PTM - CPLM Gene Name UniProt ID Type Position Code PSEN1; AD3; PS1; PSNL1 P49768 Ubiquitination 314 K PSEN1; AD3; PS1; PSNL1 P49768 Ubiquitination 109 K PSEN1; AD3; PS1; PSNL1 P49768 Ubiquitination 76 K PSEN1; AD3; PS1; PSNL1 P49768 Ubiquitination 311 K

PTM - dbPAF Gene Name UniProt ID Position Type Species PSEN1; AD3; PS1; PSNL1 P49768 102 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 104 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 107 T Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 154 Y Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 240 Y Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 256 Y Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 310 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 319 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 320 T Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 324 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 337 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 346 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 353 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 354 T Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 357 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 365 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 366 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 367 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 43 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 51 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 59 S Homo sapiens(Human) PSEN1; AD3; PS1; PSNL1 P49768 99 T Homo sapiens(Human)

PTM - PhosSNP Gene Name dbSNP ID Gene Mutation AA Mutation Mutation Position Code Peptide Score Modification Type PSEN1; AD3; PS1; PSNL1 rs63751164 ACA->ATA T->I 354 T HLGPHRSTPESRAAV 354 Phosphorylation: CDK5; CDK_group TypeI- PSEN1; AD3; PS1; PSNL1 rs63751164 ACA->ATA T->I 350 T HLGPHRSTPESRAAV 354 Phosphorylation: CDK5; CDK_group TypeI- PSEN1; AD3; PS1; PSNL1 rs63750941 TCC->TAC S->Y 367 S AVQELSSSILAGEDP 367 Phosphorylation TypeIII- PSEN1; AD3; PS1; PSNL1 rs63751164 ACA->ATA T->I 353 S SHLGPHRSTPESRAA 353 Phosphorylation: GSK-3beta TypeIII- PSEN1; AD3; PS1; PSNL1 rs63751164 ACA->ATA T->I 357 S PHRSTPESRAAVQEL 357 Phosphorylation: GSK-3beta TypeIII- PSEN1; AD3; PS1; PSNL1 rs63751174 CGA->CAA R->Q 353 S SHLGPHRSTPESRAA 353 Phosphorylation: GSK-3beta TypeIII- PSEN1; AD3; PS1; PSNL1 rs63751174 CGA->CAA R->Q 357 S PHRSTPESRAAVQEL 357 Phosphorylation: GSK-3beta TypeIII- PSEN1; AD3; PS1; PSNL1 rs63750941 TCC->TAC S->Y 363 S AVQELSSSILAGEDP 367 Phosphorylation TypeIII- PSEN1; AD3; PS1; PSNL1 rs63751164 ACA->ATA T->I 349 S SHLGPHRSTPESRAA 353 Phosphorylation: GSK-3beta TypeIII- PSEN1; AD3; PS1; PSNL1 rs63751164 ACA->ATA T->I 353 S PHRSTPESRAAVQEL 357 Phosphorylation: GSK-3beta TypeIII- PSEN1; AD3; PS1; PSNL1 rs63751174 CGA->CAA R->Q 349 S SHLGPHRSTPESRAA 353 Phosphorylation: GSK-3beta TypeIII- PSEN1; AD3; PS1; PSNL1 rs63751174 CGA->CAA R->Q 353 S PHRSTPESRAAVQEL 357 Phosphorylation: GSK-3beta TypeIII-

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA P49768 S43-p Phosphorylation QEHNDRRsLGHPEPL P49768 S51-p Phosphorylation LGHPEPLsNGRPQGN P49768 S59-p Phosphorylation NGRPQGNsRQVVEQD P49768 T74-p Phosphorylation EEEDEELtLKYGAKH P49768 S102-p Phosphorylation VVVATIKsVsFYTRk P49768 S104-p Phosphorylation VATIKsVsFYTRkDG P49768 Y154-p Phosphorylation TILLVVLyKYRCYKV P49768 Y240-p Phosphorylation MALVFIKyLPEWTAW P49768 Y256-p Phosphorylation ILAVISVyDLVAVLC P49768 S310-p Phosphorylation PEAQRRVsKNskYNA P49768 S313-p Phosphorylation QRRVsKNskYNAEst P49768 S319-p Phosphorylation NskYNAEstEREsQD P49768 T320-p Phosphorylation skYNAEstEREsQDT P49768 S324-p Phosphorylation AEstEREsQDTVAEN P49768 S346-p Phosphorylation EWEAQRDsHLGPHRs P49768 S353-p Phosphorylation sHLGPHRstPEsRAA P49768 T354-p Phosphorylation HLGPHRstPEsRAAV P49768 S357-p Phosphorylation PHRstPEsRAAVQEL P49768 S365-p Phosphorylation RAAVQELsssILAGE P49768 S366-p Phosphorylation AAVQELsssILAGED P49768 S367-p Phosphorylation AVQELsssILAGEDP P49768 K109-ub Ubiquitination sVsFYTRkDGQLIYT P49768 K314-ub Ubiquitination RRVsKNskYNAEstE

PTM - dbPTM UniProt ID Position Code Type Resources References P49768 43 S Phosphorylation Swiss-Prot 1010711 17081983;18691976 P49768 310 S Phosphorylation Swiss-Prot 1010711 14576165 P49768 346 S Phosphorylation Swiss-Prot 1010711 14576165 P49768 365 S Phosphorylation Swiss-Prot 1010711 18691976 P49768 367 S Phosphorylation Swiss-Prot 1010711 18691976;19690332 P49768 43 S Phosphorylation PhosphoELM.10011 17081983 P49768 365 S Phosphorylation PhosphoELM.10011 18669648 P49768 366 S Phosphorylation PhosphoELM.10011 18669648 P49768 367 S Phosphorylation PhosphoELM.10011 18669648;17081983 P49768 353 S Phosphorylation PhosphoELM.10011 11104755 P49768 357 S Phosphorylation PhosphoELM.10011 11104755 P49768 310 S Phosphorylation PhosphoELM.10011 14576165 P49768 346 S Phosphorylation PhosphoELM.10011 14576165 P49768 354 T Phosphorylation PhosphoELM.10011 12056836;12056836 P49768 109 K Ubiquitylation Phosphositeplus.1010730 21906983 P49768 314 K Ubiquitylation Phosphositeplus.1010730 21906983 P49768 310 S Phosphorylation Phosphositeplus.1010730 14576165 P49768 319 S Phosphorylation Phosphositeplus.1010730 18667537 P49768 346 S Phosphorylation Phosphositeplus.1010730 14576165 P49768 353 S Phosphorylation Phosphositeplus.1010730 11104755;17360711;17389597 P49768 357 S Phosphorylation Phosphositeplus.1010730 11104755;17360711;17389597 P49768 365 S Phosphorylation Phosphositeplus.1010730 18691976;20058876;20363803;21659604 P49768 366 S Phosphorylation Phosphositeplus.1010730 20363803;20815410;21406692;22115753 P49768 367 S Phosphorylation Phosphositeplus.1010730 18578522;18691976;19690332;20058876;20068231;20363803;20815410;21406692;21659604 P49768 43 S Phosphorylation Phosphositeplus.1010730 18578522;18691976;20068231;21406692 P49768 51 S Phosphorylation Phosphositeplus.1010730 20068231 P49768 320 T Phosphorylation Phosphositeplus.1010730 18667537 P49768 354 T Phosphorylation Phosphositeplus.1010730 12056836;20456003 P49768 43 S Phosphorylation HPRD 9.0 17081983,18691976,18578522,20068231 P49768 51 S Phosphorylation HPRD 9.0 20068231 P49768 59 S Phosphorylation HPRD 9.0 20068231 P49768 365 S Phosphorylation HPRD 9.0 17081983,18669648,18691976,19651622,20068231,20058876 P49768 366 S Phosphorylation HPRD 9.0 17081983,18669648,18691976,19651622,20068231 P49768 367 S Phosphorylation HPRD 9.0 15345747,18669648,18691976,19651622,18578522,20068231,20058876 P49768 354 T Phosphorylation (CDK5) HPRD 9.0 12056836 P49768 353 S Phosphorylation (GSK3B) HPRD 9.0 11104755 P49768 357 S Phosphorylation (GSK3B) HPRD 9.0 11104755 P49768 310 S Phosphorylation (PRKACA) HPRD 9.0 14576165 P49768 346 S Phosphorylation (PRKCZ) HPRD 9.0 14576165 P49768 345 D Proteolytic Cleavage HPRD 9.0 10069390 P49768 345 D Proteolytic Cleavage HPRD 9.0 10069390 P49768 333 D Proteolytic Cleavage HPRD 9.0 10069390 P49768 345 D Proteolytic Cleavage HPRD 9.0 10069390 P49768 333 D Proteolytic Cleavage HPRD 9.0 10069390 P49768 333 D Proteolytic Cleavage HPRD 9.0 10069390 P49768 298 M Proteolytic Cleavage HPRD 9.0 9173929 P49768 43 S Phosphorylation SysPTM 1.1 17081983;18691976 P49768 365 S Phosphorylation SysPTM 1.1 17081983;18691976 P49768 366 S Phosphorylation SysPTM 1.1 17081983 P49768 367 S Phosphorylation SysPTM 1.1 17081983;18691976 P49768 354 T Phosphorylation SysPTM 1.1 17081983

PTM - HPRD Symbol Position Code Type References PSEN1 298 M Proteolytic Cleavage 9173929 PSEN1 310 S Phosphorylation 14576165 PSEN1 333 D Proteolytic Cleavage 10069390 PSEN1 345 D Proteolytic Cleavage 10069390 PSEN1 346 S Phosphorylation 14576165 PSEN1 350 T Phosphorylation 17081983 PSEN1 353 S Phosphorylation 11104755 PSEN1 354 T Phosphorylation 12056836 PSEN1 357 S Phosphorylation 11104755 PSEN1 361 S Phosphorylation 17081983,18669648,18691976,19651622,20068231,20058876 PSEN1 362 S Phosphorylation 17081983,18669648,18691976,19651622,20068231 PSEN1 363 S Phosphorylation 17081983,18669648,18691976,19651622,18578522,20068231,20058876 PSEN1 363 S Phosphorylation 20068231 PSEN1 365 S Phosphorylation 17081983,18669648,18691976,19651622,20068231,20058876 PSEN1 366 S Phosphorylation 17081983,18669648,18691976,19651622,20068231 PSEN1 367 S Phosphorylation 15345747,18669648,18691976,19651622,18578522,20068231,20058876 PSEN1 39 S Phosphorylation 17081983,18691976,18578522,20068231 PSEN1 43 S Phosphorylation 17081983,18691976,18578522,20068231 PSEN1 47 S Phosphorylation 20068231 PSEN1 51 S Phosphorylation 20068231 PSEN1 55 S Phosphorylation 20068231 PSEN1 59 S Phosphorylation 20068231

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type P49768 357 S 11104755 GSK-3_beta LTP Phosphorylation P49768 43 S 17081983 HTP Phosphorylation P49768 366 S 18669648 HTP Phosphorylation P49768 346 S 14576165 PKC_alpha LTP Phosphorylation P49768 353 S 11104755 GSK-3_beta LTP Phosphorylation P49768 367 S 18669648 HTP Phosphorylation P49768 367 S 17081983 HTP Phosphorylation P49768 310 S 14576165 PKA_group LTP Phosphorylation P49768 354 T 12056836 CDK_group LTP Phosphorylation P49768 354 T 12056836 CDK5 LTP Phosphorylation P49768 365 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs P49768 43 Phosphoserine 17081983;21406692;23186163 P49768 310 Phosphoserine; by PKA 14576165 P49768 346 Phosphoserine; by PKC 14576165 P49768 367 Phosphoserine 21406692;23186163

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type PSEN1 IPI00028077 S 365 AAVQELpSSSILAG phosphorylation PSEN1 IPI00028077 S 367 VQELSSpSILAGED phosphorylation PSEN1 IPI00028077 S 43 EHNDRRpSLGHPEP phosphorylation PSEN1 IPI00028077 S 51 GHPEPLpSNGRPQG phosphorylation PSEN1 IPI00028077 S 59 GRPQGNpSRQVVEQ phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 5663 111642 - K Ubiquitination 21139048 5663 111642 - K Ubiquitination 21906983 5663 111642 - K Ubiquitination 23000965 5663 111642 - K Ubiquitination 23707529 5663 111642 - K Ubiquitination 10527805 5663 111642 - K Ubiquitination 24816145 5663 111642 - K Ubiquitination 24399296

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID PSEN1 Homo sapiens (Human) 109 "109,VSFYTRKDGQLIY" 109:21906983 PSEN1 Homo sapiens (Human) 109 "109,VSFYTRKDGQLIY" 109:21906983 PSEN1 Homo sapiens (Human) 109; 314 "109,VSFYTRKDGQLIY; 314,RVSKNSKYNAEST" 109:21906983; 314:21906983 PSEN1 Homo sapiens (Human) 310; 105 "310,RVSKNSKYNAEST; 105,VSFYTRKDGQLIY" 310:21906983; 105:21906983 PSEN1 Homo sapiens (Human) 105 "105,VSFYTRKDGQLIY" 105:21906983 PSEN1 Homo sapiens (Human) 109 "109,VSFYTRKDGQLIY" 109:21906983