PTM - CPLM Gene Name UniProt ID Type Position Code Prpf8; Prp8 Q99PV0 Acetylation 1463 K Prpf8; Prp8 Q99PV0 Ubiquitination 43 K Prpf8; Prp8 Q99PV0 Ubiquitination 218 K Prpf8; Prp8 Q99PV0 Ubiquitination 267 K Prpf8; Prp8 Q99PV0 Ubiquitination 278 K Prpf8; Prp8 Q99PV0 Ubiquitination 366 K Prpf8; Prp8 Q99PV0 Ubiquitination 428 K Prpf8; Prp8 Q99PV0 Ubiquitination 702 K Prpf8; Prp8 Q99PV0 Ubiquitination 746 K Prpf8; Prp8 Q99PV0 Ubiquitination 1020 K Prpf8; Prp8 Q99PV0 Ubiquitination 1158 K Prpf8; Prp8 Q99PV0 Ubiquitination 1210 K Prpf8; Prp8 Q99PV0 Ubiquitination 1300 K Prpf8; Prp8 Q99PV0 Ubiquitination 1344 K Prpf8; Prp8 Q99PV0 Ubiquitination 1392 K Prpf8; Prp8 Q99PV0 Ubiquitination 1425 K Prpf8; Prp8 Q99PV0 Ubiquitination 1434 K Prpf8; Prp8 Q99PV0 Ubiquitination 1443 K Prpf8; Prp8 Q99PV0 Ubiquitination 1735 K Prpf8; Prp8 Q99PV0 Ubiquitination 1831 K Prpf8; Prp8 Q99PV0 Ubiquitination 1840 K Prpf8; Prp8 Q99PV0 Ubiquitination 1993 K Prpf8; Prp8 Q99PV0 Ubiquitination 2031 K Prpf8; Prp8 Q99PV0 Ubiquitination 2034 K

PTM - dbPAF Gene Name UniProt ID Position Type Species Prpf8; Prp8 Q99PV0 1272 T Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 1273 Y Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 1318 T Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 1404 T Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 1851 S Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 2037 S Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 2042 T Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 2044 T Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 2060 S Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 2079 S Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 2094 S Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 679 S Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 708 T Mus musculus(Mouse) Prpf8; Prp8 Q99PV0 819 S Mus musculus(Mouse)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q99PV0 T708-p Phosphorylation IkQNKARtILQHLSE Q99PV0 S819-p Phosphorylation TTVHWLEsRRFSPIP Q99PV0 T1272-p Phosphorylation TALIGLMtyFREAVV Q99PV0 Y1273-p Phosphorylation ALIGLMtyFREAVVN Q99PV0 T1318-p Phosphorylation FPPVVFYtPKELGGL Q99PV0 T1404-p Phosphorylation IAQNRRLtLEDLEDS Q99PV0 S1851-p Phosphorylation EVAALIRsLPVEEQP Q99PV0 T2044-p Phosphorylation SQLTATQtRTVNKHG Q99PV0 Y2062-p Phosphorylation ITSTTSNyETQTFSS Q99PV0 S2079-p Phosphorylation EWRVRAIsAANLHLR Q99PV0 Y2091-p Phosphorylation HLRTNHIyVSsDDIK Q99PV0 S2094-p Phosphorylation TNHIyVSsDDIKETG Q99PV0 K43-ub Ubiquitination KWQQLQAkRYAEKRK Q99PV0 K218-ub Ubiquitination QPLRDSRkYVNGSTY Q99PV0 K267-ub Ubiquitination LKAFFTSkALNMAIP Q99PV0 K278-ub Ubiquitination MAIPGGPkFEPLVRD Q99PV0 K366-ub Ubiquitination ISHRHSVkSQEPLPD Q99PV0 K428-ub Ubiquitination ALDIPLVkNWYREHC Q99PV0 K702-ub Ubiquitination DMMPEGIkQNKARtI Q99PV0 K746-ub Ubiquitination ILRYVKAkADWWTNT Q99PV0 K785-ub Ubiquitination RLTRLYLkAEQERQH Q99PV0 K1020-ub Ubiquitination NNVVINYkDMNHTNS Q99PV0 K1158-ub Ubiquitination RAVFWDIkNRLPRSV Q99PV0 K1210-ub Ubiquitination SYEEFTHkDGVWNLQ Q99PV0 K1300-ub Ubiquitination NKIQTRIkIGLNSKM Q99PV0 K1344-ub Ubiquitination QSDLRWSkQTDVGIT Q99PV0 K1392-ub Ubiquitination VWAEYALkRQEAIAQ Q99PV0 K1425-ub Ubiquitination RINTLFQkDRHTLAY Q99PV0 K1434-ub Ubiquitination RHTLAYDkGWRVRTD Q99PV0 K1443-ub Ubiquitination WRVRTDFkQYQVLKQ Q99PV0 K1735-ub Ubiquitination QAMAKIMkANPALYV Q99PV0 K1831-ub Ubiquitination TSVWAGQkRLGQLAK Q99PV0 K1840-ub Ubiquitination LGQLAKWkTAEEVAA Q99PV0 K1925-ub Ubiquitination NLYDDWLkTISSYTA Q99PV0 K1993-ub Ubiquitination LILADYGkKNNVNVA Q99PV0 K2031-ub Ubiquitination QQIAEIEkQTkEQSQ Q99PV0 K2034-ub Ubiquitination AEIEkQTkEQSQLTA

PTM - dbPTM UniProt ID Position Code Type Resources References Q99PV0 1318 T Phosphorylation Swiss-Prot 1010711 14729942 Q99PV0 2042 T Phosphorylation Swiss-Prot 1010711 17525332 Q99PV0 2042 T Phosphorylation PhosphoELM.10011 17525332 Q99PV0 2079 S Phosphorylation Phosphositeplus.1010730 18846507 Q99PV0 1318 T Phosphorylation Phosphositeplus.1010730 14729942 Q99PV0 2044 T Phosphorylation Phosphositeplus.1010730 20139300 Q99PV0 2079 S Phosphorylation SysPTM 1.1 18846507

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q99PV0 2042 T 17525332 HTP Phosphorylation

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type Prpf8 IPI00121596 S 2079 WRVRAIpSAANLHL phosphorylation

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID Prpf8 Mus musculus (Mouse) 1158; 1993; 1434; 1831; 1300; 1735; 1425; 1443; 1210; 1392; 1840; 1344; 2031; 2034; 702; 267; 278; 746; 428; 366; 218; 1020; 43 "1158,AVFWDIKNRLPRS; 1993,ILADYGKKNNVNV; 1434,HTLAYDKGWRVRT; 1831,SVWAGQKRLGQLA; 1300,KIQTRIKIGLNSK; 1735,AMAKIMKANPALY; 1425,INTLFQKDRHTLA; 1443,RVRTDFKQYQVLK; 1210,YEEFTHKDGVWNL; 1392,WAEYALKRQEAIA; 1840,GQLAKWKTAEEVA; 1344,SDLRWSKQTDVGI; 2031,QIAEIEKQTKEQS; 2034,EIEKQTKEQSQLT; 702,MMPEGIKQNKART; 267,KAFFTSKALNMAI; 278,AIPGGPKFEPLVR; 746,LRYVKAKADWWTN; 428,LDIPLVKNWYREH; 366,SHRHSVKSQEPLP; 218,PLRDSRKYVNGST; 1020,NVVINYKDMNHTN; 43,WQQLQAKRYAEKR" 1158:22790023; 1993:22790023; 1434:22790023; 1831:22790023; 1300:22790023; 1735:22790023; 1425:22790023; 1443:22790023; 1210:22790023; 1392:22790023; 1840:22790023; 1344:22790023; 2031:22790023; 2034:22790023; 702:22790023; 267:22790023; 278:22790023; 746:22790023; 428:22790023; 366:22790023; 218:22790023; 1020:22790023; 43:22790023