PTM - CPLM Gene Name UniProt ID Type Position Code OTUB1; OTB1; OTU1; HSPC263 Q96FW1 Acetylation 188 K OTUB1; OTB1; OTU1; HSPC263 Q96FW1 Ubiquitination 109 K OTUB1; OTB1; OTU1; HSPC263 Q96FW1 Ubiquitination 71 K OTUB1; OTB1; OTU1; HSPC263 Q96FW1 Ubiquitination 84 K OTUB1; OTB1; OTU1; HSPC263 Q96FW1 Ubiquitination 188 K OTUB1; OTB1; OTU1; HSPC263 Q96FW1 Ubiquitination 59 K

PTM - dbPAF Gene Name UniProt ID Position Type Species OTUB1; OTB1; OTU1; HSPC263 Q96FW1 16 S Homo sapiens(Human) OTUB1; OTB1; OTU1; HSPC263 Q96FW1 161 S Homo sapiens(Human) OTUB1; OTB1; OTU1; HSPC263 Q96FW1 174 Y Homo sapiens(Human) OTUB1; OTB1; OTU1; HSPC263 Q96FW1 18 S Homo sapiens(Human) OTUB1; OTB1; OTU1; HSPC263 Q96FW1 26 Y Homo sapiens(Human) OTUB1; OTB1; OTU1; HSPC263 Q96FW1 68 Y Homo sapiens(Human)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q96FW1 K188-ac Acetylation GYLQRESkFFEHFIE Q96FW1 S16-p Phosphorylation QKQEPLGsDsEGVNC Q96FW1 S18-p Phosphorylation QEPLGsDsEGVNCLA Q96FW1 Y26-p Phosphorylation EGVNCLAyDEAIMAQ Q96FW1 S55-p Phosphorylation VSERLELsVLYkEYA Q96FW1 Y68-p Phosphorylation YAEDDNIyQQkIkDL Q96FW1 S108-p Phosphorylation LEALLDDskELQRFK Q96FW1 S161-p Phosphorylation SVADLLAsFNDQSTS Q96FW1 Y174-p Phosphorylation TSDYLVVyLRLLTSG Q96FW1 K59-ub Ubiquitination LELsVLYkEYAEDDN Q96FW1 K71-ub Ubiquitination DDNIyQQkIkDLHKK Q96FW1 K73-ub Ubiquitination NIyQQkIkDLHKKYS Q96FW1 K84-ub Ubiquitination KKYSYIRkTRPDGNC Q96FW1 K109-ub Ubiquitination EALLDDskELQRFKA Q96FW1 K188-ub Ubiquitination GYLQRESkFFEHFIE

PTM - dbPTM UniProt ID Position Code Type Resources References Q96FW1 2 A Acetylation Swiss-Prot 1010711 19413330 Q96FW1 16 S Phosphorylation Swiss-Prot 1010711 18669648 Q96FW1 188 K Acetylation Swiss-Prot 1010711 19608861 Q96FW1 16 S Phosphorylation PhosphoELM.10011 18669648 Q96FW1 109 K Ubiquitylation Phosphositeplus.1010730 20639865;21890473;21906983 Q96FW1 188 K Ubiquitylation Phosphositeplus.1010730 21890473 Q96FW1 59 K Ubiquitylation Phosphositeplus.1010730 21906983 Q96FW1 71 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q96FW1 84 K Ubiquitylation Phosphositeplus.1010730 21890473;21906983 Q96FW1 188 K Acetylation Phosphositeplus.1010730 19608861 Q96FW1 16 S Phosphorylation Phosphositeplus.1010730 18669648;20553488;21659604 Q96FW1 18 S Phosphorylation Phosphositeplus.1010730 18491316;20553488 Q96FW1 26 Y Phosphorylation Phosphositeplus.1010730 20553488 Q96FW1 188 K Acetylation HPRD 9.0 19608861 Q96FW1 16 S Phosphorylation HPRD 9.0 18669648 Q96FW1 18 S Phosphorylation HPRD 9.0 18491316

PTM - HPRD Symbol Position Code Type References OTUB1 16 S Phosphorylation 18669648 OTUB1 18 S Phosphorylation 18491316 OTUB1 188 K Acetylation 19608861

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q96FW1 16 S 18669648 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q96FW1 2 N-acetylalanine 19413330;22814378 Q96FW1 16 Phosphoserine 18669648

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type OTUB1 IPI00000581 K 189 YLQRESaKFFEHFI acetylation

PTM - BioGRID Entrez Gene BioGRID ID Position Code Type PMIDs 55611 120751 - K Ubiquitination 16196087 55611 120751 - K Ubiquitination 20639865 55611 120751 - K Ubiquitination 22118674 55611 120751 - K Ubiquitination 18781797 55611 120751 - K Ubiquitination 21987572 55611 120751 - K Ubiquitination 21139048 55611 120751 - K Ubiquitination 21890473 55611 120751 - K Ubiquitination 21906983 55611 120751 - K Ubiquitination 22053931 55611 120751 - K Ubiquitination 22505724 55611 120751 - K Ubiquitination 21963094 55611 120751 - K Ubiquitination 23000965 55611 120751 - K Ubiquitination 24403071 55611 120751 - K Ubiquitination 24654937 55611 120751 - K Neddylation 24654937 55611 120751 - K Sumoylation 24654937 55611 120751 - K Sumoylation 25027693 55611 120751 - K Ubiquitination 25015289 55611 120751 - K Ubiquitination 25147182 55611 120751 - K Ubiquitination 19850743

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID OTUB1 Homo sapiens (Human) 108; 146; 121; 225 "108,DNIYQQKIKDLHK; 146,ALLDDSKELQRFK; 121,KYSYIRKTRPDGN; 225,YLQRESKFFEHFI" 108:21890473; 146:21890473; 121:21890473; 225:21890473 OTUB1 Homo sapiens (Human) 232 "232,YLQRESKFFEHFI" 232:21890473 OTUB1 Homo sapiens (Human) 108; 146; 121; 225 "108,DNIYQQKIKDLHK; 146,ALLDDSKELQRFK; 121,KYSYIRKTRPDGN; 225,YLQRESKFFEHFI" 108:21890473; 146:21890473; 121:21890473; 225:21890473 OTUB1 Homo sapiens (Human) 84; 71; 188; 109 "84,KYSYIRKTRPDGN; 71,DNIYQQKIKDLHK; 188,YLQRESKFFEHFI; 109,ALLDDSKELQRFK" 84:21890473; 71:21890473; 188:21890473; 109:21890473 OTUB1 Homo sapiens (Human) 232 "232,YLQRESKFFEHFI" 232:21890473 OTUB1 Homo sapiens (Human) 118; 80; 93; 197 "118,ALLDDSKELQRFK; 80,DNIYQQKIKDLHK; 93,KYSYIRKTRPDGN; 197,YLQRESKFFEHFI" 84:21906983; 71:21906983; 59:21906983; 109:21906983 OTUB1 Homo sapiens (Human) 232 "232,YLQRESKFFEHFI" 232:21890473