PTM - CPLM Gene Name UniProt ID Type Position Code RNF20; BRE1A Q5VTR2 Acetylation 348 K RNF20; BRE1A Q5VTR2 Acetylation 242 K RNF20; BRE1A Q5VTR2 Ubiquitination 194 K RNF20; BRE1A Q5VTR2 Ubiquitination 242 K RNF20; BRE1A Q5VTR2 Ubiquitination 821 K RNF20; BRE1A Q5VTR2 Ubiquitination 762 K RNF20; BRE1A Q5VTR2 Ubiquitination 256 K RNF20; BRE1A Q5VTR2 Ubiquitination 376 K RNF20; BRE1A Q5VTR2 Ubiquitination 775 K

PTM - dbPAF Gene Name UniProt ID Position Type Species RNF20; BRE1A Q5VTR2 122 T Homo sapiens(Human) RNF20; BRE1A Q5VTR2 136 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 138 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 16 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 172 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 2 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 208 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 262 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 265 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 269 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 31 T Homo sapiens(Human) RNF20; BRE1A Q5VTR2 314 Y Homo sapiens(Human) RNF20; BRE1A Q5VTR2 317 T Homo sapiens(Human) RNF20; BRE1A Q5VTR2 34 T Homo sapiens(Human) RNF20; BRE1A Q5VTR2 41 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 42 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 43 T Homo sapiens(Human) RNF20; BRE1A Q5VTR2 506 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 515 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 517 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 522 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 524 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 525 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 526 T Homo sapiens(Human) RNF20; BRE1A Q5VTR2 540 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 544 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 545 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 547 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 548 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 550 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 553 S Homo sapiens(Human) RNF20; BRE1A Q5VTR2 650 Y Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q5VTR2 K348-ac Acetylation NRLCELEkLRQDFEE Q5VTR2 K808-ac Acetylation VVRKLEEkEHLLQSN Q5VTR2 K874-ac Acetylation ENSVTKEkDMFNFkR Q5VTR2 K880-ac Acetylation EkDMFNFkRAQEDIS Q5VTR2 K610-m1 Methylation ERDSAKDkEKGkHDD Q5VTR2 K614-m1 Methylation AKDkEKGkHDDGRKK Q5VTR2 K769-m1 Methylation kLMSERIkSNQIHKL Q5VTR2 K880-m1 Methylation EkDMFNFkRAQEDIS Q5VTR2 T34-p Phosphorylation DSGTTVEtIKLGGVs Q5VTR2 S41-p Phosphorylation tIKLGGVssTEELDI Q5VTR2 S42-p Phosphorylation IKLGGVssTEELDIR Q5VTR2 T122-p Phosphorylation QGLGDLLtERKALVV Q5VTR2 S136-p Phosphorylation VPEPEPDsDsNQERK Q5VTR2 S138-p Phosphorylation EPEPDsDsNQERKDD Q5VTR2 S172-p Phosphorylation SSSEEMEsQLQERVE Q5VTR2 S262-p Phosphorylation SKVETAEsRVsVLEs Q5VTR2 S265-p Phosphorylation ETAEsRVsVLEsMID Q5VTR2 S269-p Phosphorylation sRVsVLEsMIDDLQW Q5VTR2 Y314-p Phosphorylation YGAGSSLyGGTITIN Q5VTR2 T444-p Phosphorylation EVIQLEDtLAQVRKE Q5VTR2 S515-p Phosphorylation LNKTRLRsGsALLQs Q5VTR2 S517-p Phosphorylation KTRLRsGsALLQsQs Q5VTR2 S522-p Phosphorylation sGsALLQsQsstEDP Q5VTR2 S524-p Phosphorylation sALLQsQsstEDPKD Q5VTR2 S525-p Phosphorylation ALLQsQsstEDPKDE Q5VTR2 T526-p Phosphorylation LLQsQsstEDPKDEP Q5VTR2 S540-p Phosphorylation PAELKPDsEDLSsQS Q5VTR2 S545-p Phosphorylation PDsEDLSsQSsAsKA Q5VTR2 S548-p Phosphorylation EDLSsQSsAsKAsQE Q5VTR2 S550-p Phosphorylation LSsQSsAsKAsQEDA Q5VTR2 S553-p Phosphorylation QSsAsKAsQEDANEI Q5VTR2 Y650-p Phosphorylation MKLLLDMyRSAPKEQ Q5VTR2 K324-sm Sumoylation TITINARkFEEMNAE Q5VTR2 K778-sm Sumoylation NQIHKLLkEEKEELA Q5VTR2 K242-ub Ubiquitination LTDLLQEkHRTMSQE Q5VTR2 K376-ub Ubiquitination SAVEQVVkETPEYRC Q5VTR2 K762-ub Ubiquitination EKDDANFkLMSERIk Q5VTR2 K821-ub Ubiquitination SNIGTGEkELGLRTQ

PTM - dbPTM UniProt ID Position Code Type Resources References Q5VTR2 136 S Phosphorylation Swiss-Prot 1010711 18669648;19413330 Q5VTR2 138 S Phosphorylation Swiss-Prot 1010711 15302935;18669648;19413330;19690332 Q5VTR2 314 Y Phosphorylation Swiss-Prot 1010711 18083107 Q5VTR2 348 K Acetylation Swiss-Prot 1010711 19608861 Q5VTR2 540 S Phosphorylation Swiss-Prot 1010711 17525332 Q5VTR2 545 S Phosphorylation Swiss-Prot 1010711 17525332 Q5VTR2 553 S Phosphorylation Swiss-Prot 1010711 17525332 Q5VTR2 136 S Phosphorylation PhosphoELM.10011 18669648 Q5VTR2 138 S Phosphorylation PhosphoELM.10011 18669648;17081983;15302935 Q5VTR2 522 S Phosphorylation PhosphoELM.10011 18669648 Q5VTR2 524 S Phosphorylation PhosphoELM.10011 18669648 Q5VTR2 525 S Phosphorylation PhosphoELM.10011 18669648 Q5VTR2 540 S Phosphorylation PhosphoELM.10011 17525332 Q5VTR2 545 S Phosphorylation PhosphoELM.10011 17525332 Q5VTR2 553 S Phosphorylation PhosphoELM.10011 17525332 Q5VTR2 526 T Phosphorylation PhosphoELM.10011 18669648 Q5VTR2 314 Y Phosphorylation PhosphoELM.10011 18083107 Q5VTR2 242 K Ubiquitylation Phosphositeplus.1010730 21890473 Q5VTR2 376 K Ubiquitylation Phosphositeplus.1010730 21906983 Q5VTR2 821 K Ubiquitylation Phosphositeplus.1010730 21890473 Q5VTR2 348 K Acetylation Phosphositeplus.1010730 19608861 Q5VTR2 136 S Phosphorylation Phosphositeplus.1010730 18491316;18669648;19664995;19690332;20058876;20164059;20815410;21659604;22115753 Q5VTR2 138 S Phosphorylation Phosphositeplus.1010730 15302935;18669648;19276368;19362540;19415658;19664994;19664995;19690332;20044836;20058876;20068231;20164059;20363803;20562096;20815410;21406692;21659604;22115753 Q5VTR2 172 S Phosphorylation Phosphositeplus.1010730 21362549 Q5VTR2 522 S Phosphorylation Phosphositeplus.1010730 17478428;18669648 Q5VTR2 524 S Phosphorylation Phosphositeplus.1010730 19276368 Q5VTR2 525 S Phosphorylation Phosphositeplus.1010730 19276368;21406692 Q5VTR2 540 S Phosphorylation Phosphositeplus.1010730 17525332 Q5VTR2 545 S Phosphorylation Phosphositeplus.1010730 17478428;17525332 Q5VTR2 548 S Phosphorylation Phosphositeplus.1010730 20044836 Q5VTR2 550 S Phosphorylation Phosphositeplus.1010730 20044836 Q5VTR2 553 S Phosphorylation Phosphositeplus.1010730 17525332;21362549 Q5VTR2 122 T Phosphorylation Phosphositeplus.1010730 20068231 Q5VTR2 314 Y Phosphorylation Phosphositeplus.1010730 18083107 Q5VTR2 348 K Acetylation HPRD 9.0 19608861 Q5VTR2 2 S Phosphorylation HPRD 9.0 20068231 Q5VTR2 16 S Phosphorylation HPRD 9.0 20068231 Q5VTR2 136 S Phosphorylation HPRD 9.0 17081983,18669648,19413330,18491316,19664995,18212344,19651622,18452278,20230923,20068231,20058876 Q5VTR2 138 S Phosphorylation HPRD 9.0 17322306,18669648,19413330,19415658,19664995,19651622,19664994,18452278,18767875,20230923,20068231,20058876 Q5VTR2 522 S Phosphorylation HPRD 9.0 18669648,20068231 Q5VTR2 524 S Phosphorylation HPRD 9.0 18669648,19651622,18452278 Q5VTR2 525 S Phosphorylation HPRD 9.0 18669648,19651622,18452278,20068231 Q5VTR2 540 S Phosphorylation HPRD 9.0 17525332 Q5VTR2 545 S Phosphorylation HPRD 9.0 17525332 Q5VTR2 553 S Phosphorylation HPRD 9.0 17525332 Q5VTR2 31 T Phosphorylation HPRD 9.0 20068231 Q5VTR2 34 T Phosphorylation HPRD 9.0 20068231 Q5VTR2 122 T Phosphorylation HPRD 9.0 20068231 Q5VTR2 317 T Phosphorylation HPRD 9.0 20068231 Q5VTR2 526 T Phosphorylation HPRD 9.0 18669648,18452278 Q5VTR2 138 S Phosphorylation SysPTM 1.1 17322306

PTM - HPRD Symbol Position Code Type References RNF20 122 T Phosphorylation 20068231 RNF20 136 S Phosphorylation 17081983,18669648,19413330,18491316,19664995,18212344,19651622,18452278,20230923,20068231,20058876 RNF20 138 S Phosphorylation 17322306,18669648,19413330,19415658,19664995,19651622,19664994,18452278,18767875,20230923,20068231,20058876 RNF20 16 S Phosphorylation 20068231 RNF20 2 S Phosphorylation 20068231 RNF20 31 T Phosphorylation 20068231 RNF20 317 T Phosphorylation 20068231 RNF20 34 T Phosphorylation 20068231 RNF20 348 K Acetylation 19608861 RNF20 522 S Phosphorylation 18669648,20068231 RNF20 524 S Phosphorylation 18669648,19651622,18452278 RNF20 525 S Phosphorylation 18669648,19651622,18452278,20068231 RNF20 526 T Phosphorylation 18669648,18452278 RNF20 540 S Phosphorylation 17525332 RNF20 545 S Phosphorylation 17525332 RNF20 553 S Phosphorylation 17525332

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q5VTR2 314 Y 18083107 HTP Phosphorylation Q5VTR2 525 S 18669648 HTP Phosphorylation Q5VTR2 553 S 17525332 HTP Phosphorylation Q5VTR2 524 S 18669648 HTP Phosphorylation Q5VTR2 522 S 18669648 HTP Phosphorylation Q5VTR2 136 S 18669648 HTP Phosphorylation Q5VTR2 545 S 17525332 HTP Phosphorylation Q5VTR2 526 T 18669648 HTP Phosphorylation Q5VTR2 138 S 18669648 HTP Phosphorylation Q5VTR2 138 S 17081983 HTP Phosphorylation Q5VTR2 138 S 15302935 HTP Phosphorylation Q5VTR2 540 S 17525332 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q5VTR2 41 Phosphoserine 23186163 Q5VTR2 136 Phosphoserine 19690332;23186163;24275569 Q5VTR2 138 Phosphoserine 19690332;20068231;21406692;23186163;24275569 Q5VTR2 348 N6-acetyllysine 19608861 Q5VTR2 522 Phosphoserine 18669648

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type RNF20 IPI00251559 K 350 RLCELEaKLRQDFE acetylation RNF20 IPI00251559 S 136 PEPEPDpSDSNQER phosphorylation RNF20 IPI00251559 S 138 PEPDSDpSNQERKD phosphorylation RNF20 IPI00251559 T 122 GLGDLLpTERKALV phosphorylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 56254 121119 - K Ubiquitination 21987572 56254 121119 - K Ubiquitination 21139048 56254 121119 - K Ubiquitination 21890473 56254 121119 - K Ubiquitination 21906983 56254 121119 - K Sumoylation 19471022 56254 121119 - K Ubiquitination 22053931 56254 121119 - K Ubiquitination 23000965 56254 121119 - K Ubiquitination 24816145 56254 121119 - K Ubiquitination 19850743 56254 121119 - K Ubiquitination 12938929 56254 121119 - K Neddylation 18247557

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID RNF20 Homo sapiens (Human) 823; 764; 196; 244 "823,NIGTGEKELGLRT; 764,KDDANFKLMSERI; 196,IVTVYDKLQEKVE; 244,TDLLQEKHRTMSQ" 823:21890473; 764:21890473; 196:21890473; 244:21890473 RNF20 Homo sapiens (Human) 823; 764; 196; 244; 378 "823,NIGTGEKELGLRT; 764,KDDANFKLMSERI; 196,IVTVYDKLQEKVE; 244,TDLLQEKHRTMSQ; 378,AVEQVVKETPEYR" 823:21890473; 764:21890473; 196:21890473; 244:21890473; 378:21906983 RNF20 Homo sapiens (Human) 823; 764; 196; 244 "823,NIGTGEKELGLRT; 764,KDDANFKLMSERI; 196,IVTVYDKLQEKVE; 244,TDLLQEKHRTMSQ" 823:21890473; 764:21890473; 196:21890473; 244:21890473