarium oxysporum	36.51	9.00e-06	45.10
IUUC-Gmo-038588	Gadus morhua	39.08	5.00e-66	244.00
IUUC-Ggr-039871	Gaeumannomyces graminis	38.10	8.00e-07	48.90
IUUC-Gga-040777	Gallus gallus	61.92	5.00e-120	424.00
IUUC-Gac-042463	Gasterosteus aculeatus	38.99	9.00e-62	231.00
IUUC-Ggo-045269	Gorilla gorilla	57.05	2.00e-119	422.00
IUUC-Hsa-046249	Homo sapiens	56.42	5.00e-116	410.00
IUUC-Itr-048314	Ictidomys tridecemlineatus	58.02	1.00e-120	426.00
IUUC-Kpa-049300	Komagataella pastoris	44.68	1.00e-07	50.10
IUUC-Lch-049805	Latimeria chalumnae	50.10	4.00e-98	351.00
IUUC-Loc-052377	Lepisosteus oculatus	47.05	5.00e-88	318.00
IUUC-Mcc-055344	Macaca mulatta	56.84	7.00e-120	423.00
IUUC-Meu-055950	Macropus eugenii	54.68	4.00e-96	344.00
IUUC-Mor-057012	Magnaporthe oryzae	39.68	1.00e-07	51.60
IUUC-Mga-060049	Meleagris gallopavo	63.33	8.00e-120	423.00
IUUC-Mmr-061431	Microcebus murinus	58.99	2.00e-117	415.00
IUUC-Mdo-062016	Monodelphis domestica	61.35	8.00e-102	365.00
IUUC-Mmu-063235	Mus musculus	56.24	3.00e-112	398.00
IUUC-Mpu-066449	Mustela putorius furo	57.92	7.00e-123	433.00
IUUC-Mlu-067588	Myotis lucifugus	57.63	8.00e-110	390.00
IUUC-Ncr-068692	Neurospora crassa	38.10	1.00e-06	48.90
IUUC-Nle-069192	Nomascus leucogenys	57.26	2.00e-119	422.00
IUUC-Opr-070300	Ochotona princeps	46.71	1.00e-89	323.00
IUUC-Ont-071998	Oreochromis niloticus	42.50	7.00e-65	241.00
IUUC-Oan-073610	Ornithorhynchus anatinus	59.65	2.00e-47	181.00
IUUC-Ocu-074603	Oryctolagus cuniculus	56.63	4.00e-111	394.00
IUUC-Ola-086681	Oryzias latipes	39.47	9.00e-74	270.00
IUUC-Olu-087723	Ostreococcus lucimarinus	37.78	5.00e-07	49.70
IUUC-Oga-088183	Otolemur garnettii	58.02	6.00e-119	421.00
IUUC-Oar-090255	Ovis aries	56.98	1.00e-100	360.00
IUUC-Ptr-091029	Pan troglodytes	56.18	2.00e-111	395.00
IUUC-Pan-092241	Papio anubis	57.05	9.00e-120	423.00
IUUC-Psi-093506	Pelodiscus sinensis	68.03	8.00e-134	470.00
IUUC-Pma-094579	Petromyzon marinus	60.00	6.00e-24	105.00
IUUC-Pfo-097014	Poecilia formosa	41.30	6.00e-68	251.00
IUUC-Pab-098530	Pongo abelii	57.05	3.00e-114	405.00
IUUC-Pca-100939	Procavia capensis	45.24	5.00e-80	291.00
IUUC-Pva-103148	Pteropus vampyrus	53.80	3.00e-103	368.00
IUUC-Rno-105338	Rattus norvegicus	56.63	4.00e-118	417.00
IUUC-Sce-106402	Saccharomyces cerevisiae	33.70	2.00e-07	50.40
IUUC-Sha-107187	Sarcophilus harrisii	58.62	4.00e-78	285.00
IUUC-Sar-113356	Sorex araneus	47.80	2.00e-75	276.00
IUUC-Ssc-116290	Sus scrofa	58.09	4.00e-127	447.00
IUUC-Tru-117910	Takifugu rubripes	41.35	4.00e-65	241.00
IUUC-Tsy-119329	Tarsius syrichta	54.07	1.00e-92	333.00
IUUC-Tni-120521	Tetraodon nigroviridis	38.17	5.00e-62	231.00
IUUC-Tre-121954	Trichoderma reesei	36.51	9.00e-06	45.10
IUUC-Tvi-122295	Trichoderma virens	33.73	2.00e-06	47.00
IUUC-Ttr-128997	Tursiops truncatus	52.11	9.00e-100	357.00
IUUC-Vda-130024	Verticillium dahliae	32.67	3.00e-08	53.10
IUUC-Vpa-130154	Vicugna pacos	56.48	3.00e-118	418.00
IUUC-Xtr-132805	Xenopus tropicalis	49.16	9.00e-97	347.00
IUUC-Xma-133620	Xiphophorus maculatus	42.20	3.00e-67	249.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence