PPI - IID Symbol 1 Symbol 2 PMID DBs Evidence Type Adipose Tissue Adrenal Gland Amygdala Bone Bone Marrow Brain Dorsal Root Ganglia Eye Heart Hindgut Hypothalamus Kidney Liver Lung Lymph Nodes Mammary Gland Midgut Neurons Ovary Pancreas Pituitary Gland Placenta Prostate Salivary Gland Skeletal Muscle Small Intestine Spleen Stomach Testes Uterus ZNF426 USP25 25416956 biogrid,intact exp GP GP GP G GP GP G GP P GP GP G GP GP GP P GP GP P G GP GP ANXA1 USP25 19615732 biogrid,intact exp GP G G G GP GP G G GP G GP G GP GP G G GP G G G GP GP GP G G GP G G G KLHL13 USP25 19615732 biogrid exp GP G G G G GP GP G GP GP G G GP G G GP G GP GP GP GP GP GP G CUL2 USP25 21145461 biogrid exp GP GP G G G GP G G GP G GP GP GP GP G G GP GP GP G GP GP GP G G G GP GP G KRT4 USP25 19615732 biogrid,intact exp GP GP G G G G G HSP90B1 USP25 22863883 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G MAGEB4 USP25 25416956 biogrid,intact exp P P P P P P P P P P P P P P P P P P P P BTBD9 USP25 19615732 biogrid,intact exp P G G G GP GP G G GP G G G GP GP G G GP GP G G GP G G GP GP GP G GAPDH USP25 16501887 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G SYK USP25 25416956,19909739,21516116 biogrid,mint,intact exp GP G G G GP GP G GP G GP G GP GP G GP GP GP G G G G G GP GP GP GP G MYBPC1 USP25 16501887 biogrid exp G G G G G G G G G G G G GP G G G KCTD13 USP25 19615732 biogrid,intact exp GP GP G G G GP G GP GP G GP GP GP G G GP GP GP GP G GP GP GP GP GP G GP GP G KLHL9 USP25 19615732 biogrid,intact exp GP G G G G G G GP GP G GP GP G G G GP G G GP G G GP GP GP GP G GP GP G FLNC USP25 16501887 biogrid exp G GP G G G GP G GP GP G GP G GP G G GP GP G GP G GP G GP GP GP G GP GP G SON USP25 22939629 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G TNKS2 USP25 22153077 intact exp G G G G G G G G G G GP G G G G G G G GP G G G G G G G G G G MEF2C USP25 20936779 i2d exp G GP G G GP GP G GP GP G GP G GP GP G G GP GP G G G G GP G GP G GP G ZG16B USP25 19615732 biogrid,intact exp G G P G G G GP GP G G GP SENP1 USP25 18538659 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G USP25 MYO6 19615732 biogrid,intact exp GP GP G GP G G GP G GP GP GP G G GP GP G GP G GP GP GP P GP G GP GP PIP USP25 19615732 biogrid,intact exp G G G G G G G G G G G G G G G G G G GP G G G G CDC37L1 USP25 22863883 biogrid exp GP GP G G GP GP G G GP G GP GP GP G G GP GP G GP G GP GP GP GP GP G GP GP G SUMO2 USP25 25416956,18538659 biogrid,intact exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G KIAA1468 USP25 22863883 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G LYZ USP25 19615732 biogrid,intact exp G G G GP G G G GP G GP GP G GP G G G G G GP GP GP GP G SKIL USP25 15231748 hprd,biogrid,i2d,mint,intact exp GP GP G G GP GP G G GP G GP GP GP G G GP GP G G G GP GP GP GP GP G GP GP G SUMO1 USP25 18538659 biogrid exp GP GP G G GP GP G GP GP G GP G GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G USP25 CROT 26186194 biogrid exp GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G MT2A USP25 16501887 biogrid exp G G G G G G G G G G G G G G G G G G G G G G G G G G G G G RAD23A USP25 19013454,25416956 biogrid,intact exp, ortho GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G SUMO3 USP25 18538659 biogrid exp, ortho GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G KCTD10 USP25 19615732 biogrid,intact exp, ortho G GP G G GP GP G GP GP G GP G GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G USP25 USP25 19440361 biogrid exp, ortho GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G CUL3 USP25 21145461 biogrid exp, ortho GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G TRAF5 USP25 23042150 intact exp, ortho GP GP G G GP GP G GP GP G GP GP GP GP G GP GP G GP G GP GP GP GP GP GP GP GP G WRNIP1 USP25 19615732 biogrid,intact exp, ortho GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G USP25 TRAF6 23042150 intact exp, ortho GP GP G G GP G G GP GP G GP GP GP G G GP G GP G G GP GP GP G GP GP GP GP G APP USP25 25740315 biogrid exp, ortho G GP G G GP GP G G GP G G G G G G GP GP G GP G GP GP G G GP G G GP G UBE2I USP25 20936779,18538659 biogrid,i2d exp, ortho GP GP G G GP GP G GP GP G GP G GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G USP28 USP25 19615732 biogrid,intact exp, ortho GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G UBC USP25 23314748,22195557,18538659 biogrid exp, ortho GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G NEDD8 USP25 19615732, 16082366 biogrid,intact exp, ortho, pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G ACTA1 USP25 16501887, 25402006 biogrid exp, pred G G G G G GP G G G G G G G G GP G G G FGD4 USP25 25402006 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP G GP GP GP GP G MARK3 USP25 16082366 pred GP GP G G GP GP G G GP G GP G GP GP G GP GP G GP G GP GP GP G GP G GP GP G MARK2 USP25 16082366 pred P P G G G GP GP GP G P P P G GP GP P G GP GP GP P P GP GP P USP25 FHOD1 25402006 pred G GP G G GP G G G GP G G G GP GP G G G GP G G G G G GP G G G GP G CNN1 USP25 25402006 pred G G G G G G G G G G G G G G G G G G G G G G LATS2 USP25 16082366 pred GP GP G G GP GP G G GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G VIL1 USP25 25402006 pred P GP GP G GP P G G GP G UBE2K USP25 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G GSN USP25 25402006 pred GP GP G G GP GP G G GP G GP G GP GP G G GP GP GP G GP G GP G G GP GP G G USP13 USP25 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G LATS1 USP25 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP G GP GP G USP14 USP25 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP GP GP GP GP GP GP G TMSB4X USP25 25402006 pred G GP G G GP GP G G GP G GP G GP GP G G GP G GP G G G G GP G GP GP GP G USP5 USP25 16082366 pred GP GP G G GP GP G GP GP G GP GP GP GP G GP GP GP GP G GP GP G GP GP GP GP GP G DNASE1 USP25 25402006 pred G G P GP G G GP P P G G GP G GP UBQLN3 USP25 16082366 pred G G G G G G GP G GC USP25 25402006 pred P P P GP GP GP P GP P P GP GP P CFL1 USP25 25402006 pred GP GP G G GP GP G GP GP G GP GP GP G G GP GP G GP G GP GP GP GP GP GP GP GP G MARK1 USP25 16082366 pred GP GP G G GP GP G GP GP G GP P GP GP G GP GP G GP G GP GP GP GP GP GP GP GP G OCLN USP25 25402006 pred GP G G G G GP G G GP G GP GP GP G G GP GP GP GP G GP GP GP G GP G GP GP G COTL1 USP25 25402006 pred G G G G GP GP G P G G GP GP GP GP G G GP G GP G GP G P G GP GP G G TPM3 USP25 25402006 pred G P GP G GP G G GP G GP G G G SLAMF7 USP25 21836163 pred G G GP G G GP GP G G G G G G G G

PPI - iRefIndex UniProt A UniProt B Method PMID Interaction Type Edge type Num Participants P04406 Q9UHP3 two hybrid 16501887 direct interaction X 2 P12273 Q9UHP3 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 A0A024R7G8 affinity chromatography technology 19013454 physical association X 2 Q9UHP3 A0A024R244 two hybrid 19909739 direct interaction X 2 Q9UHP3 A0A024R244 two hybrid 19909739 direct interaction X 2 Q9UHP3 A0A024R244 affinity chromatography technology 19909739 physical association X 2 Q9UHP3 A0A024R244 affinity chromatography technology 19909739 physical association X 2 Q9UHP3 A0A024R3Z2 pull down 18538659 direct interaction X 2 Q9UHP3 P55854 pull down 18538659 direct interaction X 2 Q9UHP3 A0A024R3Z2 pull down 18538659 direct interaction X 2 Q9UHP3 P55854 pull down 18538659 direct interaction X 2 Q9UHP3 P61956-2 pull down 18538659 direct interaction X 2 Q9UHP3 A0A024R475 affinity chromatography technology 21145461 physical association X 2 Q9UHP3 Q13617 affinity chromatography technology 21145461 physical association X 2 Q9UHP3 P18583-6 biochemical 22939629 colocalization X 2 Q9UHP3 Q9P260 biochemical 22863883 colocalization X 2 Q9UHP3 Q7L3B6 biochemical 22863883 colocalization X 2 Q9UHP3 P14625 biochemical 22863883 colocalization X 2 Q9UHP3 P55854 affinity chromatography technology 18538659 physical association X 2 Q9UHP3 A0A024R3Z2 affinity chromatography technology 18538659 physical association X 2 Q9UHP3 P55854 affinity chromatography technology 18538659 physical association X 2 Q9UHP3 P55854 affinity chromatography technology 18538659 physical association X 2 Q9UHP3 A0A024R7G8 two hybrid 25416956 direct interaction X 2 Q9UHP3 P61956-2 two hybrid 25416956 direct interaction X 2 Q9UHP3 A0A024R244 two hybrid 25416956 direct interaction X 2 Q9UHP3 A0A024R3Z2 pull down 19440361 direct interaction X 2 Q9UHP3 P61956-2 pull down 19440361 direct interaction X 2 Q9UHP3 P04083 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 Q96S55-2 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 Q96RU2 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 A0A024RBJ2 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 Q96Q07-3 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 Q96HC9 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 B3KPP3 two hybrid 16501887 direct interaction X 2 Q9UHP3 B3KPP3 affinity chromatography technology 16501887 physical association X 2 Q9UHP3 Q9UHP3 affinity chromatography technology 19440361 physical association X 2 P02795 Q9UHP3 two hybrid 16501887 direct interaction X 2 O15481 Q9UHP3 two hybrid 25416956 direct interaction X 2 G8H6I3 Q9UHP3 affinity chromatography technology 19615732 physical association X 2 P68133 Q9UHP3 two hybrid 16501887 direct interaction X 2 P68133 Q9UHP3 pull down 16501887 direct interaction X 2 Q9UHP3 P63279 enzymatic study 18538659 direct interaction X 2 Q9UHP3 P12757-5 two hybrid 15231748 direct interaction X 2 Q9UHP3 P63279 pull down 18538659 direct interaction X 2 Q9UHP3 Q9P0U3-2 enzymatic study 18538659 direct interaction X 2 Q9UHP3 A0A024R7D7 two hybrid 25416956 direct interaction X 2 Q9UHP3 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 B2R4C5 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 Q9UM54-1 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 Q15843 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 Q8WZ19 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 Q14315 two hybrid 16501887 direct interaction X 2 P0CG48 Q9UHP3 enzymatic study 22195557 direct interaction X 2 P0CG48 Q9UHP3 affinity chromatography technology 22053931 physical association X 2 P0CG48 Q9UHP3 pull down 23314748 direct interaction X 2 P0CG48 Q9UHP3 affinity chromatography technology 23000965 physical association X 2 P0CG48 Q9UHP3 affinity chromatography technology 25015289 physical association X 2 P0CG48 Q9UHP3 enzymatic study 18538659 direct interaction X 2 P0CG48 Q9UHP3 pull down 18538659 direct interaction X 2 P0CG48 Q9UHP3 affinity chromatography technology 19440361 physical association X 2 P0CG48 Q9UHP3 affinity chromatography technology 22118674 physical association X 2 P0CG48 Q9UHP3 affinity chromatography technology 20972266 physical association X 2 P0CG48 Q9UHP3 affinity chromatography technology 21963094 physical association X 2 P0CG48 Q9UHP3 affinity chromatography technology 21139048 physical association X 2 P0CG48 Q9UHP3 affinity chromatography technology 21890473 physical association X 2 P0CG48 Q9UHP3 affinity chromatography technology 21906983 physical association X 2 Q58EZ4 Q9UHP3 affinity chromatography technology 19615732 physical association X 2 Q9UHP3 P12757-3 two hybrid 15231748 - X 2 Q9UHP3 P12757-3 two hybrid 15231748 - X 2 Q9UHP3 anti tag coimmunoprecipitation 19615732 physical association C 14 Q9UHP3 A0A024R244 two hybrid 21516116 physical association X 2 Q9UHP3 Q9H2K2 fluorescence polarization spectroscopy 22153077 direct interaction X 2 Q9UHP3 O00463 anti bait coimmunoprecipitation 23042150 physical association X 2 Q9UHP3 A0A024R244 two hybrid prey pooling approach 25416956 physical association X 2 Q9UHP3 A0A024R7G8 two hybrid prey pooling approach 25416956 physical association X 2 Q9UHP3 A0A024R8S3 two hybrid prey pooling approach 25416956 physical association X 2 Q9UHP3 A0A024R7G8 two hybrid prey pooling approach 25416956 physical association X 2 Q9UHP3 A0A024R244 two hybrid array 25416956 physical association X 2 Q9UHP3 A0A024R7G8 two hybrid array 25416956 physical association X 2 Q9UHP3 A0A024R8S3 two hybrid array 25416956 physical association X 2 Q9UHP3 A0A024R7G8 two hybrid array 25416956 physical association X 2 Q9UHP3 A0A024R244 validated two hybrid 25416956 physical association X 2 Q9UHP3 A0A024R7G8 validated two hybrid 25416956 physical association X 2 Q9UHP3 A0A024R8S3 validated two hybrid 25416956 physical association X 2 O15481 Q9UHP3 two hybrid prey pooling approach 25416956 physical association X 2 O15481 Q9UHP3 two hybrid array 25416956 physical association X 2 O15481 Q9UHP3 validated two hybrid 25416956 physical association X 2 P12757 Q9UHP3 two hybrid 15231748 physical association X 2 Q9UHP3 Q9Y4K3 anti bait coimmunoprecipitation 23042150 physical association X 2 Q9UHP3 A0A024R7D7 two hybrid prey pooling approach 25416956 physical association X 2 Q9UHP3 A0A024R7D7 two hybrid array 25416956 physical association X 2 Q9UHP3 A0A024R7D7 validated two hybrid 25416956 physical association X 2

PPI - PINA Gene A Gene B Method Publications Species A Species B Interaction Type Source Database Interaction Identidiers Experimental Role(s) Interactor A Experimental Role(s) Interactor B CUL3 USP25 MI:0004(affinity chromatography technology) 21145461 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_202241 bait prey UBC USP25 MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0415(enzymatic study)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0415(enzymatic study)|MI:0004(affinity chromatography technology)|MI:0004(affinity chromatography technology)|MI:0096(pull down) 19440361,20972266,21139048,21890473,21906983,21963094,22053931,22118674,22195557,23000965,23314748 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction) (biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid)|(biogrid) BIOGRID_168510|BIOGRID_177883|BIOGRID_176042|BIOGRID_199465|BIOGRID_166140|BIOGRID_148426|BIOGRID_164232|BIOGRID_160559|BIOGRID_270449|BIOGRID_222056|BIOGRID_248007 bait|bait|bait|prey|bait|bait|bait|bait|bait|bait|bait prey|prey|prey|bait|prey|prey|prey|prey|prey|prey|prey USP25 MYO6 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100690|BIOGRID_119605 bait|bait prey|prey HSP90B1 USP25 MI:0401(biochemical) 22863883 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0403(colocalization) (biogrid) BIOGRID_304868 bait prey SUMO2 USP25 MI:0096(pull down) 18538659 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_197922 prey bait UBE2I USP25 MI:0096(pull down) 18538659 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_197921 bait prey BTBD9 USP25 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_120784|UniProt_100690 prey|prey bait|bait TRAF5 USP25 MI:0006(anti bait coimmunoprecipitation) 23042150 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (intact) UniProt_124308 bait prey ZG16B USP25 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100690|BIOGRID_120801 prey|prey bait|bait NEDD8 USP25 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_119615|UniProt_100690 prey|prey bait|bait FLNC USP25 MI:0018(two hybrid) 16501887 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_124665 prey bait SYK USP25 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid) 19909739,21516116 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association)|MI:0407(direct interaction)|MI:0915(physical association) (biogrid)|(biogrid)|(biogrid)|(biogrid)|(intact) BIOGRID_194543|BIOGRID_194542|BIOGRID_194544|BIOGRID_194540|UniProt_114515 prey|bait|bait|prey|bait bait|prey|prey|bait|prey KCTD13 USP25 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100690|BIOGRID_120858 prey|prey bait|bait CDC37L1 USP25 MI:0401(biochemical) 22863883 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0403(colocalization) (biogrid) BIOGRID_304867 bait prey KLHL13 USP25 MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_121752 bait prey GAPDH USP25 MI:0018(two hybrid) 16501887 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_124662 prey bait PIP USP25 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_119649|UniProt_100690 prey|prey bait|bait WRNIP1 USP25 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_120582|UniProt_100690 prey|prey bait|bait ACTA1 USP25 MI:0018(two hybrid)|MI:0018(two hybrid) 16501887 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0407(direct interaction) (biogrid)|(biogrid) BIOGRID_124667|BIOGRID_124664 prey|prey bait|bait ANXA1 USP25 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_119346|UniProt_100690 prey|prey bait|bait RAD23A USP25 MI:0415(enzymatic study) 19013454 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_188745 bait prey KIAA1468 USP25 MI:0401(biochemical) 22863883 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0403(colocalization) (biogrid) BIOGRID_300757 prey bait SKIL USP25 MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid)|MI:0018(two hybrid) 15231748 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association)|MI:0407(direct interaction)|-|MI:0915(physical association) MI:0471(mint)|MI:0471(mint)|(biogrid)|MI:0468(hprd)|(intact) MINT_31812|MINT_60603|BIOGRID_258985|HPRD_38427|UniProt_78687 bait|bait|bait|-|bait prey|prey|prey|-|prey CUL2 USP25 MI:0004(affinity chromatography technology) 21145461 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (biogrid) BIOGRID_204064 bait prey KCTD10 USP25 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_120711|UniProt_100690 prey|prey bait|bait USP25 TRAF6 MI:0006(anti bait coimmunoprecipitation) 23042150 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association) (intact) UniProt_124307 prey bait KRT4 USP25 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_119544|UniProt_100690 prey|prey bait|bait SUMO1 USP25 MI:0096(pull down)|MI:0096(pull down) 18538659 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0407(direct interaction) (biogrid)|(biogrid) BIOGRID_197917|BIOGRID_197919 bait|prey prey|bait KLHL9 USP25 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_120569|UniProt_100690 prey|prey bait|bait MYBPC1 USP25 MI:0018(two hybrid)|MI:0018(two hybrid) 16501887 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0407(direct interaction) (biogrid)|(biogrid) BIOGRID_124666|BIOGRID_124663 prey|prey bait|bait SON USP25 MI:0401(biochemical) 22939629 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0403(colocalization) (biogrid) BIOGRID_241495 bait prey TNKS2 USP25 MI:0053(fluorescence polarization spectroscopy) 22153077 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (intact) UniProt_121609 neutral component neutral component SUMO3 USP25 MI:0096(pull down)|MI:0096(pull down) 18538659 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction)|MI:0407(direct interaction) (biogrid)|(biogrid) BIOGRID_197920|BIOGRID_197918 prey|bait bait|prey USP28 USP25 MI:0004(affinity chromatography technology)|MI:0007(anti tag coimmunoprecipitation) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (biogrid)|(intact) BIOGRID_120611|UniProt_100690 prey|prey bait|bait MT2A USP25 MI:0018(two hybrid) 16501887 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0407(direct interaction) (biogrid) BIOGRID_124661 prey bait LYZ USP25 MI:0007(anti tag coimmunoprecipitation)|MI:0004(affinity chromatography technology) 19615732 taxid:9606(Homo sapiens) taxid:9606(Homo sapiens) MI:0915(physical association)|MI:0915(physical association) (intact)|(biogrid) UniProt_100690|BIOGRID_119572 prey|prey bait|bait

PPI - HINT Gene A Gene B PMID Method Quality B4DRS2 USP25 19615732 0004 HT EECP USP25 19615732 0004 HT ANXA1 USP25 19615732,19615732 0004,0007 HT UBC USP25 18538659,20972266,21963094,23314748,19440361,18538659,22118674 0096,0004,0004,0096,0096,0415,0004 LC PIP USP25 19615732,19615732 0004,0007 HT KRT4 USP25 19615732 0007 HT LYZ USP25 19615732,19615732 0004,0007 HT CUL2 USP25 21145461 0004 HT CUL3 USP25 21145461 0004 HT NEDD8 USP25 19615732,19615732 0004,0007 HT Q6ZRV7 USP25 22939629 0401 HT CDC37L1 USP25 22863883 0401 HT KCTD13 USP25 19615732,19615732 0004,0007 HT ZG16B USP25 19615732,19615732 0004,0007 HT BTBD9 USP25 19615732,19615732 0004,0007 HT USP28 USP25 19615732,19615732 0004,0007 HT WRNIP1 USP25 19615732,19615732 0004,0007 HT KCTD10 USP25 19615732,19615732 0004,0007 HT KIAA1468 USP25 22863883 0401 HT KLHL9 USP25 19615732,19615732 0004,0007 HT KLHL13 USP25 19615732 0004 HT USP25 CROT 26186194 0004 HT USP25 MYO6 19615732,19615732 0004,0007 HT USP25 HEL-S-125M 22863883 0401 HT B4DRS2 USP25 19615732 0004 HT EECP USP25 19615732 0004 HT ANXA1 USP25 19615732,19615732 0004,0007 HT PIP USP25 19615732,19615732 0004,0007 HT KRT4 USP25 19615732 0007 HT LYZ USP25 19615732,19615732 0004,0007 HT CUL2 USP25 21145461 0004 HT CUL3 USP25 21145461 0004 HT NEDD8 USP25 19615732,19615732 0004,0007 HT Q6ZRV7 USP25 22939629 0401 HT CDC37L1 USP25 22863883 0401 HT KCTD13 USP25 19615732,19615732 0004,0007 HT ZG16B USP25 19615732,19615732 0004,0007 HT BTBD9 USP25 19615732,19615732 0004,0007 HT USP28 USP25 19615732,19615732 0004,0007 HT WRNIP1 USP25 19615732,19615732 0004,0007 HT KCTD10 USP25 19615732,19615732 0004,0007 HT KIAA1468 USP25 22863883 0401 HT KLHL9 USP25 19615732,19615732 0004,0007 HT KLHL13 USP25 19615732 0004 HT USP25 CROT 26186194 0004 HT USP25 MYO6 19615732,19615732 0004,0007 HT USP25 HEL-S-125M 22863883 0401 HT UBC USP25 18538659,20972266,21963094,23314748,19440361,18538659,22118674 0096,0004,0004,0096,0096,0415,0004 LC B3KPP3 USP25 16501887,16501887 0004,0018 LC MAGEB4 USP25 25416956,25416956,25416956,25416956 1112,0397,0018,1356 HT MT2A USP25 16501887 0018 LC GAPDH USP25 16501887 0018 LC UBC USP25 18538659,22195557,18538659 0096,0415,0415 LC SKIL USP25 15231748 0018 HT SYK USP25 21516116,25416956,25416956,25416956,25416956,19909739,19909739 0018,1112,0397,0018,1356,0004,0018 HT,LC RAD23A USP25 25416956,25416956,25416956,25416956 1112,0397,0018,1356 HT SUMO2 USP25 25416956,25416956,25416956,25416956 1112,0397,0018,1356 HT UBE2I USP25 18538659,18538659 0096,0415 LC ACTA1 USP25 16501887,16501887 0018,0096 LC FLNC USP25 16501887 0018 LC Q59H94 USP25 16501887 0018 LC Q6ZRV7 USP25 22939629 0401 LC CDC37L1 USP25 22863883 0401 LC DKFZP451M091 USP25 16501887,16501887 0004,0018 LC ZNF426 USP25 25416956,25416956,25416956,25416956 1112,0397,0018,1356 HT TNKS2 USP25 22153077 0053 LC SENP1 USP25 18538659 0415 LC KIAA1468 USP25 22863883 0401 LC USP25 HEL-S-162EP 16501887 0018 LC USP25 HEL-S-125M 22863883 0401 LC

PPI - Mentha Protein A Protein B Gene A Gene B Score PMID Q9UHP3 P0CG48 USP25 UBC 0.731 22195557,23314748,18538659 Q9UHP3 Q7L3B6 USP25 CDC37L1 0.081 22863883 Q9UHP3 Q9P2J3 USP25 KLHL9 0.236 19615732 Q9UHP3 P04083 USP25 ANXA1 0.236 19615732 Q9UHP3 O15481 USP25 MAGEB4 0.454 25416956 Q9UHP3 Q9H3F6 USP25 KCTD10 0.236 19615732 Q9UHP3 Q00872 USP25 MYBPC1 0.454 16501887 Q9UHP3 P12273 USP25 PIP 0.236 19615732 Q9UHP3 P68133 USP25 ACTA1 0.523 16501887 Q9UHP3 P61626 USP25 LYZ 0.236 19615732 Q9UHP3 Q9BUY5 USP25 ZNF426 0.454 25416956 Q9UHP3 Q96DA0 USP25 ZG16B 0.236 19615732 Q9UHP3 Q15843 USP25 NEDD8 0.236 19615732 Q9UHP3 Q8WZ19 USP25 KCTD13 0.236 19615732 Q9UHP3 Q96S55 USP25 WRNIP1 0.236 19615732 Q9UHP3 Q96Q07 USP25 BTBD9 0.236 19615732 Q9UHP3 Q96RU2 USP25 USP28 0.236 19615732 Q9UM54 Q9UHP3 MYO6 USP25 0.236 19615732 Q9UJV3 Q9UHP3 MID2 USP25 0.236 unassigned1304 Q9UHP3 Q9BTV4 USP25 TMEM43 0.236 unassigned1304 Q9UHP3 Q3MIT2 USP25 PUS10 0.236 unassigned1304 Q9UHP3 P54727 USP25 RAD23B 0.236 unassigned1304 Q9UHP3 P63279 USP25 UBE2I 0.523 18538659 Q9UHP3 P54725 USP25 RAD23A 0.681 25416956,19013454,unassigned1304 Q9UHP3 P61956 USP25 SUMO2 0.712 25416956,unassigned1304,18538659 Q9UHP3 P43405 USP25 SYK 0.917 25416956,unassigned1304,19909739,21516116 Q9UHP3 Q9P0U3 USP25 SENP1 0.309 18538659 Q9UHP3 Q9P2N7 USP25 KLHL13 0.126 19615732 Q9UHP3 P02795 USP25 MT2A 0.309 16501887 Q9UHP3 P04406 USP25 GAPDH 0.309 16501887 Q9UHP3 P05067 USP25 APP 0.21 25740315 Q9UHP3 P12757 USP25 SKIL 0.332 15231748 Q9UHP3 P19013 USP25 KRT4 0.126 19615732 Q9UHP3 P55854 USP25 SUMO3 0.523 18538659 Q9UHP3 P63165 USP25 SUMO1 0.523 18538659 Q9UHP3 Q14315 USP25 FLNC 0.309 16501887 Q9UHP3 Q9H2K2 USP25 TNKS2 0.309 22153077 Q9UHP3 Q9P260 USP25 KIAA1468 0.081 22863883 Q9UHP3 Q9UHP3 USP25 USP25 0.236 19440361 Q9UKG9 Q9UHP3 CROT USP25 0.126 26186194 Q9UHP3 P14625 USP25 HSP90B1 0.081 22863883 Q9UHP3 Q13617 USP25 CUL2 0.126 21145461 Q9UHP3 Q13618 USP25 CUL3 0.126 21145461 Q9UHP3 P18583 USP25 SON 0.081 22939629

PPI - InWeb_IM Gene A Gene B MI 1 Interaction 1 MI 2 Interaction 2 TRAF5 USP25 MI:0045 experimental interaction detection MI:0461 interaction database MAGEB4 USP25 MI:0045 experimental interaction detection MI:0461 interaction database DDX58 USP25 MI:0362 inference MI:1106 pathways database MT2A USP25 MI:0045 experimental interaction detection MI:0461 interaction database ANXA1 USP25 MI:0045 experimental interaction detection MI:0461 interaction database GAPDH USP25 MI:0045 experimental interaction detection MI:0461 interaction database APP USP25 MI:0045 experimental interaction detection MI:0461 interaction database UBC USP25 MI:0045 experimental interaction detection MI:0461 interaction database PIP USP25 MI:0045 experimental interaction detection MI:0461 interaction database SKIL USP25 MI:0045 experimental interaction detection MI:0461 interaction database KRT4 USP25 MI:0045 experimental interaction detection MI:0461 interaction database SYK USP25 MI:0045 experimental interaction detection MI:0461 interaction database RAD23A USP25 MI:0045 experimental interaction detection MI:0461 interaction database SUMO3 USP25 MI:0045 experimental interaction detection MI:0461 interaction database LYZ USP25 MI:0045 experimental interaction detection MI:0461 interaction database SUMO2 USP25 MI:0045 experimental interaction detection MI:0461 interaction database SUMO1 USP25 MI:0045 experimental interaction detection MI:0461 interaction database UBE2I USP25 MI:0045 experimental interaction detection MI:0461 interaction database ACTA1 USP25 MI:0045 experimental interaction detection MI:0461 interaction database MYBPC1 USP25 MI:0045 experimental interaction detection MI:0461 interaction database MAP3K1 USP25 MI:0362 inference MI:0461 interaction database CUL2 USP25 MI:0045 experimental interaction detection MI:0461 interaction database CUL3 USP25 MI:0045 experimental interaction detection MI:0461 interaction database FLNC USP25 MI:0045 experimental interaction detection MI:0461 interaction database NEDD8 USP25 MI:0045 experimental interaction detection MI:0461 interaction database KCTD13 USP25 MI:0045 experimental interaction detection MI:0461 interaction database ZG16B USP25 MI:0045 experimental interaction detection MI:0461 interaction database BTBD9 USP25 MI:0045 experimental interaction detection MI:0461 interaction database USP28 USP25 MI:0045 experimental interaction detection MI:0461 interaction database WRNIP1 USP25 MI:0045 experimental interaction detection MI:0461 interaction database ZNF426 USP25 MI:0045 experimental interaction detection MI:0461 interaction database TNKS2 USP25 MI:0045 experimental interaction detection MI:0461 interaction database KCTD10 USP25 MI:0045 experimental interaction detection MI:0461 interaction database SENP1 USP25 MI:0045 experimental interaction detection MI:0461 interaction database KLHL9 USP25 MI:0045 experimental interaction detection MI:0461 interaction database KLHL13 USP25 MI:0045 experimental interaction detection MI:0461 interaction database USP25 CROT MI:0045 experimental interaction detection MI:0461 interaction database USP25 MYO6 MI:0045 experimental interaction detection MI:0461 interaction database USP25 TRAF6 MI:0045 experimental interaction detection MI:0461 interaction database