PTM - CPLM Gene Name UniProt ID Type Position Code Upf1; Rent1 Q9EPU0 Acetylation 924 K Upf1; Rent1 Q9EPU0 Ubiquitination 259 K Upf1; Rent1 Q9EPU0 Ubiquitination 277 K Upf1; Rent1 Q9EPU0 Ubiquitination 373 K Upf1; Rent1 Q9EPU0 Ubiquitination 381 K Upf1; Rent1 Q9EPU0 Ubiquitination 434 K Upf1; Rent1 Q9EPU0 Ubiquitination 462 K Upf1; Rent1 Q9EPU0 Ubiquitination 582 K Upf1; Rent1 Q9EPU0 Ubiquitination 633 K Upf1; Rent1 Q9EPU0 Ubiquitination 683 K Upf1; Rent1 Q9EPU0 Ubiquitination 781 K Upf1; Rent1 Q9EPU0 Ubiquitination 785 K Upf1; Rent1 Q9EPU0 Ubiquitination 788 K Upf1; Rent1 Q9EPU0 Ubiquitination 924 K

PTM - dbPAF Gene Name UniProt ID Position Type Species Upf1; Rent1 Q9EPU0 108 Y Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 1084 S Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 1095 S Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 1102 S Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 1105 S Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 1106 T Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 1107 Y Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 1119 T Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 1122 S Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 1124 Y Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 425 S Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 483 Y Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 941 Y Mus musculus(Mouse) Upf1; Rent1 Q9EPU0 953 Y Mus musculus(Mouse)

PTM - PhosphositePlus UniProt ID MOD_RSD Type SITE_+/-7_AA Q9EPU0 K587-ac Acetylation LQkLQQLkDETGELS Q9EPU0 Y108-p Phosphorylation EEDEEDTyYTKDLPV Q9EPU0 T146-p Phosphorylation FCNGRGNtSGSHIVN Q9EPU0 S425-p Phosphorylation DFVWKSTsFDRMQSA Q9EPU0 Y483-p Phosphorylation DLNHSQVyAVKTVLQ Q9EPU0 S560-p Phosphorylation KSREAIDsPVSFLAL Q9EPU0 Y941-p Phosphorylation RFMTTAMyDAREAII Q9EPU0 Y953-p Phosphorylation AIIPGSVyDRSSQGR Q9EPU0 S1084-p Phosphorylation GLSQPELsQDSYLGD Q9EPU0 S1095-p Phosphorylation YLGDEFKsQIDVALs Q9EPU0 S1102-p Phosphorylation sQIDVALsQDstYQG Q9EPU0 S1105-p Phosphorylation DVALsQDstYQGERA Q9EPU0 T1106-p Phosphorylation VALsQDstYQGERAY Q9EPU0 T1119-p Phosphorylation AYQHGGVtGLsQy__ Q9EPU0 S1122-p Phosphorylation HGGVtGLsQy_____ Q9EPU0 Y1124-p Phosphorylation GVtGLsQy_______ Q9EPU0 K195-ub Ubiquitination LLGFIPAkADSVVVL Q9EPU0 K215-ub Ubiquitination CASQSSLkDINWDSS Q9EPU0 K259-ub Ubiquitination ITAQQINkLEELWKE Q9EPU0 K277-ub Ubiquitination ATLEDLEkPGVDEEP Q9EPU0 K373-ub Ubiquitination DEICLRYkGDLAPLW Q9EPU0 K381-ub Ubiquitination GDLAPLWkGIGHVIK Q9EPU0 K434-ub Ubiquitination DRMQSALkTFAVDET Q9EPU0 K462-ub Ubiquitination EVEDVVIkCQLPKRF Q9EPU0 K582-ub Ubiquitination DSMPELQkLQQLkDE Q9EPU0 K599-ub Ubiquitination ELSSADEkRYRALKR Q9EPU0 K633-ub Ubiquitination AGDPRLAkMQFRSIL Q9EPU0 K683-ub Ubiquitination VMCKKAAkAGLSQSL Q9EPU0 K781-ub Ubiquitination TEAANVEkITTkLLk Q9EPU0 K785-ub Ubiquitination NVEkITTkLLkAGAK Q9EPU0 K788-ub Ubiquitination kITTkLLkAGAKPDQ Q9EPU0 K924-ub Ubiquitination MQFSKPRkLVNTVNP

PTM - dbPTM UniProt ID Position Code Type Resources References Q9EPU0 1102 S Phosphorylation Swiss-Prot 1010711 17242355 Q9EPU0 1122 S Phosphorylation Swiss-Prot 1010711 17242355;17525332 Q9EPU0 1102 S Phosphorylation PhosphoELM.10011 17242355 Q9EPU0 1122 S Phosphorylation PhosphoELM.10011 17525332;17242355 Q9EPU0 1084 S Phosphorylation Phosphositeplus.1010730 22503102 Q9EPU0 1102 S Phosphorylation Phosphositeplus.1010730 17242355;21183079;21659604;21659605;22503102 Q9EPU0 1105 S Phosphorylation Phosphositeplus.1010730 17242355;21183079;21659604;21659605 Q9EPU0 1122 S Phosphorylation Phosphositeplus.1010730 18846507;19854140;20139300;21183079;21659604 Q9EPU0 1106 T Phosphorylation Phosphositeplus.1010730 21183079;21659604;21659605 Q9EPU0 1119 T Phosphorylation Phosphositeplus.1010730 21659604 Q9EPU0 108 Y Phosphorylation Phosphositeplus.1010730 18563927 Q9EPU0 1124 Y Phosphorylation Phosphositeplus.1010730 18563927 Q9EPU0 483 Y Phosphorylation Phosphositeplus.1010730 18563927 Q9EPU0 941 Y Phosphorylation Phosphositeplus.1010730 18563927 Q9EPU0 1102 S Phosphorylation SysPTM 1.1 17242355 Q9EPU0 1105 S Phosphorylation SysPTM 1.1 17242355 Q9EPU0 1122 S Phosphorylation SysPTM 1.1 17242355;18846507 Q9EPU0 108 Y Phosphorylation SysPTM 1.1 18563927 Q9EPU0 483 Y Phosphorylation SysPTM 1.1 18563927 Q9EPU0 941 Y Phosphorylation SysPTM 1.1 18563927 Q9EPU0 1124 Y Phosphorylation SysPTM 1.1 18563927

PTM - Phospho.ELM UniProt Position Code PMIDs Kinase Source Type Q9EPU0 1102 S 17242355 HTP Phosphorylation Q9EPU0 1122 S 17525332 HTP Phosphorylation Q9EPU0 1122 S 17242355 HTP Phosphorylation

PTM - UniProt UniProt ID Position Type PMIDs Q9EPU0 1102 Phosphoserine 17242355;21183079 Q9EPU0 1105 Phosphoserine 21183079

PTM - PHOSIDA Symbol Accession Code Position Surrounding Sequence Type Upf1 IPI00420949 S 1102 QIDVALpSQDSTYQ phosphorylation Upf1 IPI00420949 S 1105 VALSQDpSTYQGER phosphorylation Upf1 IPI00420949 S 1122 GGVTGLpSQY____ phosphorylation

PTM - mUbiSiDa Gene Name Species Ubiquitination Site Ubiquitination Sequence Evidence PMID Upf1 Mus musculus (Mouse) 788; 683; 277; 381; 785; 924; 633; 462; 434; 582; 259; 781; 373 "788,ITTKLLKAGAKPD; 683,MCKKAAKAGLSQS; 277,TLEDLEKPGVDEE; 381,DLAPLWKGIGHVI; 785,VEKITTKLLKAGA; 924,QFSKPRKLVNTVN; 633,GDPRLAKMQFRSI; 462,VEDVVIKCQLPKR; 434,RMQSALKTFAVDE; 582,SMPELQKLQQLKD; 259,TAQQINKLEELWK; 781,EAANVEKITTKLL; 373,EICLRYKGDLAPL" 788:22790023; 683:22790023; 277:22790023; 381:22790023; 785:22790023; 924:22790023; 633:22790023; 462:22790023; 434:22790023; 582:22790023; 259:22790023; 781:22790023; 373:22790023