iUUCD 2.0 - integrated annotations for ubiquitin and ubiquitin-like conjugation database

Protein
Gene
Annotation
Details
Classification
Domain Profile
Function
Protein Sequence
Nucleotide Sequence
Keyword
GO
Orthology
TOP

Basic Information

Integrated Annotations

Tag

Content

iUUCD ID

IUUC-Sce-106166

UUCD1 version

UUC-SaC-00234

Ensembl Protein ID

YDR069C

UniProt Accession

P32571; D6VS55; UBP4_YEAST

Genbank Protein ID

AAC48915.1; AAA35105.1; CAA89098.1; CAA86791.1; CAA98887.1; CAA58985.1; DAA11915.1

Protein Name

Ubiquitin carboxyl-terminal hydrolase 4; Deubiquitinating enzyme 4; Ubiquitin thioesterase 4; Ubiquitin-specific-processing protease 4; Vacuole biogenesis protein SSV7

Genbank Nucleotide ID

U02518; L08070; Z49209; Z46796; Z74365; X84162; BK006938

Gene Name

DOA4; DOS1; MUT4; NPI2; SSV7; UBP4; YDR069C; D4270; YD8554.02C; YD9609.23C

Ensembl Information

Ensembl Gene ID	Ensembl Transcript ID	Ensembl Protein ID
YDR069C	YDR069C	YDR069C

Annotation

mRNA Expression
GEO	FFGED
Protein-protein Interaction
IID	iRefIndex	HINT	Mentha
Post-translational Modifications (PTMs)
CPLM	dbPAF	dbPTM	UniProt
BioGRID
Protein Expression/Proteomics
GPMDB

Status

Reviewed

Details

Family	Domain	References (PMIDs)
DUB/USP	USP	17145966

Classification

Family	E-value	Score	Start	End
DUB/USP	2.40e-92	308.1	562	922

Active Site

Position(s)	Description	Evidence
571	Nucleophile
880	Proton acceptor	ECO:0000255\|PROSITE-ProRule:PRU10092, ECO:0000255\|PROSITE-ProRule:PRU10093}

Domain Profile

DUB/USP

   S: 1    tGlkNlgNtCYmNSvLQcLsnipelrellle.kkkeklneenpkepkgellkslqklfeelw.....kskkkavsprellkalkkeaeefsgyeqqDaqEfl 96
           +Gl+NlgN+CYmN+++Qc++ ++el++ +l+ ++ +++n +++ ++kg l+k +++l++ ++      skk ++sp +++ a++++++ f++ +qqD+qEf+
   Q: 562  VGLENLGNSCYMNCIIQCILGTHELTQIFLDdSYAKHININSKLGSKGILAKYFARLVHMMYkeqvdGSKKISISPIKFKLACGSVNSLFKTASQQDCQEFC 663
           6******************************99****************************98777777777****************************** PP
   S: 97   aflldqleeelkkeknkpkeeeeeedkselsde.........aeekkkkknkslisdlFqgqlksqikclecdnvssreepfldlslpvpgkeetkkeslqd 189
           +flld+l+e+l++  ++p  +e +++ +++ ++         ++e+  +   s+i+dlFqgq+ s++kc+ c ++s++++pf+ ls+p+p+k+++++ +++d
   Q: 664  QFLLDGLHEDLNQCGSNPPLKELSQEAEARREKlslriassiEWERFLTTDFSVIVDLFQGQYASRLKCKVCSHTSTTYQPFTVLSIPIPKKNSRNNITIED 765
           ***********9966666666666666666666689999999************************************************************ PP
   S: 190  cLesftkeeelegenkyycekckkkqeaekklklkklPevLilhLkRfsfseeqksrekinkkvefpleLdlspfcts...............peekqkekk 276
           c+++ftk e+le ++++ c++c+k+q ++k+l++++lP+ Li+hLkRf      +  +k+n+ v +p  Ldl+pf ++               p ++q  + 
   Q: 766  CFREFTKCENLEVDEQWLCPHCEKRQPSTKQLTITRLPRNLIVHLKRF-----DNLLNKNNDFVIYPFLLDLTPFWANdfdgvfppgvnddelPIRGQIPPF 862
           ************************************************.....57899****************99996777777666555554444444** PP
   S: 277  kYeLiavvvHsGslssGHYvayvknekgk.WllfdDskVtevke.eevleetgstaYvlfYkr 337
           kYeL++v +H G+l +GHY+ayvk+  +k Wl+fdD+k ++vk  ++  +   s+aYvlfY+r
   Q: 863  KYELYGVACHFGTLYGGHYTAYVKKGLKKgWLYFDDTKYKPVKNkADAIN---SNAYVLFYHR 922
           *************************54445************85267777...9*******97 PP

Organism

Saccharomyces cerevisiae

Functional Description
(View)

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Involved in the ammonium-induced down-regulation of the GAP1 permease and the UME3 destruction in response to oxidative stress. Has a role in the RAD9 checkpoint response to TOP1 poisons. Required for promoting coordination of DNA replication and avoids DNA overreplication.

Protein Sequence
(Fasta)

MEQNIISTIR DECIRHRSKY LTIAQLTAIA EAKINEFIIT GKAKDQDLSS LLDKCIDILS 60
IYKKNSKDIK NIISCKNKGA MISSNSVMII QLNYVYYKVI HIIVTTNIPH LSEFAKIKLH 120

It may take some time, please wait.

Nucleotide Sequence
(Fasta)

ATGGAGCAGA ATATTATTAG TACCATAAGG GATGAGTGTA TTCGTCACCG GTCGAAGTAC 60
CTTACGATAG CACAACTAAC CGCTATTGCA GAGGCTAAAA TTAACGAATT CATCATAACT 120

It may take some time, please wait.

Sequence Source

Ensembl

Keyword

KW-0181--Complete proteome
KW-0963--Cytoplasm
KW-0967--Endosome
KW-0378--Hydrolase
KW-0472--Membrane
KW-0597--Phosphoprotein
KW-0645--Protease
KW-1185--Reference proteome
KW-0788--Thiol protease
KW-0833--Ubl conjugation pathway

Interpro

IPR001394--Peptidase_C19_UCH
IPR001763--Rhodanese-like_dom
IPR018200--USP_CS
IPR028889--USP_dom

PROSITE

PS50206--RHODANESE_3
PS00972--USP_1
PS00973--USP_2
PS50235--USP_3

Pfam

PF00581--Rhodanese
PF00443--UCH

SMART

SM00450--RHOD

Gene Ontology

GO:0005768--C:endosome
GO:0031902--C:late endosome membrane
GO:0000502--C:proteasome complex
GO:0004843--F:thiol-dependent ubiquitin-specific protease activity
GO:1904669--P:ATP export
GO:0006897--P:endocytosis
GO:0010995--P:free ubiquitin chain depolymerization
GO:0070676--P:intralumenal vesicle formation
GO:0016579--P:protein deubiquitination
GO:0006275--P:regulation of DNA replication
GO:0010992--P:ubiquitin homeostasis
GO:0006511--P:ubiquitin-dependent protein catabolic process
GO:0043162--P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

KEGG

sce:YDR069C

Orthology

iUUCD ID	Species	Identity	E-value	Score
IUUC-Aml-001661	Ailuropoda melanoleuca	35.26	7.00e-54	204.00
IUUC-Apl-003226	Anas platyrhynchos	35.26	4.00e-54	204.00
IUUC-Aca-005132	Anolis carolinensis	36.46	2.00e-57	215.00
IUUC-Ago-007959	Ashbya gossypii	45.67	0.00e+00	719.00
IUUC-Afl-008604	Aspergillus flavus	36.27	1.00e-66	246.00
IUUC-Ang-009844	Aspergillus niger	36.08	1.00e-65	242.00
IUUC-Aor-010098	Aspergillus oryzae	36.27	6.00e-67	246.00
IUUC-Ate-010585	Aspergillus terreus	37.33	1.00e-66	246.00
IUUC-Ame-011102	Astyanax mexicanus	33.51	8.00e-46	175.00
IUUC-Bta-013008	Bos taurus	35.54	1.00e-54	206.00
IUUC-Cja-019173	Callithrix jacchus	35.54	1.00e-53	203.00
IUUC-Cfa-021243	Canis familiaris	35.26	8.00e-54	203.00
IUUC-Cpo-021522	Cavia porcellus	35.26	2.00e-53	202.00
IUUC-Cre-022582	Chlamydomonas reinhardtii	34.13	1.00e-55	207.00
IUUC-Csa-023616	Chlorocebus sabaeus	35.81	8.00e-54	203.00
IUUC-Cin-025797	Ciona intestinalis	39.08	5.00e-64	235.00
IUUC-Csv-026132	Ciona savignyi	38.32	3.00e-60	223.00
IUUC-Cne-026821	Cryptococcus neoformans	40.29	2.00e-80	292.00
IUUC-Dre-027476	Danio rerio	34.60	2.00e-45	174.00
IUUC-Dno-029039	Dasypus novemcinctus	35.26	6.00e-54	204.00
IUUC-Dor-031053	Dipodomys ordii	31.99	5.00e-48	183.00
IUUC-Dse-031463	Dothistroma septosporum	32.20	7.00e-54	204.00
IUUC-Ete-033040	Echinops telfairi	36.00	3.00e-41	161.00
IUUC-Eca-033449	Equus caballus	35.54	9.00e-55	206.00
IUUC-Fca-035794	Felis catus	35.26	4.00e-53	200.00
IUUC-Fal-037322	Ficedula albicollis	35.54	6.00e-54	204.00
IUUC-Gga-041188	Gallus gallus	35.54	2.00e-53	202.00
IUUC-Gac-041850	Gasterosteus aculeatus	35.54	1.00e-53	202.00
IUUC-Ggo-045565	Gorilla gorilla	35.54	8.00e-54	203.00
IUUC-Hsa-046438	Homo sapiens	35.54	1.00e-53	203.00
IUUC-Itr-048883	Ictidomys tridecemlineatus	35.26	6.00e-54	204.00
IUUC-Kpa-049321	Komagataella pastoris	48.13	1.00e-98	352.00
IUUC-Lch-050485	Latimeria chalumnae	35.26	2.00e-54	205.00
IUUC-Loc-052769	Lepisosteus oculatus	37.24	5.00e-54	204.00
IUUC-Laf-053432	Loxodonta africana	34.71	3.00e-53	202.00
IUUC-Mcc-055823	Macaca mulatta	35.81	3.00e-54	205.00
IUUC-Meu-056279	Macropus eugenii	31.59	3.00e-47	181.00
IUUC-Mla-059089	Melampsora laricipopulina	41.64	2.00e-64	239.00
IUUC-Mga-059739	Meleagris gallopavo	35.54	4.00e-53	201.00
IUUC-Mvi-060559	Microbotryum violaceum	37.36	8.00e-68	250.00
IUUC-Mmr-061158	Microcebus murinus	34.99	2.00e-53	202.00
IUUC-Mdo-061801	Monodelphis domestica	34.99	3.00e-53	201.00
IUUC-Mmu-063665	Mus musculus	34.71	3.00e-54	205.00
IUUC-Mpu-066738	Mustela putorius furo	35.26	8.00e-54	203.00
IUUC-Mlu-067307	Myotis lucifugus	34.44	1.00e-53	202.00
IUUC-Nfi-068410	Neosartorya fischeri	36.12	2.00e-63	235.00
IUUC-Nle-069314	Nomascus leucogenys	35.26	9.00e-54	203.00
IUUC-Opr-070907	Ochotona princeps	33.60	6.00e-46	176.00
IUUC-Oan-073537	Ornithorhynchus anatinus	34.15	2.00e-51	196.00
IUUC-Ocu-074193	Oryctolagus cuniculus	35.26	7.00e-54	203.00
IUUC-Oga-088271	Otolemur garnettii	35.26	1.00e-53	203.00
IUUC-Oar-090431	Ovis aries	35.54	2.00e-54	206.00
IUUC-Ptr-091339	Pan troglodytes	35.54	8.00e-54	203.00
IUUC-Pan-092266	Papio anubis	34.44	8.00e-49	185.00
IUUC-Pma-094626	Petromyzon marinus	35.16	4.00e-57	213.00
IUUC-Pab-097645	Pongo abelii	35.54	1.00e-53	203.00
IUUC-Pca-100205	Procavia capensis	33.24	1.00e-43	169.00
IUUC-Pva-103222	Pteropus vampyrus	33.88	2.00e-50	192.00
IUUC-Pgr-103408	Puccinia graminis	35.29	5.00e-49	187.00
IUUC-Ptt-103699	Puccinia triticina	44.60	9.00e-60	223.00
IUUC-Pte-104348	Pyrenophora teres	34.58	1.00e-54	206.00
IUUC-Rno-105510	Rattus norvegicus	34.99	2.00e-53	202.00
IUUC-Sha-106879	Sarcophilus harrisii	34.70	7.00e-52	197.00
IUUC-Sja-107836	Schizosaccharomyces japonicus	36.71	2.00e-59	221.00
IUUC-Spo-108065	Schizosaccharomyces pombe	32.02	4.00e-52	197.00
IUUC-Smo-109021	Selaginella moellendorffii	28.84	8.00e-36	142.00
IUUC-Sar-113019	Sorex araneus	35.25	2.00e-54	205.00
IUUC-Sre-115031	Sporisorium reilianum	38.70	1.00e-72	266.00
IUUC-Ssc-116334	Sus scrofa	35.26	4.00e-54	204.00
IUUC-Tgu-117062	Taeniopygia guttata	35.54	1.00e-53	203.00
IUUC-Tru-117908	Takifugu rubripes	35.54	1.00e-54	206.00
IUUC-Tsy-118879	Tarsius syrichta	34.24	7.00e-45	172.00
IUUC-Tni-120206	Tetraodon nigroviridis	35.87	7.00e-52	197.00
IUUC-Tme-127144	Tuber melanosporum	45.55	2.00e-87	315.00
IUUC-Ttr-128580	Tursiops truncatus	35.81	9.00e-55	206.00
IUUC-Uma-129340	Ustilago maydis	36.39	3.00e-68	251.00
IUUC-Vpa-130541	Vicugna pacos	34.44	1.00e-51	196.00
IUUC-Xtr-132161	Xenopus tropicalis	34.79	2.00e-55	209.00
IUUC-Yli-134561	Yarrowia lipolytica	42.80	2.00e-88	318.00

Created Date

25-Jun-2017

Functional Description

Protein Fasta Sequence

Nucleotide Fasta Sequence